| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 324,099 | T→A | 5.1% | N164Y (AAC→TAC) ‡ | ykgH ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 324,099 | 0 | T | A | 5.1% | 334.8 / 7.9 | 117 | N164Y (AAC→TAC) ‡ | ykgH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (57/54); new base A (4/2); total (61/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAG > W3110S.gb/324031‑324168 | gTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTcc < 1:502970/71‑1 (MQ=255) tttattttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTgg > 1:2751934/1‑71 (MQ=255) tttattttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTg < 1:5904918/70‑1 (MQ=255) tattttttatttCTGGTGAGCTTGTAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt > 1:4054213/1‑70 (MQ=255) tattttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt > 1:479671/1‑70 (MQ=255) tattttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt < 1:1327689/70‑1 (MQ=255) attttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt > 1:3765111/1‑69 (MQ=255) attttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt > 1:5464355/1‑69 (MQ=255) attttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc < 1:2494340/70‑1 (MQ=255) ttttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCa < 1:7897856/70‑1 (MQ=255) ttttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCa < 1:3398623/70‑1 (MQ=255) ttttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGCTCGGATACAATGACCAGTTCCTGGTCAg > 1:5067299/1‑71 (MQ=255) ttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt < 1:4611900/66‑1 (MQ=255) ttttatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGt < 1:3337353/66‑1 (MQ=255) tatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGtat < 1:1981600/70‑1 (MQ=255) tatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGta < 1:6505608/69‑1 (MQ=255) tatttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGta < 1:5411664/69‑1 (MQ=255) atttCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTatat > 1:362529/1‑71 (MQ=255) tGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGAACAGTTCCTGGTc < 1:66715/58‑1 (MQ=255) gTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAttt < 1:5146346/69‑1 (MQ=255) gTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGGAACTGGTCa < 1:7851740/57‑1 (MQ=38) gAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc > 1:7650417/1‑54 (MQ=255) gAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGGAACTGGTCatt > 1:787176/1‑55 (MQ=37) gAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGGAACTGGTCatt > 1:7137298/1‑55 (MQ=37) aGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGta > 1:3664972/1‑71 (MQ=255) gCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGtat > 1:5818578/1‑58 (MQ=255) gCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGGAACTGGTCatt > 1:355923/1‑53 (MQ=37) cTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGtata < 1:7417687/71‑1 (MQ=255) cTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGtat > 1:4137626/1‑70 (MQ=255) cTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGta < 1:7203212/69‑1 (MQ=255) cTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGta < 1:3357298/69‑1 (MQ=255) ataGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATaaa > 1:5444358/1‑70 (MQ=255) taGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGc > 1:6768291/1‑71 (MQ=255) ttACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc < 1:3292520/44‑1 (MQ=255) ttACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc < 1:738643/44‑1 (MQ=255) ttACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc < 1:2220952/44‑1 (MQ=255) ttACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc < 1:3945463/44‑1 (MQ=255) ttACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTc < 1:4786088/44‑1 (MQ=255) tACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGtata < 1:6235131/50‑1 (MQ=255) tACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCa > 1:3870893/1‑70 (MQ=255) acacAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAg > 1:3782337/1‑44 (MQ=255) acacAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAg > 1:1190554/1‑44 (MQ=255) acacAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAg > 1:4846389/1‑44 (MQ=255) acacAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCAttttt > 1:3501157/1‑57 (MQ=255) acacAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACt > 1:2869678/1‑71 (MQ=255) cacaGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTatat < 1:4181122/49‑1 (MQ=255) acaGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGt > 1:3291442/1‑71 (MQ=255) acaGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGt > 1:7475941/1‑71 (MQ=255) caGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGta < 1:3055245/57‑1 (MQ=255) aGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGta > 1:2037077/1‑43 (MQ=255) aGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCa < 1:4068334/48‑1 (MQ=255) aGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCa < 1:5050805/48‑1 (MQ=255) aGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTa < 1:3266143/71‑1 (MQ=255) gCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTaa > 1:2252998/1‑71 (MQ=255) ccATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAt < 1:3285134/71‑1 (MQ=255) ccATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAt > 1:5656757/1‑71 (MQ=255) ccATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAt > 1:1976815/1‑71 (MQ=255) aTCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATc > 1:1578264/1‑43 (MQ=255) aTCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAtt > 1:6378962/1‑70 (MQ=255) aTCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTg > 1:4122580/1‑71 (MQ=255) aTCACCGTTAGGATACAATGACCAGTTCCTGGTCAGGTATATc > 1:6188938/1‑43 (MQ=255) tCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGc < 1:2383152/71‑1 (MQ=255) cACCGTTCGGATACAATGACCAGTTCCTGGTCAGGtata > 1:6307524/1‑39 (MQ=255) cACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACt > 1:150950/1‑61 (MQ=255) cACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTCTATAAAGCTCACTGTTAATTGCt > 1:6762213/1‑71 (MQ=255) cACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTAGTATAAAGCTCACTGTTAATTGc > 1:6742768/1‑70 (MQ=255) aCCGTTCGGATACAATGACCAGTTCCTGGTCAGGt > 1:577797/1‑35 (MQ=255) aCCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCt > 1:195920/1‑70 (MQ=255) aCCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTg < 1:2842811/71‑1 (MQ=255) aCCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTg < 1:2753962/71‑1 (MQ=255) cGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAtt < 1:3352941/65‑1 (MQ=255) cGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGaa < 1:3885806/71‑1 (MQ=255) ttCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAgg > 1:4856358/1‑71 (MQ=255) tCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCAc > 1:269719/1‑54 (MQ=255) ggATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGaa < 1:4575816/66‑1 (MQ=255) ggATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGaa < 1:2682588/66‑1 (MQ=255) ggATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGt < 1:793138/71‑1 (MQ=255) aTACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATaaa > 1:2674683/1‑44 (MQ=255) aTACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGttt > 1:6573396/1‑71 (MQ=255) tACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCg < 1:1275090/67‑1 (MQ=255) tACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTg > 1:5515914/1‑71 (MQ=255) cAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGt < 1:4467790/70‑1 (MQ=255) aaTGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAt < 1:909908/53‑1 (MQ=255) aaTGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAAt < 1:7327588/53‑1 (MQ=255) aTGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTAtc > 1:169175/1‑71 (MQ=255) gACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTAt < 1:684135/68‑1 (MQ=255) aCCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCa > 1:2879074/1‑71 (MQ=255) ccAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGt < 1:1565525/64‑1 (MQ=255) ccAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCAt < 1:2167766/71‑1 (MQ=255) ccAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCAt > 1:1005418/1‑71 (MQ=255) aGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCa > 1:4145981/1‑38 (MQ=255) aGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCt > 1:1611434/1‑50 (MQ=255) gTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATc > 1:6063285/1‑69 (MQ=255) gTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCga < 1:939846/71‑1 (MQ=255) gTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCga < 1:2166423/71‑1 (MQ=255) ttCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGtt < 1:1612995/41‑1 (MQ=255) ttCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGtt < 1:1705491/41‑1 (MQ=255) ttCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGtt < 1:7042734/41‑1 (MQ=255) ttCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGtt < 1:4576058/41‑1 (MQ=255) tCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCgag > 1:1553213/1‑70 (MQ=255) | GTATTTATTTTTTATTTCTGGTGAGCTTATAGTTACACAGCCATCACCGTTCGGATACAATGACCAGTTCCTGGTCAGGTATATCATTTTTGTATAAAGCTCACTGTTAATTGCTGAAGGCGTTTGTATCTCATCGAG > W3110S.gb/324031‑324168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |