| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,427,892 | A→T | 9.8% | intergenic (+198/‑276) | ydaY → / → ynaA | hypothetical protein/predicted tail protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,427,892 | 0 | A | T | 9.8% | 188.9 / 9.3 | 71 | intergenic (+198/‑276) | ydaY/ynaA | hypothetical protein/predicted tail protein |
| Reads supporting (aligned to +/- strand): ref base A (29/35); new base T (4/3); total (33/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCT > W3110S.gb/1427825‑1427961 | cTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACa < 1:5450799/70‑1 (MQ=255) ttCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACa > 1:1827768/1‑67 (MQ=255) tCGGTAATATCCGGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAgaga > 1:7103914/1‑70 (MQ=255) tCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAgaga > 1:351150/1‑70 (MQ=255) tCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAgaga > 1:2439889/1‑70 (MQ=255) cGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGAt > 1:2751175/1‑70 (MQ=255) ggTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGTCAGAGATaa < 1:568896/71‑1 (MQ=255) ggTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGTCAGAGATaa < 1:4571126/71‑1 (MQ=255) ggTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATaa < 1:5294115/71‑1 (MQ=255) ggTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATaa < 1:2214469/71‑1 (MQ=255) tAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAAt < 1:5175586/71‑1 (MQ=255) tAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAAt < 1:4651089/71‑1 (MQ=255) tAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAAt > 1:1256091/1‑71 (MQ=255) aaTATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAAt > 1:7352648/1‑70 (MQ=255) atatCCCGTGCCCGACGTTATGCTTGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATg > 1:7523764/1‑70 (MQ=255) atatCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATaa < 1:3695499/67‑1 (MQ=255) tCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGaa > 1:320798/1‑69 (MQ=255) tCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAat < 1:2205598/70‑1 (MQ=255) cGTGCCTGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAGTGAATAttt < 1:7312190/71‑1 (MQ=255) tGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTa < 1:607103/70‑1 (MQ=255) cacGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGcc < 1:4339248/69‑1 (MQ=255) ccGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGcc < 1:4864369/70‑1 (MQ=255) cGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGAAAGAGATAAATGAATATTTAGCCAc > 1:7258281/1‑71 (MQ=255) gACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATaaa > 1:2753790/1‑55 (MQ=255) gACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATAtt > 1:1109619/1‑63 (MQ=255) gACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATAtt > 1:3864689/1‑63 (MQ=255) gACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAg > 1:3887715/1‑66 (MQ=255) gACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACt > 1:5575264/1‑71 (MQ=255) aCGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGAc < 1:4122113/45‑1 (MQ=255) aCGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACt > 1:2564239/1‑70 (MQ=255) cGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAg > 1:1376680/1‑64 (MQ=255) aTGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCAtgag > 1:6447463/1‑68 (MQ=255) gCTGGAATGGCTGGGGTGCCTTTGCATTTATCTCTGTCAGAGATa > 1:7160863/1‑45 (MQ=25) gCTGGAATGGCTGGGGTGCCTTTGCATTTATCTCTGTCAGAGATa > 1:4655619/1‑45 (MQ=25) tGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCgg < 1:1233705/68‑1 (MQ=255) aTGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattgat > 1:6941771/1‑71 (MQ=255) tGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattgatt < 1:6336350/71‑1 (MQ=255) tGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattgatt < 1:7613150/71‑1 (MQ=255) gCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTAtttattga < 1:4876748/71‑1 (MQ=255) gggTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAgcg < 1:6373869/70‑1 (MQ=255) ggTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGcgcg < 1:6474881/71‑1 (MQ=255) tgcctttgccttTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattg < 1:7722678/59‑1 (MQ=255) tgcctttgccttTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattg < 1:3578179/59‑1 (MQ=255) cctttgcctttATCTCTGACAGAGATAAATGAATAttt > 1:4361293/1‑38 (MQ=255) cctttgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATg < 1:1705239/70‑1 (MQ=255) ctttgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGaa < 1:48703/71‑1 (MQ=255) ctttgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGaa < 1:6536552/71‑1 (MQ=255) tttgcctttATCTCTGACAGAGATAAATGAATATTTAGc < 1:3993025/39‑1 (MQ=255) ttgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAtt > 1:7854137/1‑71 (MQ=255) tgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGaa < 1:2378749/68‑1 (MQ=255) tgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAttt > 1:724205/1‑71 (MQ=255) gcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGaa < 1:1004649/67‑1 (MQ=255) gcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAttt > 1:6284217/1‑70 (MQ=255) gcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAttt < 1:6028357/70‑1 (MQ=255) gcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAttt < 1:2270465/70‑1 (MQ=255) gcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGAGATGAAttt < 1:1255943/70‑1 (MQ=255) ctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGcgcg < 1:5762720/60‑1 (MQ=255) atttATCTCTGTCAGAGATAAATGAATATTTAGCCACTCCTCCGGTATTGATTGAg > 1:6764980/2‑56 (MQ=37) atttATCTCTGTCAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTg > 1:2937126/2‑69 (MQ=255) tttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGt < 1:3074417/45‑1 (MQ=255) ttATCTCTGTCAGAGATAAATGAATATTTAGCCACTCAt < 1:2383875/39‑1 (MQ=255) ttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTat > 1:7750368/1‑46 (MQ=255) tATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAt > 1:1035618/1‑63 (MQ=255) tATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAt > 1:709347/1‑63 (MQ=255) aTCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattgatt < 1:4165019/49‑1 (MQ=255) aTCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTattgatt < 1:2816280/49‑1 (MQ=255) tctGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAt < 1:3731584/59‑1 (MQ=255) tGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAAttt < 1:811630/59‑1 (MQ=255) tGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATc > 1:7232358/1‑71 (MQ=255) tGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATc > 1:6243174/1‑71 (MQ=255) gACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCt > 1:4574316/1‑71 (MQ=255) aCAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCt < 1:3245365/70‑1 (MQ=255) | CTATTCGGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCT > W3110S.gb/1427825‑1427961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |