| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,583,580 | C→T | 6.3% | R220H (CGT→CAT) ‡ | ydeN ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,583,580 | 0 | C | T | 6.3% | 302.0 / 5.7 | 95 | R220H (CGT→CAT) ‡ | ydeN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (43/46); new base T (3/3); total (46/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCGTTTGT > W3110S.gb/1583514‑1583645 | aaCAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGtt < 1:1411161/71‑1 (MQ=255) tGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGtttt > 1:5677916/1‑68 (MQ=255) tGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAg > 1:3097887/1‑71 (MQ=255) gaGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGt > 1:1616914/2‑71 (MQ=255) aaGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGtt > 1:378759/1‑64 (MQ=255) aaGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGtt > 1:4001354/1‑64 (MQ=255) aaGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAgg < 1:3427935/70‑1 (MQ=255) aaGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAgg < 1:225872/70‑1 (MQ=255) aaGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGt > 1:4022164/1‑71 (MQ=255) ttGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACg < 1:4521134/53‑1 (MQ=255) ttGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttc > 1:5515126/1‑71 (MQ=255) gTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGtt > 1:4290927/1‑62 (MQ=255) gTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCa < 1:3536923/66‑1 (MQ=255) gTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttctt > 1:1573705/1‑71 (MQ=255) tAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttcttc < 1:5689765/71‑1 (MQ=255) tAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttctt < 1:142639/70‑1 (MQ=255) tAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttctt < 1:3316718/70‑1 (MQ=255) aaTATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTg < 1:1916568/55‑1 (MQ=255) aaTATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTg < 1:5149254/55‑1 (MQ=255) aaTATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTg < 1:1562263/55‑1 (MQ=255) aaTATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttcttc < 1:4808822/70‑1 (MQ=255) tatGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGtt < 1:4185527/50‑1 (MQ=255) tatGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCAttc < 1:1987182/65‑1 (MQ=255) tGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCg > 1:4461089/1‑68 (MQ=255) cGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaa < 1:128196/71‑1 (MQ=255) ccTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGa > 1:2293436/1‑45 (MQ=255) ccTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAtgt > 1:1949718/1‑71 (MQ=255) ccTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAtg > 1:1964725/1‑70 (MQ=255) ccTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAtg < 1:5622255/70‑1 (MQ=255) ccTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAt > 1:3542388/1‑69 (MQ=255) cTGCAGCGTGGAATCTCATAAAGTAATCAAAGCCACGGt < 1:5839476/39‑1 (MQ=255) cAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCg > 1:1576407/1‑57 (MQ=255) cAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCg > 1:4700640/1‑57 (MQ=255) cAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCg > 1:2609237/1‑57 (MQ=255) gTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaa < 1:7209337/58‑1 (MQ=255) tGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAg < 1:270569/37‑1 (MQ=255) tGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAg < 1:7674799/37‑1 (MQ=255) tGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCa < 1:3831895/44‑1 (MQ=255) tGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCa < 1:4984526/44‑1 (MQ=255) tGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCa < 1:6045849/44‑1 (MQ=255) tGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGtt < 1:7576591/71‑1 (MQ=255) ggAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaaa > 1:5664345/1‑58 (MQ=255) aaTCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaa < 1:6001086/54‑1 (MQ=255) aaTCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaa < 1:6430803/54‑1 (MQ=255) aaTCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaa < 1:3077900/54‑1 (MQ=255) aaTCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGt > 1:4842763/1‑70 (MQ=255) aTCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCa > 1:7938394/1‑71 (MQ=255) tCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCAt > 1:4670889/1‑71 (MQ=255) ccATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGa > 1:2721647/1‑71 (MQ=255) cATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCg < 1:617384/44‑1 (MQ=255) cATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGAt < 1:474212/71‑1 (MQ=255) aTAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGa > 1:2359569/1‑69 (MQ=255) tAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaaa < 1:6061026/49‑1 (MQ=255) tAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAg > 1:2127334/1‑71 (MQ=255) aaaGTAATCAAAGCCACGGTTTTGAGGTTGCCAttctt > 1:2126481/1‑38 (MQ=255) aaaGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGt > 1:6853791/1‑71 (MQ=255) aaGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaaa > 1:3896786/1‑47 (MQ=255) aaGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaaa > 1:1649981/1‑47 (MQ=255) aaGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTGCCGCAGAAAATGTGGTGAAGTTGTCATGATAGt > 1:7198253/1‑70 (MQ=255) aGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCa > 1:3979236/1‑71 (MQ=255) taataaAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGa < 1:5529710/63‑1 (MQ=255) tAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaaa < 1:260593/44‑1 (MQ=255) tAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaaa < 1:3734190/44‑1 (MQ=255) aaTCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCAcgc > 1:5371538/1‑71 (MQ=255) aTCAAAGCCACGGTTTTGAGGTTGGCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCAcgcg > 1:4726796/1‑71 (MQ=255) aTCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCAcgcg > 1:5044664/1‑71 (MQ=255) aTCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCAcgcg > 1:2584644/1‑71 (MQ=255) aTCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCAcgcg > 1:7598995/1‑71 (MQ=255) tCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaaa < 1:6294090/40‑1 (MQ=255) tCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAt > 1:38420/1‑42 (MQ=255) tCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAt > 1:4291067/1‑42 (MQ=255) tCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgt < 1:5370382/71‑1 (MQ=255) tCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgt < 1:6924354/71‑1 (MQ=255) tCAAAACCGTGGCTTTGAGGTTGCCATTCTTCCGCAGaaaa < 1:6894251/41‑1 (MQ=21) tCAAAACCGTGGCTTTGAGGTTGCCATTCTTCCGCAGaaaa < 1:7124721/41‑1 (MQ=21) tCAAAACCGTGGCTTTGAGGTTGCCATTCTTCCGCAGaaaa < 1:3195172/41‑1 (MQ=21) tCAAAACCGTGGCTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGTTGAAGTTGTCATGAt > 1:4855475/1‑61 (MQ=37) tCAAAACCGTGGCTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGAt > 1:1492066/1‑61 (MQ=38) tCAAAACCGTGGCTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGAt > 1:4393201/1‑61 (MQ=38) cAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaa < 1:5160233/38‑1 (MQ=255) cAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGaa < 1:2874409/38‑1 (MQ=255) cAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgtt > 1:1694283/1‑71 (MQ=255) aaaGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgttt > 1:832500/1‑71 (MQ=255) aaaGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgttt > 1:462848/1‑71 (MQ=255) aaaGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgtt < 1:1636290/70‑1 (MQ=255) aaGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgtttg < 1:627765/71‑1 (MQ=255) aaGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgttt < 1:5826531/70‑1 (MQ=255) aGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATg < 1:3372258/55‑1 (MQ=255) aGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTc > 1:1042227/1‑61 (MQ=255) aGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTc > 1:6570556/1‑61 (MQ=255) aGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgtttgt < 1:1877072/71‑1 (MQ=255) aGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCgtttgt < 1:3268177/71‑1 (MQ=255) gCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCAt < 1:3033778/53‑1 (MQ=255) gCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCAcg < 1:7092227/63‑1 (MQ=255) ccACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAAt > 1:3422606/1‑36 (MQ=255) | AACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGGTTTTGAGGTTGCCATTCTTCCGCAGAAAATGTGGTGAAGTTGTCATGATAGTCACGCGTTTGT > W3110S.gb/1583514‑1583645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |