| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,090,238 | A→G | 8.3% | S14S (AGT→AGC) | ycdR ← | predicted enzyme associated with biofilm formation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,090,238 | 0 | A | G | 8.3% | 194.5 / 8.6 | 72 | S14S (AGT→AGC) | ycdR | predicted enzyme associated with biofilm formation |
| Reads supporting (aligned to +/- strand): ref base A (36/30); new base G (4/2); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01 | |||||||||||
AGATTCGCGATCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCA > W3110S.gb/1090169‑1090304 | aGATTCGCGATCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATAc > 1:6487409/1‑71 (MQ=255) tCGCGATCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCAc > 1:7480810/1‑71 (MQ=255) tCGCGATCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCAc > 1:747065/1‑71 (MQ=255) cgcgATCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCAc > 1:657723/1‑70 (MQ=255) aTCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCa > 1:1216895/1‑71 (MQ=255) tCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGCGAGCATAATTATACTCACCAGCAt < 1:7504601/71‑1 (MQ=255) tgGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAg < 1:7820088/69‑1 (MQ=255) tgGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAg > 1:1292783/1‑69 (MQ=255) ggTATAAATGATGTTCTTGACTGGCTATTGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGata < 1:4385127/71‑1 (MQ=255) ggTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGata < 1:3270076/71‑1 (MQ=255) gTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGga > 1:3843308/1‑66 (MQ=255) tataAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCAc > 1:281828/1‑54 (MQ=255) taAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGTATAATTATGCTCAcc > 1:1643600/1‑53 (MQ=37) taAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGTATAATTATGCTCAcc > 1:490244/1‑53 (MQ=37) taAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGTATAATTATGCTCAcc > 1:4160044/1‑53 (MQ=37) taAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCa < 1:6863468/60‑1 (MQ=255) taAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCa < 1:6982343/60‑1 (MQ=255) taAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGAtattt < 1:2571689/70‑1 (MQ=255) atgTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCa < 1:1071019/71‑1 (MQ=255) gTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCAt > 1:4115235/1‑70 (MQ=255) tCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTAc > 1:1742910/1‑71 (MQ=255) cTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGgaga > 1:3490085/1‑54 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATc < 1:1738666/45‑1 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATatttattt > 1:1710273/1‑60 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATatttattt > 1:171668/1‑60 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATatttattt > 1:6640012/1‑60 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGt < 1:5602353/69‑1 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTaa > 1:3195169/1‑71 (MQ=255) gACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTa < 1:6356448/70‑1 (MQ=255) aCTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAAc < 1:3048643/71‑1 (MQ=255) gCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACata < 1:2708432/70‑1 (MQ=255) tAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATAtt > 1:3583862/1‑70 (MQ=255) tAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATAtt > 1:5087123/1‑70 (MQ=255) tAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATAtt > 1:4111926/1‑70 (MQ=255) aTGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTAt > 1:6120156/1‑71 (MQ=255) aTGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTAt > 1:2265542/1‑71 (MQ=255) aTGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTAt < 1:3963970/71‑1 (MQ=255) aTGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTAt < 1:3954628/71‑1 (MQ=255) gCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAAc > 1:6733245/1‑61 (MQ=255) gCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAAc > 1:5964540/1‑61 (MQ=255) aCGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCt > 1:3525962/1‑70 (MQ=255) aCGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCt > 1:4937359/1‑70 (MQ=255) cgcgGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATAttt < 1:3706814/64‑1 (MQ=255) gcgGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTa < 1:7524357/64‑1 (MQ=255) gcgGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTa > 1:6060111/1‑70 (MQ=255) ggTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTaaaa < 1:4302577/71‑1 (MQ=255) gTGAGTATAATTATGCTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTaaaa > 1:3321985/1‑70 (MQ=255) gTGAGTATAATTATGCTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAAt < 1:7792517/71‑1 (MQ=255) gTGAGTATAATTATGCTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAAt < 1:7801709/71‑1 (MQ=255) gTGAGCATAATTATACTCACCAGCATCAGGATATATTTATTTCCATTACGTAACATATTTATCCTTAAAAt > 1:3250917/1‑71 (MQ=255) gTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTcc < 1:5331052/44‑1 (MQ=255) gTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAAt < 1:7138151/71‑1 (MQ=255) gAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCAt > 1:1263635/1‑44 (MQ=255) gAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCt > 1:5842865/1‑71 (MQ=255) aGCATAATTATACTCACCAGCATCAGGAGAtatttat > 1:787330/1‑37 (MQ=255) aGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCt > 1:5805344/1‑70 (MQ=255) gCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAAc > 1:3920633/1‑50 (MQ=255) gCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAAc > 1:2729702/1‑50 (MQ=255) gCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGa < 1:6063648/71‑1 (MQ=255) gCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGa < 1:4204643/71‑1 (MQ=255) gCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGa < 1:3985412/71‑1 (MQ=255) gCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGa < 1:1443013/71‑1 (MQ=255) cATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGaa > 1:5771828/1‑71 (MQ=255) tAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATg > 1:963090/1‑71 (MQ=255) tAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATg > 1:5663366/1‑71 (MQ=255) tAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATg > 1:3227918/1‑71 (MQ=255) ttATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTaaa < 1:7074805/59‑1 (MQ=255) ttATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTaaa < 1:1830520/59‑1 (MQ=255) ttATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCa < 1:6057679/71‑1 (MQ=255) ttATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCa < 1:5384351/71‑1 (MQ=255) ttATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCa < 1:454058/71‑1 (MQ=255) ttATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCa < 1:802671/71‑1 (MQ=255) tataCTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCa > 1:6419873/1‑70 (MQ=255) tataCTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCa > 1:1159295/1‑70 (MQ=255) taCTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGa < 1:3713974/62‑1 (MQ=255) | AGATTCGCGATCCTGTGGCGGTATAAATGATGTTCTTGACTGGCTAATGCACGCGGTGAGCATAATTATACTCACCAGCATCAGGAGATATTTATTTCCATTACGTAACATATTTATCCTTAAAATCTGAATGTCA > W3110S.gb/1090169‑1090304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |