Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6330GT100.0% 308.8 / NA 102Q246Y (CAG→TAToppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/55);  total (47/55)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 102 total aligned reads displayed.

AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAA  >  W3110S.gb/1303567‑1303694
                                                                  |                                                             
aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                           >  1:6195521/1‑71 (MQ=255)
 aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                           >  1:1828394/1‑70 (MQ=255)
 aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                           >  1:7874970/1‑70 (MQ=255)
 aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                          >  1:1030551/1‑71 (MQ=255)
  acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                         >  1:3586643/1‑71 (MQ=255)
    gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                       <  1:148340/71‑1 (MQ=255)
      acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                     <  1:1003866/71‑1 (MQ=255)
       cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                    >  1:874752/1‑71 (MQ=255)
         aaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                                  >  1:6882252/1‑71 (MQ=255)
            cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                               >  1:7746850/1‑71 (MQ=255)
              ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                            <  1:1931629/56‑1 (MQ=255)
              ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                            <  1:4328418/56‑1 (MQ=255)
                  tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTGTTAACTATGTAACCTATTTGCCTATTGCtt                                         >  1:7247196/1‑71 (MQ=255)
                  tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                          >  1:1533148/1‑70 (MQ=255)
                   gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                         <  1:3490298/70‑1 (MQ=255)
                   gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                         <  1:526696/70‑1 (MQ=255)
                   gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                         <  1:7640471/70‑1 (MQ=255)
                   gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                        >  1:4941008/1‑71 (MQ=255)
                    aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                        <  1:5499380/70‑1 (MQ=255)
                    aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                       <  1:3583894/71‑1 (MQ=255)
                     aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:5238199/71‑1 (MQ=255)
                     aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:771089/71‑1 (MQ=255)
                      cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                               >  1:6584512/1‑61 (MQ=255)
                          ggccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                             <  1:3702337/57‑1 (MQ=255)
                          gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                 >  1:61526/1‑55 (MQ=255)
                          gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                 >  1:173976/1‑55 (MQ=255)
                          gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                 <  1:6492319/71‑1 (MQ=255)
                          gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                 <  1:5769593/71‑1 (MQ=255)
                           cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                             <  1:4084973/58‑1 (MQ=255)
                           cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                             <  1:1597132/58‑1 (MQ=255)
                           cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                         <  1:2386806/62‑1 (MQ=255)
                           cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt                                         <  1:4113429/62‑1 (MQ=255)
                            ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               >  1:2525858/1‑71 (MQ=255)
                            ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               >  1:5411760/1‑71 (MQ=255)
                               aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                 <  1:4796544/50‑1 (MQ=255)
                               aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                 <  1:2932503/50‑1 (MQ=255)
                               aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                 <  1:6822451/50‑1 (MQ=255)
                               aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                            <  1:5128951/71‑1 (MQ=255)
                               aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                            >  1:3288812/1‑71 (MQ=255)
                                 cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt                                                        >  1:1082757/1‑41 (MQ=38)
                                 cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt                                                        >  1:3776478/1‑41 (MQ=38)
                                 cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt                                                        >  1:4381486/1‑41 (MQ=38)
                                 cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg                          <  1:594982/71‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                               >  1:3873843/1‑48 (MQ=39)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                               >  1:6343223/1‑48 (MQ=39)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                               >  1:2375103/1‑48 (MQ=39)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:1203823/57‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:5474390/57‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:7874505/57‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:4114672/57‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                      <  1:1774066/57‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                     <  1:552383/58‑1 (MQ=255)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                     <  1:3249279/58‑1 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                        >  1:3739427/1‑54 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                        >  1:3683007/1‑54 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                        >  1:225019/1‑70 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                       <  1:4761056/71‑1 (MQ=255)
                                     tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                    >  1:4504777/1‑57 (MQ=255)
                                       gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc                    >  1:7125889/1‑71 (MQ=255)
                                             cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc              >  1:3845894/1‑71 (MQ=255)
                                              gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg             >  1:7609038/1‑71 (MQ=255)
                                              gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg             >  1:349233/1‑71 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               <  1:2230290/52‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               <  1:5181184/52‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               <  1:1059628/52‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               <  1:6331531/52‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                               <  1:5972263/52‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg             >  1:818244/1‑70 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt            <  1:3461634/71‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt            <  1:6153818/71‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt            <  1:1695926/71‑1 (MQ=255)
                                                gAAAACCGTTGTTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc              >  1:7144315/1‑68 (MQ=255)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                  <  1:330742/48‑1 (MQ=39)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                  <  1:2072141/48‑1 (MQ=39)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                  <  1:6839086/48‑1 (MQ=39)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg             >  1:2818101/1‑69 (MQ=255)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt            <  1:7616828/70‑1 (MQ=255)
                                                gAAAACCGTTATTAACTATGTAACCTATTTCCCTATTGCTTCTGAAGt                                  <  1:515108/48‑1 (MQ=38)
                                                gAAAACCGTTATTAACTATGTAACCTATATGCCTATTGCTTCTGAAGTTAca                              >  1:2034742/1‑51 (MQ=255)
                                                 aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                <  1:2803884/49‑1 (MQ=39)
                                                 aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg          <  1:6272083/71‑1 (MQ=255)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                >  1:1263226/1‑48 (MQ=39)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                >  1:5120707/1‑48 (MQ=39)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                >  1:63058/1‑48 (MQ=39)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                >  1:3380928/1‑48 (MQ=39)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                                >  1:4579021/1‑48 (MQ=39)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg          <  1:1970888/70‑1 (MQ=255)
                                                   aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtg        >  1:5594691/1‑71 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                           >  1:536663/1‑51 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtg        <  1:7159683/70‑1 (MQ=255)
                                                        ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTTGTGaa   <  1:4764311/71‑1 (MQ=255)
                                                        ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa   <  1:7357493/71‑1 (MQ=255)
                                                        ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa   <  1:3080482/71‑1 (MQ=255)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGaa                                    >  1:3768531/1‑36 (MQ=37)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGaa                                    >  1:1909642/1‑36 (MQ=37)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGa                                     <  1:2317717/35‑1 (MQ=37)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa  <  1:941356/70‑1 (MQ=255)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa  <  1:1738941/70‑1 (MQ=255)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa  <  1:6606726/70‑1 (MQ=255)
                                                            taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACAGATGTCAACCGCTACCGTAg          >  1:4613232/1‑60 (MQ=255)
                                                                  |                                                             
AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAA  >  W3110S.gb/1303567‑1303694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: