| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,079 | C→T | 77.3% | R231R (CGC→CGT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,079 | 0 | C | T | 77.3% | 1075.1 / inf | 546 | R231R (CGC→CGT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (67/57); new base T (201/221); total (268/278) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 548 total aligned reads displayed.
TCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTT > W3110S.gb/71011‑71149 | tCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCAt < 1:6789203/71‑1 (MQ=255)tCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCAt < 1:4466065/71‑1 (MQ=255) gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTAt > 1:4044070/1‑69 (MQ=255) gcgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTAt > 1:4533124/1‑69 (MQ=255) gcgTCTGTCACATCTTTTCCGCCAGCACTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTAtc < 1:6436802/70‑2 (MQ=255) cgTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAg < 1:1848493/71‑1 (MQ=255) cTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGGGGACCAACGTATCAgc > 1:527579/1‑68 (MQ=255) tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAg > 1:3063935/1‑71 (MQ=255) tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAg > 1:2769971/1‑71 (MQ=255) gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg > 1:7441913/1‑71 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg > 1:2908902/1‑70 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGc < 1:3901334/71‑1 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCa < 1:6937968/68‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑g > 1:7058607/1‑70 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑Ga < 1:2248215/71‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGAGCGAGGACCAACGTATCAGCCAGGC‑g > 1:6065402/1‑70 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑g > 1:3252605/1‑69 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAg > 1:1801631/1‑71 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAg > 1:7128489/1‑71 (MQ=255) tCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgc > 1:4891669/1‑71 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgct > 1:6717778/1‑71 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgct > 1:3327433/1‑71 (MQ=255) ttttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCa > 1:7248976/1‑61 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgctgct > 1:6154341/1‑71 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgc < 1:748756/67‑1 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc > 1:4936925/1‑58 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAgctgct > 1:2109283/1‑71 (MQ=255) atCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgctgct > 1:2477702/2‑71 (MQ=255) tCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCtt > 1:966982/1‑71 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg > 1:5979984/1‑58 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg > 1:4128932/1‑58 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCttt < 1:6490979/70‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCttt < 1:6247421/69‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCttt < 1:4394882/69‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTg < 1:770524/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTGAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCtttt > 1:7912094/1‑69 (MQ=255) agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAg > 1:7860614/1‑70 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCtttt < 1:6612720/66‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAg < 1:6882135/69‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCa > 1:1177268/1‑71 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAAAGCATTAGTCAGGC‑GAAg > 1:1973671/1‑57 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCa > 1:508822/1‑68 (MQ=255) gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCAcca < 1:5680903/71‑3 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgctgc < 1:2164711/58‑1 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAgc < 1:7534267/43‑2 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAgctgc > 1:2031039/1‑56 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCAccc > 1:7375673/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTAccc > 1:5174969/1‑71 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑g > 1:5499413/1‑48 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:77411/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:6040258/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTCAGCACCACCCg < 1:5352765/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCg < 1:2379384/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑g < 1:4688555/47‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAg < 1:3158549/61‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAg < 1:6998333/61‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTa > 1:1489684/1‑39 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:5621084/69‑1 (MQ=255) aTTAGCGTCTTAAGCTTGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTg < 1:5774743/57‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt < 1:2827992/71‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt < 1:2576202/71‑1 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCttt < 1:3646883/53‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGcc > 1:6370205/1‑71 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCtttt < 1:2962467/52‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTAt < 1:501700/71‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGa > 1:2672410/1‑62 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCt > 1:4591260/1‑66 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:1685020/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:3780122/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCAACACCCGGATGCCTATCGc > 1:6662380/1‑71 (MQ=255) tAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACt > 1:144095/1‑53 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt > 1:3411411/1‑60 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGcc < 1:2215832/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑Gaa > 1:159841/1‑36 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAg > 1:1005167/1‑37 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc < 1:5922378/70‑1 (MQ=255) aGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa < 1:1561694/70‑1 (MQ=255) cTGGCGCGAGGACCAACGTATCAGCCATGC‑GAAgctgct < 1:4186413/39‑1 (MQ=25) cTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCg < 1:822418/71‑1 (MQ=255) cTGGCGCGAGGACCAACGTATAAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCg > 1:4029946/1‑71 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAcc < 1:1300384/70‑1 (MQ=255) tGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt > 1:1740044/1‑71 (MQ=255) ggCGCGAGGACCAACGTATCAGCCAGGC‑GAAgctgct < 1:5453273/37‑1 (MQ=25) gcgcGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCgg < 1:4667211/52‑1 (MQ=255) gcgcGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGa < 1:777169/53‑1 (MQ=255) cgcgAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcgg < 1:2700694/71‑1 (MQ=255) gcgAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCt < 1:192611/56‑1 (MQ=255) gAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTAt < 1:3513786/56‑1 (MQ=255) gAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtcg > 1:2996399/1‑71 (MQ=255) gACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGcaccac < 1:1110596/41‑1 (MQ=25) gACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCaa < 1:1389460/71‑1 (MQ=255) aCCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:7601427/68‑1 (MQ=255) cAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:4316375/41‑1 (MQ=25) cAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:7549633/71‑1 (MQ=255) cAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:3951936/71‑1 (MQ=255) aaCGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt < 1:4804829/71‑1 (MQ=255) aCGTATCAGCCAGGCGGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt > 1:3738448/1‑71 (MQ=255) aCGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt > 1:2734019/1‑57 (MQ=255) aCGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:931045/1‑71 (MQ=255) cATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt < 1:7633107/71‑1 (MQ=255) | TCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTT > W3110S.gb/71011‑71149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |