| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 609,471 | T→C | 5.5% | intergenic (‑160/+6) | entD ← / ← fepA | phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 609,471 | 0 | T | C | 5.5% | 422.8 / 12.0 | 149 | intergenic (‑160/+6) | entD/fepA | phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex/iron‑enterobactin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base T (68/72); new base C (5/3); total (73/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01 | |||||||||||
Only 100 of 153 total aligned reads displayed.
TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCG > W3110S.gb/609405‑609540 | ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCAt < 1:4467367/71‑1 (MQ=255) ccGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCAt > 1:4119157/1‑69 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTa > 1:7665632/1‑62 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATcgg > 1:4416089/1‑67 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGaa < 1:586060/71‑1 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGt < 1:5034899/71‑1 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTg > 1:2358680/1‑71 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGgt > 1:1223834/1‑71 (MQ=255) gTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGt > 1:6252568/1‑52 (MQ=255) gTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAg < 1:1995854/61‑1 (MQ=255) gTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAg < 1:2178768/61‑1 (MQ=255) gTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGgtgt > 1:7474475/1‑71 (MQ=255) ttCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGtt < 1:6380892/52‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGa < 1:4637372/61‑1 (MQ=255) tCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt > 1:1908663/1‑70 (MQ=255) catgccggATGCGGCGTGAACGCCGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTa > 1:2813699/4‑71 (MQ=37) catgccggATGCGGCGTGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTa > 1:3800433/4‑71 (MQ=37) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTa > 1:2946457/1‑71 (MQ=255) cACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTa > 1:6969354/1‑71 (MQ=255) cgccgcATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACg > 1:1718169/1‑71 (MQ=255) gccgcATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGc < 1:7654689/71‑1 (MQ=255) ccgcATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAg < 1:4237727/54‑1 (MQ=255) cgcATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAg < 1:1689316/47‑1 (MQ=255) cgcATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCt < 1:7447886/70‑1 (MQ=255) cgcATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCt < 1:4021075/70‑1 (MQ=255) cATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGTTGTTTACGCTCat < 1:4239660/71‑1 (MQ=255) cATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCat < 1:369017/71‑1 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCata > 1:6872651/1‑71 (MQ=255) aTCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCat < 1:4559861/70‑1 (MQ=255) tCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCat < 1:1836861/69‑1 (MQ=255) tCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:5316977/68‑1 (MQ=255) tCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCa < 1:5631879/68‑1 (MQ=255) ccGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTa > 1:4833292/1‑61 (MQ=255) cGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTa > 1:3253834/1‑60 (MQ=255) cGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCatat > 1:4351272/1‑69 (MQ=255) cATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGttt < 1:2079068/56‑1 (MQ=255) cATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGttt < 1:5056891/56‑1 (MQ=255) cATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCac < 1:3423054/71‑1 (MQ=255) cATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCac < 1:528128/71‑1 (MQ=255) tGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGa < 1:1456338/43‑1 (MQ=255) tGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGa < 1:7626965/43‑1 (MQ=255) tGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGa < 1:329285/43‑1 (MQ=255) tGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGa < 1:3238108/43‑1 (MQ=255) tGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacg > 1:416687/1‑70 (MQ=255) gAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt < 1:5664479/52‑1 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCa > 1:1603819/1‑39 (MQ=255) aaCGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGtt < 1:3159263/51‑1 (MQ=255) acgacgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTgg > 1:6992482/1‑45 (MQ=255) acgacgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTgg > 1:4927997/1‑45 (MQ=255) acgacgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTgg > 1:3206132/1‑45 (MQ=255) cgacgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgtacgt < 1:2187327/71‑1 (MQ=255) cgacgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgtacgt > 1:110934/1‑71 (MQ=255) gacgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAg > 1:2309041/1‑40 (MQ=255) acgCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTcc < 1:7034687/71‑1 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCg < 1:2465694/71‑1 (MQ=255) gcgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCg < 1:5412467/71‑1 (MQ=255) cgcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:5534423/71‑1 (MQ=255) gcACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGc < 1:3011917/71‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTAc < 1:925556/45‑1 (MQ=255) cACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTAc < 1:6953943/45‑1 (MQ=255) aCTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGgtgt > 1:6388943/1‑40 (MQ=255) cTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCt < 1:680149/46‑1 (MQ=255) cTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCg < 1:1309926/71‑1 (MQ=255) cTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCg > 1:7366537/1‑71 (MQ=255) ttGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTAc > 1:5555082/1‑41 (MQ=255) tGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacg > 1:7589959/1‑54 (MQ=255) tGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTta > 1:6634635/1‑71 (MQ=255) gTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTta < 1:5984502/70‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGc < 1:4844260/40‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTtat < 1:5388897/70‑1 (MQ=255) tCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTtat < 1:4302317/70‑1 (MQ=255) caacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCt > 1:1302467/1‑40 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCg < 1:1379949/59‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCg < 1:3989879/59‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCg < 1:3039766/59‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg < 1:2904530/71‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg < 1:2022847/71‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAg < 1:1472404/70‑1 (MQ=255) aacaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAg < 1:4172284/70‑1 (MQ=255) acaaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCacgta > 1:3543146/1‑51 (MQ=255) caaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGta < 1:730627/71‑1 (MQ=255) aaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCac > 1:2278659/1‑46 (MQ=255) aaTCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCac > 1:5460437/1‑46 (MQ=255) ttCTGATGCTAACGTCAGAAGTGGGTGTTTACGCTCATATACCacgta > 1:5666961/2‑48 (MQ=21) ttCTGATGCTAACGTCAGAAGTGGGTGTTTACGCTCATATACCacgta > 1:6642135/2‑48 (MQ=21) ttCTGATGCTAACGTCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTc > 1:7937092/2‑52 (MQ=25) ttCTGATGCTAACGTCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCg < 1:7248046/59‑1 (MQ=37) tCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAg > 1:2593560/1‑65 (MQ=255) tCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAg > 1:775177/1‑65 (MQ=255) tGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:7089220/53‑1 (MQ=255) tGACGTCAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCgg < 1:7104968/53‑1 (MQ=255) gACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGcc > 1:1596888/1‑71 (MQ=255) aCGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGccc < 1:7561613/71‑1 (MQ=255) aCGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGc < 1:7773303/69‑1 (MQ=255) cGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGcccc > 1:1416213/1‑71 (MQ=255) cGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGcccc > 1:813759/1‑71 (MQ=255) gTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg > 1:6324707/1‑60 (MQ=255) gTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg > 1:4107978/1‑60 (MQ=255) gTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAgg > 1:7690807/1‑60 (MQ=255) gTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCg > 1:6270301/1‑71 (MQ=255) | TAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACGACGCGCACTTTGTCAACAATCTGACGTTAGCATCAGAAGTGGGTGTTTACGCTCATATACCACGTACGTCCCGGCTCGTTATAGGTATACGCCCCG > W3110S.gb/609405‑609540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |