| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,362,527 | C→T | 7.5% | E32E (GAG→GAA) | puuA ← | gamma‑Glu‑putrescine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,362,527 | 0 | C | T | 7.5% | 250.4 / 7.7 | 80 | E32E (GAG→GAA) | puuA | gamma‑Glu‑putrescine synthase |
| Reads supporting (aligned to +/- strand): ref base C (27/47); new base T (2/4); total (29/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
ACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACA > W3110S.gb/1362458‑1362590 | aCGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCt > 1:5961262/1‑71 (MQ=255) cGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCt < 1:2460558/70‑1 (MQ=255) cTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTc > 1:2069883/1‑69 (MQ=255) ttCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAg > 1:5154041/1‑70 (MQ=255) tCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAgg > 1:145684/1‑70 (MQ=255) tCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAgg > 1:6969154/1‑70 (MQ=255) ccACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAg > 1:6214455/1‑68 (MQ=255) cACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTa < 1:7776656/70‑1 (MQ=255) ggAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAg < 1:559638/64‑1 (MQ=255) ggAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGt > 1:756102/1‑71 (MQ=255) ggAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGt < 1:5716248/71‑1 (MQ=255) gAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGtt < 1:4881047/71‑1 (MQ=255) aGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTc < 1:1316231/71‑1 (MQ=255) gCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCa < 1:2493425/71‑1 (MQ=255) aCCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGt < 1:5474172/64‑1 (MQ=255) aCCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGAGCTCCAGGTAGCGt < 1:6362005/64‑1 (MQ=255) ccATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTc > 1:7493742/1‑71 (MQ=255) ccATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTc < 1:5040642/71‑1 (MQ=255) cATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTcc > 1:1881624/1‑71 (MQ=255) aTTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCa < 1:130633/53‑1 (MQ=255) aTTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTa < 1:7881484/57‑1 (MQ=255) aTTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTa < 1:4850320/57‑1 (MQ=255) aTTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCa > 1:572794/1‑65 (MQ=255) tttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGc < 1:6711040/58‑1 (MQ=255) tttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGc < 1:5884582/58‑1 (MQ=255) tttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGc < 1:5923875/58‑1 (MQ=255) ttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc > 1:2336002/1‑47 (MQ=255) ttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc > 1:7676021/1‑47 (MQ=255) ttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc > 1:16451/1‑47 (MQ=255) ttAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGc > 1:5472261/1‑71 (MQ=255) ttAAATAGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc > 1:1025129/1‑47 (MQ=255) tAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGt > 1:1791818/1‑53 (MQ=255) tAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGt > 1:6333579/1‑53 (MQ=255) tAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGt > 1:3909223/1‑53 (MQ=255) aTCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTa > 1:1046222/1‑70 (MQ=255) ggTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGtt < 1:1921778/53‑1 (MQ=255) ggTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTaa > 1:3135773/1‑68 (MQ=255) ggTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAgg < 1:5765450/71‑1 (MQ=255) ggTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAgg < 1:1177238/71‑1 (MQ=255) gtaagtaaAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGt < 1:3997647/65‑1 (MQ=255) agtaaAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCt < 1:4996916/71‑1 (MQ=255) gtaaAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGgc < 1:3909905/67‑1 (MQ=255) gtaaAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGgc < 1:7550585/67‑1 (MQ=255) gtaaAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGgc < 1:1218253/67‑1 (MQ=255) aaaaCATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGgcgc > 1:144172/1‑67 (MQ=255) aaaCATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAccc > 1:3480958/1‑71 (MQ=255) aaaCATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAccc > 1:4187626/1‑71 (MQ=255) aaaCATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAccc > 1:5751672/1‑71 (MQ=255) aaaCATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAcc < 1:152117/70‑1 (MQ=255) aaCATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAcccc < 1:243700/71‑1 (MQ=255) cATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGc < 1:5029033/71‑1 (MQ=255) cATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGc < 1:5872085/71‑1 (MQ=255) aTCAACATATTGCGTATTCGGGTAGCGCTGCAGGTAGCGTTTCACTTCCt < 1:3453097/50‑1 (MQ=255) aTCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGttt < 1:2213722/42‑1 (MQ=255) aTCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGttt < 1:5101676/42‑1 (MQ=255) aTCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGttt < 1:3428665/42‑1 (MQ=255) tCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGttt < 1:3639823/41‑1 (MQ=255) tCAACATATTGCGTATTAGGGTAGCGCTCCAGGTAGCGttt < 1:178264/41‑1 (MQ=255) aaCATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctct < 1:2066753/71‑1 (MQ=255) aaCATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctc < 1:6347247/70‑1 (MQ=255) aaCATATTGCGTATTAGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCACGCctc > 1:4458351/1‑70 (MQ=255) aaCATATTGCCTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctc > 1:4698986/1‑70 (MQ=255) aCATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctctc < 1:5224879/71‑1 (MQ=255) aCATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctctc < 1:6601480/71‑1 (MQ=255) cATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCTGCctctct > 1:6783317/1‑71 (MQ=255) cATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctctct > 1:6414671/1‑71 (MQ=255) cATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCctctct < 1:4821118/71‑1 (MQ=255) atatTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGt < 1:7094143/48‑1 (MQ=255) atTGCGTATTCGGGTAGCGTTCCAGGTAGCTTTTCACTTCCTGCGTa < 1:1963612/47‑1 (MQ=255) atTGCGTATTCGGGTAGCGTTCCAGGTAGCGTTTCACTTCCTGCGTa < 1:4463538/47‑1 (MQ=255) atTGCGTATTCGAGTAGCGTTCCAGGTAGCGTTTCACTTCCTGCGTa < 1:779782/47‑1 (MQ=38) ttGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCtcttct < 1:1311626/70‑1 (MQ=255) ttGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCtcttct < 1:3270551/70‑1 (MQ=255) ttGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTg < 1:7658783/71‑1 (MQ=255) ttGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTg < 1:2290840/71‑1 (MQ=255) ttGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTg < 1:2173724/71‑1 (MQ=255) tGCGTATTCGGGTAGCGTTCCAGGTAGCGTTTCACTTCCTGCGTaaa > 1:3799195/1‑47 (MQ=255) tGCGTATTCGGGTAGCGTTCCAGGTAGCGTTTCACTTCCTGCGTaaa > 1:7537096/1‑47 (MQ=255) gCGTATTCGGGTAGCGTTCCAGGTAGCGTTTCACTTCCTGCGTa < 1:6047952/44‑1 (MQ=255) cGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAg > 1:3136107/1‑40 (MQ=255) cGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAg > 1:6656621/1‑40 (MQ=255) gTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACa > 1:4651460/1‑70 (MQ=255) tAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCtcttc < 1:2151620/56‑1 (MQ=255) tAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCtcttc < 1:869127/56‑1 (MQ=255) gcTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACa < 1:359414/65‑1 (MQ=255) cTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGcc < 1:6313390/44‑1 (MQ=255) | ACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACA > W3110S.gb/1362458‑1362590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |