| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,218,223 | T→A | 7.4% | intergenic (+11/‑102) | ymgB → / → ymgC | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,218,223 | 0 | T | A | 7.4% | 227.0 / 8.9 | 81 | intergenic (+11/‑102) | ymgB/ymgC | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (37/38); new base A (3/3); total (40/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAT > W3110S.gb/1218154‑1218293 | cTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAattatt < 1:4829954/71‑1 (MQ=255)cTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAattatt < 1:1637612/71‑1 (MQ=255) aTGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAg < 1:3309601/71‑1 (MQ=255) gtgATTAGAAAAACACTGGAAATCGTGTTGAGATACACAGCTGATGATATGTAACTTCTAATTATTAAGta > 1:6170359/1‑71 (MQ=255) gATTAGAAAAACACTTGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAattat < 1:5499315/63‑1 (MQ=255) gATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAattat < 1:2605406/63‑1 (MQ=255) gATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAattat < 1:6259390/63‑1 (MQ=255) aTTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAattatt < 1:6936826/63‑1 (MQ=255) aGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGtt > 1:4260427/1‑71 (MQ=255) aGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGtt > 1:6312619/1‑71 (MQ=255) gAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGtt < 1:3456177/70‑1 (MQ=255) gAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGtt < 1:6869511/70‑1 (MQ=255) gAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTATACTTAATAATTAGAAGt < 1:5904878/69‑1 (MQ=25) aaaaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAagtttaagttta < 1:5905985/71‑1 (MQ=255) aaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTta > 1:7762676/1‑69 (MQ=255) aaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTta > 1:4043939/1‑69 (MQ=255) aaaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTta > 1:5623980/1‑69 (MQ=255) aaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAg < 1:72822/71‑1 (MQ=255) aaCACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAg < 1:1436108/71‑1 (MQ=255) acacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGta > 1:7841438/1‑59 (MQ=255) acacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGta > 1:699095/1‑59 (MQ=255) acacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGta > 1:975691/1‑59 (MQ=255) acacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGa < 1:5537798/71‑1 (MQ=255) cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGttt > 1:7819930/1‑65 (MQ=255) cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGaa < 1:227899/71‑1 (MQ=255) cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTATACTTAATAATTAGAAGttac > 1:4536046/1‑64 (MQ=21) cacTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTATACTTAATAATTAGAAGttac > 1:5316384/1‑64 (MQ=21) tGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGaaaa > 1:3762897/1‑70 (MQ=255) tGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAAc > 1:339713/1‑71 (MQ=255) tGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAAc > 1:3684896/1‑71 (MQ=255) gAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGtt < 1:6151840/59‑1 (MQ=255) gAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtc < 1:2550131/71‑1 (MQ=255) aaTCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTta < 1:2153894/59‑1 (MQ=255) aaTCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTta < 1:5647634/59‑1 (MQ=255) aaTCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTta < 1:2918116/59‑1 (MQ=255) aaTCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtcat < 1:4396429/71‑1 (MQ=255) aaTCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtcat < 1:7699811/71‑1 (MQ=255) gtgtTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtcattca > 1:2820014/1‑70 (MQ=255) tgtTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtcattcat > 1:4402613/1‑70 (MQ=255) gtTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGttt < 1:4651752/52‑1 (MQ=255) gtTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGttt < 1:6688921/52‑1 (MQ=255) ttGCGATACACAGCTGATTATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCt < 1:7543406/70‑1 (MQ=255) ttGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGt < 1:2728834/49‑1 (MQ=255) ttGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCt > 1:615799/1‑70 (MQ=255) gCGATACACAGCTGATGATATGTAACTTCTAAttatta > 1:2598859/1‑38 (MQ=255) gATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAttt < 1:5623838/71‑1 (MQ=255) aTACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAtttt < 1:4666071/71‑1 (MQ=255) aTACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAtttt < 1:1369947/71‑1 (MQ=255) tACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtca < 1:4278364/57‑1 (MQ=255) tACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAttttt < 1:2331540/71‑1 (MQ=255) tACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAttttt < 1:6856924/71‑1 (MQ=255) tACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTAttttt < 1:7722735/71‑1 (MQ=255) acacAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGt < 1:3529732/42‑1 (MQ=255) acacAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGt < 1:5071444/42‑1 (MQ=255) acacAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtg > 1:2030201/1‑71 (MQ=255) cacaGCTGATGATATGTAACTTCTAATTATTAAGTATAAg > 1:2177517/1‑40 (MQ=255) cacaGCTGATGATATGTAACTTCTAATTATTAAGTATAAg > 1:1589774/1‑40 (MQ=255) cacaGCTGATGATATGTAACTTCTAATTATTAAGTATAAg > 1:6351234/1‑40 (MQ=255) cacaGCTGATGATATGTAACTTCTAATTATTAAGTATAAg > 1:3927517/1‑40 (MQ=255) cacaGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgt > 1:5354050/1‑71 (MQ=255) acaGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTtata > 1:1951783/1‑45 (MQ=255) acaGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtc < 1:2697953/71‑1 (MQ=255) aGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACtcattc > 1:1633118/1‑55 (MQ=255) gCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACt < 1:2991533/49‑1 (MQ=255) gCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtctg > 1:6986766/1‑70 (MQ=255) atgatATGTAACTTCTAATTATTAAGTATAAGTTta < 1:6489890/36‑1 (MQ=255) atgatATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCt > 1:5702830/1‑71 (MQ=255) atgatATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCt < 1:4561354/71‑1 (MQ=255) tgatATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCt < 1:3016612/70‑1 (MQ=255) tatGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgtctgt > 1:4541322/1‑63 (MQ=255) taaaCTTATACTTAATAATTAGAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGAc > 1:2404236/3‑70 (MQ=25) gTAACTTCTAATTATTAAGTATAAGTTTATAGaaaa > 1:1478641/1‑36 (MQ=255) gTAACTTCTAATTATTAAGTATAAGTTTATAGaaaa > 1:3037038/1‑36 (MQ=255) gTAACTTCTAATTATTAAGTATAAGTTTATAGaaaa > 1:3027862/1‑36 (MQ=255) gTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCtt < 1:4420718/48‑1 (MQ=255) aCTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTa > 1:1259737/1‑46 (MQ=255) aCTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTa > 1:5295045/1‑46 (MQ=255) aCTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTa > 1:5557294/1‑46 (MQ=255) aCTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACtata < 1:5555411/71‑1 (MQ=255) aCTTATACTTAATAATTAGAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACtata < 1:3605096/71‑1 (MQ=37) aCTTATACTTAATAATTAGAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACtata < 1:3614554/71‑1 (MQ=37) cTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCttt > 1:4967469/1‑62 (MQ=255) cTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACtata < 1:6298013/70‑1 (MQ=255) cTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATac < 1:434514/71‑1 (MQ=255) cTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATaca < 1:1429108/69‑1 (MQ=255) cTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATaca > 1:1770393/1‑69 (MQ=255) tAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGc > 1:570792/1‑55 (MQ=255) tAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGc > 1:5041284/1‑55 (MQ=255) attattAACTATAAGTTTATAGAAAACTCATTCATCtt < 1:2362052/38‑1 (MQ=255) tattaAGTATAAGTTTATAGAAAACTCATTCATCTTAtt < 1:7076874/39‑1 (MQ=255) tattaAGTATAAGTTTATAGAAAACTCATTCATCTTAtt < 1:4180937/39‑1 (MQ=255) tattaAGTATAAGTTTATAGAAAACTCATTCATCTTAtt < 1:3629268/39‑1 (MQ=255) tattaAGTATAAGTTTATAGAAAACTCATTCATCTTAtt < 1:3408556/39‑1 (MQ=255) tattaAGTATAAGCTTATAGAAAACTCATTCATCTTAtt < 1:705084/39‑1 (MQ=255) ttaAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTtgt > 1:6396115/1‑42 (MQ=255) ttaAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAt < 1:1220540/71‑1 (MQ=255) | CTGATGTGATTAGAAAAACACTGGAAATCGTGTTGCGATACACAGCTGATGATATGTAACTTCTAATTATTAAGTATAAGTTTATAGAAAACTCATTCATCTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAAT > W3110S.gb/1218154‑1218293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |