| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,094,198 | A→T | 9.2% | T301S (ACA→TCA) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,094,198 | 0 | A | T | 9.2% | 237.2 / 14.6 | 87 | T301S (ACA→TCA) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (43/36); new base T (6/2); total (49/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAA > W3110S.gb/1094130‑1094265 | cAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGa > 1:7866283/1‑69 (MQ=255) aaaGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAg < 1:4025253/71‑1 (MQ=255) aGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGa > 1:5927794/1‑66 (MQ=255) aGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGtt > 1:3684331/1‑71 (MQ=255) aGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGtt > 1:5369196/1‑71 (MQ=255) gAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGtt > 1:5260626/1‑70 (MQ=255) aaCATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCa < 1:3242929/71‑1 (MQ=255) aTTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatc < 1:1265467/71‑1 (MQ=255) aTTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAat > 1:7800826/1‑70 (MQ=255) caTCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGTCAGTTCAatcagcatc < 1:4189390/71‑1 (MQ=255) caTCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatc < 1:7906710/71‑1 (MQ=255) caTCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatc < 1:2337804/71‑1 (MQ=255) tCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcag > 1:5306470/1‑71 (MQ=255) tCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcag < 1:612254/71‑1 (MQ=255) cgcgATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcagc > 1:3708425/1‑71 (MQ=255) cgcgATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcagc > 1:838388/1‑71 (MQ=255) aTCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGtt > 1:4274133/1‑53 (MQ=255) aTCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGtt > 1:5204653/1‑53 (MQ=255) aTCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaa < 1:990511/71‑1 (MQ=255) aTCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaa < 1:1250018/71‑1 (MQ=255) aTCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaa < 1:2403571/71‑1 (MQ=255) tCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaa < 1:5640047/71‑1 (MQ=255) tCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaa < 1:2588026/71‑1 (MQ=255) ccTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatc < 1:4000253/61‑1 (MQ=255) ccTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaaaa < 1:2791749/71‑1 (MQ=255) ccTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaaa < 1:6235451/70‑1 (MQ=255) ccTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaaa > 1:337556/1‑70 (MQ=255) cTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCa < 1:5554411/51‑1 (MQ=255) tAACCAATATATTTAAAAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcagc > 1:2107656/1‑61 (MQ=255) aaCCAATATATTTAACAGAAATTATTTTTTTAATGAACTGa > 1:1537387/1‑41 (MQ=255) aaCCAATATATTTAACAGAAATTATTTTTTTAATGAACTGa > 1:1836672/1‑41 (MQ=255) ccAATATATTTAACAGAAATTATTTTTTTATTGAACTGTCAGTTCat < 1:3166086/47‑2 (MQ=37) ccAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCt > 1:51759/1‑71 (MQ=255) atatatTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagc > 1:5630021/1‑49 (MQ=255) atatatTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaaaaa > 1:1048937/1‑63 (MQ=255) tatatTTAACAGAAATTATTTTTTTAATGAACTGAc < 1:4980670/36‑1 (MQ=255) tatatTTAACAGAAATTATTTTTTTAATGAACTGAc < 1:7753353/36‑1 (MQ=255) tatatTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaaa < 1:3244611/60‑1 (MQ=255) tatatTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTAt < 1:1983620/70‑1 (MQ=255) atatTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAat > 1:3636422/1‑43 (MQ=255) tatTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTg < 1:2868335/70‑1 (MQ=255) ttAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCaaaa < 1:3708407/55‑1 (MQ=255) tAACAGAAATTATTTTTTTAATGAACTGACAGTTCAatc > 1:4813536/1‑39 (MQ=255) tAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTc < 1:6024227/70‑1 (MQ=255) tAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCa > 1:7075211/1‑71 (MQ=255) tAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCa > 1:7221252/1‑71 (MQ=255) tAACAGAAATTATTTTTTTAATGAACAGACAGTTCAatc > 1:4048177/1‑39 (MQ=255) aCAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcagc > 1:3509921/1‑46 (MQ=255) aCAGAAATTATTTTTTTAATGAACTGACAGTTCAatcagcatcagc > 1:1117763/1‑46 (MQ=255) aCAGAAATTATTTTTTTAATGAACTGACACTTCAatcagcatcagc > 1:336916/1‑46 (MQ=255) cAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTa > 1:1648284/1‑60 (MQ=255) cAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCAt > 1:3427392/1‑69 (MQ=255) cAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCAt > 1:2421295/1‑69 (MQ=255) aGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGAt < 1:4430023/71‑1 (MQ=255) gAAATTATTTTTTTAATGAACTGACAGTTCAATCTGCATCAGCCCAAAAAACGCCTTATTGCGTCAt > 1:1447152/1‑67 (MQ=255) gAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGAtt < 1:799077/71‑1 (MQ=255) gAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGAt > 1:636854/1‑70 (MQ=255) aaaTTATTTTTTTAATGAACTGACAGTTCAatcagcatc < 1:7617896/39‑1 (MQ=255) aaaTTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTa > 1:2825981/1‑71 (MQ=255) aaTTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGAtt > 1:7646978/1‑69 (MQ=255) aaTTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTAt < 1:2878635/71‑1 (MQ=255) attattTTTTTAATGAACTGACAGTTCAATCAGCATCAGccc > 1:7516445/1‑42 (MQ=255) attattTTTTTAATGAACTGACAGTTCAATCAGCATCAGccc > 1:6547890/1‑42 (MQ=255) attattTTTTTAATGAACTGACAGTTCAATCAGCATCAGccc > 1:2014942/1‑42 (MQ=255) attattTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTAt > 1:5501405/1‑56 (MQ=255) tttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTAtt > 1:46895/1‑53 (MQ=255) tttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt < 1:4560220/57‑1 (MQ=255) tttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt < 1:7146458/57‑1 (MQ=255) ttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTa > 1:3050583/1‑64 (MQ=255) ttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTa > 1:6057511/1‑64 (MQ=255) ttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTa > 1:3138186/1‑64 (MQ=255) ttttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGata > 1:5799972/1‑70 (MQ=255) ttttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATc < 1:5744661/70‑1 (MQ=255) tttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATc < 1:5917833/69‑1 (MQ=255) tttAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGa > 1:2778738/1‑71 (MQ=255) tAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAc < 1:5936622/70‑1 (MQ=255) tAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAc < 1:1999700/70‑1 (MQ=255) tAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGAc > 1:7287901/1‑70 (MQ=255) ttGAACTGTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt > 1:6116108/2‑49 (MQ=255) ttGAACTGTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt > 1:1096298/2‑49 (MQ=255) ttGAACTGTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt > 1:3926085/2‑49 (MQ=255) ttGAACTGTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt > 1:6451035/2‑49 (MQ=255) ttGAACTGTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt > 1:4267788/2‑49 (MQ=255) ttGAACTGTCAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGt > 1:5075095/2‑49 (MQ=255) tGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCAt > 1:1081552/1‑51 (MQ=255) aaCTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTAttc > 1:3169044/1‑42 (MQ=255) aaCTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTc < 1:2538985/71‑1 (MQ=255) aCTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTa > 1:3562882/1‑53 (MQ=255) aCTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACtt < 1:4408803/69‑1 (MQ=255) cTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCaa < 1:4121473/71‑1 (MQ=255) tGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCaa < 1:3186875/70‑1 (MQ=255) | CAAAGAACATTGCACATCGCGATCCCTTAACCAATATATTTAACAGAAATTATTTTTTTAATGAACTGACAGTTCAATCAGCATCAGCCCAAAAAACGCCTTATTGCGTCATGATTATGGATATCGACCACTTCAA > W3110S.gb/1094130‑1094265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |