Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,093,6860TC98.3% 194.5 / ‑4.9 58V130A (GTA→GCA) ycdTpredicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (22/35);  minor base A (0/1);  total (22/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTG  >  W3110S.gb/1093620‑1093752
                                                                  |                                                                  
tCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg                                                                <  1:2279002/71‑1 (MQ=255)
        gACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg                                                                <  1:637812/63‑1 (MQ=255)
         aaaTTTCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa                                                        <  1:4710457/68‑1 (MQ=255)
         aCATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa                                                        <  1:3250611/70‑1 (MQ=255)
          cATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg                                                      <  1:3027538/71‑1 (MQ=255)
            ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                    <  1:6998556/71‑1 (MQ=255)
              gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg                                                  >  1:1482259/1‑71 (MQ=255)
                 aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa                                               <  1:6740082/71‑1 (MQ=255)
                 aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa                                               <  1:682528/71‑1 (MQ=255)
                  tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa                                               >  1:6166955/1‑70 (MQ=255)
                      ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                    >  1:7222829/1‑61 (MQ=255)
                      ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa                                              <  1:6319778/67‑1 (MQ=255)
                      ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca                                          >  1:2108538/1‑71 (MQ=255)
                      ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca                                          <  1:6172730/71‑1 (MQ=255)
                        attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt                                                     <  1:1588293/58‑1 (MQ=255)
                        attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt                                                     <  1:1579130/58‑1 (MQ=255)
                         tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                       <  1:920104/71‑1 (MQ=255)
                          ttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                       >  1:5917849/1‑70 (MQ=255)
                           tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa                                                         >  1:2052439/1‑51 (MQ=255)
                            gTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa                                     <  1:2482964/70‑1 (MQ=255)
                               tCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                                 >  1:438388/1‑71 (MQ=255)
                                cGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa                                     >  1:353878/1‑66 (MQ=255)
                                cGTCAGATGAGTTTGTGCATATTAATATTTCTGGAATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc                                 <  1:1682270/70‑1 (MQ=255)
                                 gTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt                                                                 <  1:6331043/37‑1 (MQ=255)
                                 gTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt                                                                 <  1:5497664/37‑1 (MQ=255)
                                  tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                    <  1:618783/49‑1 (MQ=255)
                                   cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt                                                    <  1:7774823/48‑1 (MQ=255)
                                   cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt                                                     <  1:5549714/47‑1 (MQ=255)
                                    aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg                                                      >  1:5341013/1‑45 (MQ=255)
                                    aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAat                            >  1:3966253/1‑71 (MQ=255)
                                    aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAACACAAAACAGCGCAatt                           >  1:7013221/1‑71 (MQ=255)
                                      aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa                                            >  1:1184964/1‑53 (MQ=255)
                                      aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa                                            >  1:2266246/1‑53 (MQ=255)
                                      aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                       <  1:3421671/58‑1 (MQ=255)
                                       tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                       <  1:3241128/57‑1 (MQ=255)
                                       tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                       <  1:1712067/57‑1 (MQ=255)
                                       tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta                         >  1:3991352/1‑71 (MQ=255)
                                       tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                          <  1:5034313/70‑1 (MQ=255)
                                         aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt                       <  1:892394/71‑1 (MQ=255)
                                         aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttat                        <  1:5811548/70‑1 (MQ=255)
                                          gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCaa                             >  1:7671216/1‑64 (MQ=255)
                                          gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt                      <  1:3698561/71‑1 (MQ=255)
                                          gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt                      <  1:6444320/71‑1 (MQ=255)
                                             tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa                                       <  1:2551466/51‑1 (MQ=255)
                                              gtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAacac                                         <  1:5238762/48‑1 (MQ=255)
                                               tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaa                                             >  1:7614216/1‑43 (MQ=255)
                                                gCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaa                 >  1:5924880/1‑70 (MQ=255)
                                                 cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta                         <  1:6706340/61‑1 (MQ=255)
                                                  atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa                                            >  1:5217907/1‑41 (MQ=255)
                                                  atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa              <  1:186888/71‑1 (MQ=255)
                                                  atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa              <  1:5977705/71‑1 (MQ=255)
                                                     ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaaa            <  1:7576114/70‑1 (MQ=255)
                                                      tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt           >  1:7449845/1‑70 (MQ=255)
                                                       aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg          <  1:4843600/70‑1 (MQ=255)
                                                             tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt                       >  1:4670310/1‑51 (MQ=255)
                                                             tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt                          >  1:5957380/1‑48 (MQ=255)
                                                              cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgtg  >  1:5740485/1‑71 (MQ=255)
                                                              cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgt   <  1:2923359/70‑1 (MQ=255)
                                                                  |                                                                  
TCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTG  >  W3110S.gb/1093620‑1093752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: