| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,225,819 | A→T | 8.8% | intergenic (+335/+37) | ycgI → / ← minE | hypothetical protein/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,225,819 | 0 | A | T | 8.8% | 180.5 / 9.4 | 68 | intergenic (+335/+37) | ycgI/minE | hypothetical protein/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base A (32/30); new base T (4/2); total (36/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
GCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAC > W3110S.gb/1225755‑1225888 | gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc < 1:1445424/71‑1 (MQ=255) aGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc > 1:7360424/1‑69 (MQ=255) aGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt < 1:6113811/71‑1 (MQ=255) gAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt > 1:1258811/1‑70 (MQ=255) aTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg > 1:3670320/1‑69 (MQ=255) tACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg < 1:1044483/71‑1 (MQ=255) cTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAACCTCAAGGCAGAGATAACTCTGCCTTGAAGa < 1:2978467/70‑1 (MQ=255) cTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt < 1:4589808/71‑1 (MQ=255) cTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt < 1:743882/71‑1 (MQ=255) gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa > 1:2149230/1‑71 (MQ=255) ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAct > 1:53215/1‑54 (MQ=255) ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAct > 1:638864/1‑54 (MQ=255) ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt < 1:381470/71‑1 (MQ=255) cgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg > 1:1889594/1‑71 (MQ=255) cgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg > 1:68299/1‑71 (MQ=255) gcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActct > 1:3973679/1‑54 (MQ=255) gcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgc > 1:4348233/1‑71 (MQ=255) gcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgc > 1:7106393/1‑71 (MQ=255) cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcg > 1:3761247/1‑71 (MQ=255) tttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa > 1:2266672/1‑48 (MQ=255) tttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg < 1:125694/61‑1 (MQ=255) tttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcgc > 1:4742371/1‑71 (MQ=255) ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCt > 1:7038584/1‑71 (MQ=255) ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCt > 1:4558819/1‑71 (MQ=255) cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa < 1:5500267/71‑1 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCt > 1:6238972/1‑65 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa > 1:2550394/1‑69 (MQ=255) caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa > 1:6065406/1‑69 (MQ=255) aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg > 1:920132/1‑71 (MQ=255) cGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg > 1:1653794/1‑71 (MQ=255) gTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg < 1:6767975/71‑1 (MQ=255) gTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg < 1:7521284/71‑1 (MQ=255) gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTGTAAAAGCgcat < 1:2121482/70‑4 (MQ=255) aTGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg < 1:5288757/67‑1 (MQ=255) agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAt < 1:827758/70‑1 (MQ=255) gagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAttt < 1:5369527/71‑1 (MQ=255) ggtaTGAAAAACTCAAGGCAGTGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc < 1:3412021/67‑1 (MQ=255) aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGc > 1:3203705/1‑56 (MQ=255) aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAttt < 1:613258/63‑1 (MQ=255) aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGctct < 1:5911357/70‑1 (MQ=255) aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt < 1:7809269/71‑1 (MQ=255) aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc < 1:3069975/71‑1 (MQ=255) aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttct > 1:3259003/1‑70 (MQ=255) aCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg > 1:4951468/1‑51 (MQ=255) aCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg > 1:1444189/1‑70 (MQ=255) aaGGCAGAGATAACTCTGCCTTGAAGATAAATgcg < 1:5975/35‑1 (MQ=255) aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTGTGCTTcc > 1:6997066/1‑71 (MQ=255) aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc > 1:5124286/1‑71 (MQ=255) aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTAc < 1:273651/41‑1 (MQ=21) aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTAc < 1:2004083/41‑1 (MQ=21) aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg > 1:662957/1‑71 (MQ=255) gCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAg > 1:5641261/1‑41 (MQ=25) gCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAg > 1:4130513/1‑41 (MQ=25) gCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg > 1:4177815/1‑70 (MQ=255) gCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg > 1:3557465/1‑70 (MQ=255) gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg < 1:2011373/41‑1 (MQ=255) gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg < 1:2119099/41‑1 (MQ=255) gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg < 1:7240866/41‑1 (MQ=255) gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt < 1:237033/71‑1 (MQ=255) gCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt < 1:809162/71‑1 (MQ=255) agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt > 1:968508/1‑49 (MQ=255) agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt > 1:4836855/1‑49 (MQ=255) agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt > 1:3179636/1‑69 (MQ=255) gagaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg < 1:361630/71‑1 (MQ=255) agaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg > 1:1165348/1‑71 (MQ=255) aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGt < 1:2711735/65‑1 (MQ=255) aTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGt < 1:1279513/70‑1 (MQ=255) tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc < 1:6095792/70‑1 (MQ=255) tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCa > 1:1228936/1‑71 (MQ=255) aaCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc > 1:1831687/1‑48 (MQ=255) aaCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAc > 1:7786626/1‑71 (MQ=255) aaCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAc > 1:897727/1‑71 (MQ=255) | GCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCAC > W3110S.gb/1225755‑1225888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |