| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 552,689 | T→C | 5.8% | N159D (AAC→GAC) | lpxH ← | UDP‑2,3‑diacylglucosamine pyrophosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 552,689 | 0 | T | C | 5.8% | 191.2 / 2.4 | 69 | N159D (AAC→GAC) | lpxH | UDP‑2,3‑diacylglucosamine pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base T (33/32); new base C (2/2); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAG > W3110S.gb/552620‑552755 | cACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTc > 1:7459948/1‑71 (MQ=255) aCTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcg < 1:7935941/71‑1 (MQ=255) cTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgc < 1:1227738/71‑1 (MQ=255) cTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgc < 1:1784499/71‑1 (MQ=255) gACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgc > 1:6625022/1‑71 (MQ=255) gACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgc > 1:1940296/1‑71 (MQ=255) gACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcg > 1:7135079/1‑70 (MQ=255) gACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTc > 1:7882603/1‑67 (MQ=255) gACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTc > 1:5087390/1‑67 (MQ=255) accaccGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGtt > 1:6805748/1‑65 (MQ=255) accaccGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgcg > 1:162621/1‑71 (MQ=255) accaccGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgcg > 1:7081419/1‑71 (MQ=255) accaccGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgcg > 1:2784148/1‑71 (MQ=255) accaccGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgcg > 1:4318660/1‑71 (MQ=255) cgTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgc < 1:6765880/71‑1 (MQ=255) cgTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgc < 1:5652929/71‑1 (MQ=255) gTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgcg < 1:3012005/71‑1 (MQ=255) ttttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgc < 1:851484/59‑1 (MQ=255) ttttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgc < 1:1137482/59‑1 (MQ=255) tttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgc < 1:2433178/58‑1 (MQ=255) ttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgc > 1:3931654/1‑59 (MQ=255) ttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCgg > 1:7458232/1‑71 (MQ=255) ttGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGAGCGCATTcgcgcg < 1:6592843/70‑1 (MQ=255) gTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGAGGCa < 1:471126/70‑1 (MQ=255) ttAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAAt > 1:3580176/1‑71 (MQ=255) ttAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAAt > 1:5055276/1‑71 (MQ=255) ttAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTCCGCGCGAATGcgcg < 1:7898436/64‑1 (MQ=255) ttAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTCCGCGCGAATGcgcg < 1:7907390/64‑1 (MQ=255) tAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTcgcgc < 1:3924219/54‑1 (MQ=255) aaCGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTCCGCGCGAATGcgcgcg > 1:3645133/1‑64 (MQ=255) aaCGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTCCGCGCGAATGcgcgcg > 1:2605646/1‑64 (MQ=255) cGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcg < 1:2866831/58‑1 (MQ=255) gTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCaa > 1:4252341/1‑65 (MQ=255) gTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGc < 1:1323444/68‑1 (MQ=255) tCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGc < 1:3062142/67‑1 (MQ=255) tCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGttt < 1:393276/71‑1 (MQ=255) tCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGttt < 1:7254085/71‑1 (MQ=255) cATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgc < 1:7340435/71‑1 (MQ=255) aTGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgcg < 1:3994471/71‑1 (MQ=255) tGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGc < 1:2033591/58‑1 (MQ=255) tGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgcg > 1:3908374/1‑70 (MQ=255) gATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCTCGCGCATTCGCGCGGCAATGCGTTTgcg < 1:4608735/69‑1 (MQ=255) aTCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCAtt > 1:3242682/1‑48 (MQ=255) aTCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCAtt > 1:6474855/1‑48 (MQ=255) aTCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcg > 1:4126424/1‑50 (MQ=255) aTCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcg > 1:2902101/1‑50 (MQ=255) aTCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGca > 1:3566630/1‑70 (MQ=255) cAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCAtt < 1:4520274/43‑1 (MQ=255) aGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaacaa > 1:7600056/1‑71 (MQ=255) aGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaacaa > 1:7747204/1‑71 (MQ=255) aGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaacaa > 1:7848802/1‑71 (MQ=255) gCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgc > 1:7721051/1‑44 (MQ=255) cGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCaa > 1:6284690/1‑50 (MQ=255) cGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCaa > 1:5678433/1‑50 (MQ=255) cGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACg > 1:7587482/1‑71 (MQ=255) tttACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACgg > 1:5596694/1‑69 (MQ=255) tACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTgcg > 1:3708174/1‑55 (MQ=255) aCTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAgg < 1:5505991/70‑1 (MQ=255) ctgctgTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAggg < 1:1247436/70‑1 (MQ=255) ctgctgTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAggg < 1:616993/70‑1 (MQ=255) tgctgTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaacaa > 1:4990967/1‑61 (MQ=255) gTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAgg > 1:7221497/1‑70 (MQ=255) ttGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAgg < 1:7378882/69‑1 (MQ=255) ttGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGaa < 1:3831776/71‑1 (MQ=255) ggCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAAt < 1:4502916/70‑1 (MQ=255) tttGCTGTTCGCGCGCATTCGCGCGGCAATGCGttt < 1:4162465/36‑1 (MQ=255) tGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCt < 1:7722934/69‑1 (MQ=255) tGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCt < 1:3718899/69‑1 (MQ=255) gCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGc < 1:1824578/70‑1 (MQ=255) cTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAg > 1:6805371/1‑71 (MQ=255) tCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAg > 1:1450841/1‑67 (MQ=255) | CACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAACAACGGCAGGGCGAGGAATAGCGTCTGCAG > W3110S.gb/552620‑552755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |