| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,250,333 | A→T | 8.3% | V120D (GTT→GAT) | dhaH ← | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,250,333 | 0 | A | T | 8.3% | 266.0 / 14.1 | 96 | V120D (GTT→GAT) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base A (43/45); new base T (5/3); total (48/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGGCGCAG > W3110S.gb/1250263‑1250402 | ttCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa > 1:7766216/1‑71 (MQ=255) tCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:2764037/70‑1 (MQ=255) tCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:1660837/70‑1 (MQ=255) tCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:179899/70‑1 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACtt > 1:2275459/1‑71 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACtt > 1:3358083/1‑71 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:7008566/70‑1 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCAAGCGCATGCATGGCGTCAAAGATAACt < 1:7015524/70‑1 (MQ=255) gtggCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACttt < 1:4290107/71‑1 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg < 1:2952593/71‑1 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg < 1:4635861/71‑1 (MQ=255) gtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg > 1:447149/1‑70 (MQ=255) gtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt > 1:2292102/1‑71 (MQ=255) gtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt > 1:210586/1‑71 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGGGCATGCATGGCGTCAAAGATAACTTTGTc > 1:1084740/1‑71 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATATCTTTGTc > 1:5482042/1‑71 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt < 1:6583428/70‑1 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt < 1:7320531/70‑1 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt < 1:7604461/70‑1 (MQ=255) cGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:1706385/1‑70 (MQ=255) gaggaCGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGat < 1:2749459/71‑1 (MQ=255) ggaCGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGatat < 1:4503497/71‑1 (MQ=255) ggaCGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGatat < 1:4889147/71‑1 (MQ=255) cGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCg > 1:1394334/1‑71 (MQ=255) cGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCg > 1:2608125/1‑71 (MQ=255) gTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:2313013/54‑1 (MQ=255) gTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGcc > 1:1909805/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:3890056/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:5520827/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:3968818/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:1754914/1‑71 (MQ=255) aaaCCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATc > 1:5868368/1‑65 (MQ=255) aCCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa > 1:2868928/1‑50 (MQ=255) cccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGa < 1:1394056/71‑1 (MQ=255) ccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGa < 1:6178527/70‑1 (MQ=255) ccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAg < 1:791887/71‑1 (MQ=255) ccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAg < 1:1263219/71‑1 (MQ=255) aGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAgg > 1:5693256/1‑70 (MQ=255) aGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGc < 1:2220712/71‑1 (MQ=255) aGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGc < 1:4985820/71‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:5590474/44‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:5453100/44‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:3652069/44‑1 (MQ=255) ttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCgcgc < 1:3113135/71‑1 (MQ=255) tCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCt > 1:6840308/1‑71 (MQ=255) tCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCt > 1:3567152/1‑71 (MQ=255) tCACGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCt < 1:95257/42‑1 (MQ=37) tCACGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCt < 1:27713/42‑1 (MQ=37) aCGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGat < 1:4275096/48‑1 (MQ=255) aCGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCttt > 1:1488091/1‑42 (MQ=25) aCGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCttt > 1:2289224/1‑42 (MQ=25) cGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAc > 1:7824322/1‑71 (MQ=255) tttGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg < 1:5930286/40‑1 (MQ=255) tttGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCg < 1:2977470/71‑1 (MQ=255) tGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt > 1:193006/1‑39 (MQ=255) tGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:1973741/1‑40 (MQ=255) cTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccccc < 1:1280106/50‑1 (MQ=255) ttCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGa > 1:33852/1‑38 (MQ=255) ttCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGa > 1:228234/1‑38 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:2180010/1‑35 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:4183617/1‑35 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGa > 1:547179/1‑50 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAc > 1:1608813/1‑62 (MQ=255) ccAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgctg < 1:1436459/69‑1 (MQ=255) aGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAg > 1:4524779/1‑71 (MQ=255) gcatgcatGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCg < 1:1627581/58‑1 (MQ=255) atgcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCg > 1:6161633/1‑41 (MQ=255) gcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTAcc < 1:4830609/70‑1 (MQ=255) gcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCt > 1:1910279/1‑71 (MQ=255) catgGCGTCAAAGATAACTTTGTCGATATCCGccccc < 1:6364699/37‑1 (MQ=255) catgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCtt < 1:1749313/71‑1 (MQ=255) catgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCt < 1:6617587/70‑1 (MQ=255) atgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAg > 1:5931822/1‑62 (MQ=255) atgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTc > 1:1075832/1‑71 (MQ=255) atgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTc < 1:4973968/71‑1 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAgg > 1:2899143/1‑38 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt > 1:6260086/1‑51 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt > 1:1569647/1‑51 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAgtgt > 1:4295170/1‑63 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGa > 1:4990305/1‑71 (MQ=255) gCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGAc > 1:1911695/1‑71 (MQ=255) gTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAgt < 1:1891971/58‑1 (MQ=255) tCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCctcg < 1:5990347/39‑1 (MQ=255) tCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCcgcg < 1:1668208/39‑1 (MQ=255) cAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgct < 1:1943470/50‑1 (MQ=255) cAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgct < 1:529494/50‑1 (MQ=255) aaaGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAAc < 1:6462030/70‑1 (MQ=255) aaGTTATCTTTGACGATATCCGCCCCCGAGGCCGCGCTGACCGTtgc > 1:1966115/1‑47 (MQ=37) aaGATAACTTTGTCGATATCCGCCCCCGAGGCCGCTCTGACCGTTGCTGCCAGTGTACCTTCGACCAACgg < 1:6583904/71‑1 (MQ=255) aaGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgctg > 1:48000/1‑49 (MQ=255) aGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCt > 1:5481585/1‑59 (MQ=255) aGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGa > 1:5328973/1‑63 (MQ=255) gATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGgcg < 1:3652583/71‑1 (MQ=255) gATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGgcg < 1:4254773/71‑1 (MQ=255) aTAACTTTGTCGATATCCGACCCCGAGGCCGCGCTGAACGTTGCTGCCa > 1:4260845/1‑49 (MQ=38) tAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGgcgc < 1:5495604/70‑1 (MQ=255) aaCTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGgcgc < 1:326076/69‑1 (MQ=255) aaCTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGGCgcag > 1:2094459/1‑71 (MQ=255) aaCTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGGCgcag > 1:614815/1‑71 (MQ=255) aaCTTTGTCGATATCCGACCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGGCgcag > 1:6657353/1‑71 (MQ=255) | TTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGACCAACGGCGCAG > W3110S.gb/1250263‑1250402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |