| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,570,688 | T→C | 5.2% | M506V (ATG→GTG) | gadC ← | predicted glutamate:gamma‑aminobutyric acid antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,570,688 | 0 | T | C | 5.2% | 448.9 / 11.7 | 154 | M506V (ATG→GTG) | gadC | predicted glutamate:gamma‑aminobutyric acid antiporter |
| Reads supporting (aligned to +/- strand): ref base T (72/74); new base C (4/4); total (76/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
Only 100 of 182 total aligned reads displayed.
AGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTG > W3110S.gb/1570619‑1570758 | aGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatc < 1:6426209/71‑1 (MQ=255) gCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:2794249/1‑70 (MQ=255) cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:6373131/1‑71 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCATAATATAg < 1:3574126/71‑1 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg < 1:6992440/71‑1 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg < 1:1957127/71‑1 (MQ=255) ttATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGtgtg < 1:441305/71‑1 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:4858097/1‑71 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:6006968/1‑71 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtgg > 1:1521376/1‑70 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTAAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:3173480/1‑60 (MQ=255) tCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggtg < 1:7899287/71‑1 (MQ=255) gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACAATATAGTGTGGTGa < 1:3750304/70‑1 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata < 1:4106634/56‑1 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt > 1:1759286/1‑71 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt > 1:6511267/1‑71 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg > 1:120033/1‑60 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt < 1:3631090/70‑1 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg > 1:5955710/1‑71 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg < 1:1138325/71‑1 (MQ=255) cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg > 1:5374059/1‑70 (MQ=255) cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg > 1:3133144/1‑70 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAc > 1:1158810/1‑71 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAc > 1:4198284/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:2960331/1‑71 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg > 1:3955386/1‑53 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:1427633/71‑1 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:2839760/71‑1 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:5055124/71‑1 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:7451403/71‑1 (MQ=255) ttATAACCATTCAGACATGGTTAGTGTTTCTTGTCAATCATCACAATATAGTGTGGTGAAc < 1:2282511/61‑1 (MQ=255) tataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcaca < 1:1332657/44‑1 (MQ=255) tataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcaca < 1:7761754/44‑1 (MQ=255) tataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcaca < 1:2623148/44‑1 (MQ=255) tataAACATTCAGTCATGGTTAGTGTTTCTTGTCATTCACCACACTATATtgtgat < 1:1827551/56‑3 (MQ=21) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGaa > 1:5277710/1‑58 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc > 1:1969913/1‑63 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc > 1:880633/1‑63 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGt > 1:4668764/1‑71 (MQ=255) taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAat > 1:5390925/1‑44 (MQ=255) taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGt < 1:4619123/70‑1 (MQ=255) taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTg > 1:5973587/1‑71 (MQ=255) taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTg < 1:2610005/71‑1 (MQ=255) taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTg > 1:2821828/1‑71 (MQ=255) aaCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcaca < 1:6213394/41‑1 (MQ=255) aCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCa < 1:7164524/71‑1 (MQ=255) aCCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATTgtgatg < 1:7009030/53‑1 (MQ=25) ccATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggtg < 1:1275363/52‑1 (MQ=255) ccATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggtg < 1:6733972/52‑1 (MQ=255) ccATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggtg < 1:6176505/52‑1 (MQ=255) ccATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAg < 1:7088958/71‑1 (MQ=255) cATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGc < 1:6970778/68‑1 (MQ=255) cATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAgg < 1:533661/71‑1 (MQ=255) cATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATTGTGATGaa > 1:5484349/1‑53 (MQ=21) cATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATTGTGATGaa > 1:781496/1‑53 (MQ=21) aTTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGaa > 1:3470371/1‑52 (MQ=255) aTTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGga < 1:1333171/71‑1 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:96736/1‑37 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:496740/1‑37 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:4370912/1‑37 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:2850155/70‑1 (MQ=255) aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaagaa < 1:7239681/71‑1 (MQ=255) aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga < 1:1798552/70‑1 (MQ=255) gACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:910984/56‑1 (MQ=255) aCATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGt > 1:1476662/1‑71 (MQ=255) aaTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTg < 1:3123677/70‑1 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa > 1:1698285/1‑71 (MQ=255) tGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc < 1:6812773/71‑1 (MQ=255) ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCggg < 1:5853548/54‑1 (MQ=255) ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCggg < 1:6714094/54‑1 (MQ=255) ttAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAg < 1:2085825/57‑1 (MQ=255) ttAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtt < 1:5835060/71‑1 (MQ=255) tAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt < 1:3254919/42‑1 (MQ=255) aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTg > 1:1116303/1‑42 (MQ=255) aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:3610534/1‑71 (MQ=255) aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:2699583/1‑71 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt < 1:3741286/70‑1 (MQ=255) tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:2023011/57‑1 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:510854/1‑40 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaagaa > 1:1673104/1‑57 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGga > 1:1624208/1‑53 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGga > 1:557403/1‑53 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt < 1:4818370/70‑1 (MQ=255) tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaa > 1:836583/1‑52 (MQ=255) tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGc < 1:4516909/70‑1 (MQ=255) cTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga < 1:6242703/53‑1 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt < 1:7681210/70‑1 (MQ=255) cattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTg > 1:2971329/1‑40 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGc < 1:6081048/39‑1 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGc < 1:3037814/39‑1 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGc < 1:4215227/39‑1 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga > 1:3088641/1‑46 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga > 1:5673745/1‑46 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga > 1:2093564/1‑46 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt < 1:2888764/57‑1 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt < 1:4644591/60‑1 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAg < 1:7667085/40‑1 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg > 1:3514359/1‑71 (MQ=255) catcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTATTGGTGCGTTCTg > 1:343220/1‑66 (MQ=255) tcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTg < 1:7878392/71‑1 (MQ=255) | AGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTG > W3110S.gb/1570619‑1570758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |