| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 81.1% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 81.1% | 692.9 / 76.1 | 235 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (25/20); new base G (107/83); total (132/103) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 288 total aligned reads displayed.
GTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70672‑70810 | tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttc < 1:6520802/71‑1 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct > 1:2514591/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct > 1:7065115/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt > 1:7287000/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:2474929/70‑1 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:7607786/70‑1 (MQ=255) tACTTTAGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt > 1:15706/1‑71 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt < 1:5842395/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt < 1:515147/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt < 1:177078/70‑1 (MQ=255) cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttc > 1:3739582/1‑71 (MQ=255) ttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc > 1:5322718/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:2297704/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:674157/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc < 1:4694418/70‑1 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:2156462/1‑70 (MQ=255) ccGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGa > 1:4013825/1‑71 (MQ=255) ccGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:2396724/1‑71 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:869776/1‑60 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt > 1:6980122/1‑61 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTTGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg < 1:7626418/71‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg < 1:4860511/71‑1 (MQ=255) cgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAg < 1:3720162/71‑1 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgc > 1:3969817/1‑71 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGaa > 1:7820437/1‑67 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg > 1:6465326/1‑70 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg > 1:5395214/1‑70 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg > 1:2077140/1‑70 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttc < 1:6210493/54‑1 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttc < 1:3621568/54‑1 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:186869/1‑70 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAcc < 1:194752/71‑1 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:2965205/45‑1 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:316455/45‑1 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:1022837/45‑1 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:3241810/1‑59 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:6372933/1‑69 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTAGCCCGGATGAAGCGCAc > 1:7035196/1‑69 (MQ=255) ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAg > 1:75572/1‑71 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt > 1:977292/1‑49 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg > 1:5655040/1‑51 (MQ=255) aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:4592546/45‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCg > 1:3413715/1‑70 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:254755/43‑1 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcg < 1:4745570/60‑1 (MQ=255) gAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGaa > 1:1130696/1‑57 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATGCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:7196333/1‑71 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt > 1:7413132/1‑71 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACTGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:6767225/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:6284173/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:5350979/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg < 1:2900162/57‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc < 1:2248807/70‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa < 1:5792942/71‑1 (MQ=255) cTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt < 1:1819958/38‑1 (MQ=255) ttAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:5074956/1‑71 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGAt < 1:6025701/45‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAg < 1:5441281/49‑1 (MQ=255) aaCTGGCCGTCAATATTTGACAATACGGGTTTCTATCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGa > 1:2420037/1‑71 (MQ=255) aCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAACCGCACCAGCCGCATTTCAGCGAc > 1:898936/1‑71 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc > 1:1380367/1‑52 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGTTGAAgcg > 1:4246186/1‑49 (MQ=39) cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:4945087/1‑51 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAcc > 1:6878239/1‑71 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:4907050/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTC‑‑CGACCTGt < 1:5457921/69‑1 (MQ=255) gTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:3563291/62‑1 (MQ=255) gTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:7479709/70‑1 (MQ=255) gTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACAAGCCGCATTTCAGCGACCTGTTTg < 1:3282664/71‑1 (MQ=255) tCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCg < 1:5762575/51‑1 (MQ=255) tCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGc > 1:3855175/1‑52 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGGAGCGCACCAGc > 1:4222761/1‑47 (MQ=39) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:7881643/1‑47 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:1623575/1‑47 (MQ=255) atatTTGCCAATACGGGTTTCTTTCGCCCGGATGaa > 1:5164140/1‑36 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCg > 1:7772361/1‑47 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:1311783/63‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaaa > 1:3511821/1‑70 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:6954429/1‑71 (MQ=255) tttGCCAATACGGGGTTCTCTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:1159569/71‑1 (MQ=255) ttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc > 1:6622052/1‑38 (MQ=255) gCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa < 1:6934013/70‑1 (MQ=255) gCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt > 1:3344806/1‑71 (MQ=255) ccAATACGGGTTTCTTTCGCCCGGATGAAGCGCAcc < 1:6836271/36‑1 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt > 1:108163/1‑70 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCAACAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt > 1:4438675/1‑70 (MQ=255) aaTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTaa > 1:6071133/1‑71 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc < 1:3042491/71‑1 (MQ=255) aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAcc < 1:7346513/51‑1 (MQ=255) tACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg < 1:5270194/71‑1 (MQ=255) tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg > 1:4066016/1‑47 (MQ=255) tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg < 1:2723455/71‑1 (MQ=255) tACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAATAACg > 1:4478004/1‑71 (MQ=255) aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa > 1:1482690/1‑43 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:2192982/71‑1 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGc < 1:4198132/70‑1 (MQ=255) ggggTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:6322127/52‑1 (MQ=255) ggggTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCg < 1:5545508/71‑1 (MQ=255) gTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCggg > 1:6405814/1‑71 (MQ=255) gTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCggg > 1:1249401/1‑71 (MQ=255) | GTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70672‑70810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |