| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,659,609 | A→T | 5.3% | intergenic (+25/‑174) | ynfD → / → ynfE | hypothetical protein/oxidoreductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,659,609 | 0 | A | T | 5.3% | 215.8 / 3.4 | 76 | intergenic (+25/‑174) | ynfD/ynfE | hypothetical protein/oxidoreductase subunit |
| Reads supporting (aligned to +/- strand): ref base A (33/39); new base T (2/2); total (35/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATG > W3110S.gb/1659541‑1659677 | ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGTatat < 1:5490349/70‑1 (MQ=255)ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat > 1:2013496/1‑70 (MQ=255)ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat > 1:2113141/1‑70 (MQ=255)ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat < 1:2287893/70‑1 (MQ=255)ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGata < 1:3612878/69‑1 (MQ=255) cgcAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGAc > 1:1571254/1‑70 (MQ=255) gcAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACa > 1:4592458/1‑70 (MQ=255) gcAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACa > 1:260662/1‑70 (MQ=255) aGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAAt < 1:3397337/71‑1 (MQ=255) aGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAAt < 1:3116916/71‑1 (MQ=255) tCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATcc > 1:635609/1‑71 (MQ=255) tCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATcc > 1:4124584/1‑71 (MQ=255) cTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCg > 1:6769075/1‑71 (MQ=255) cTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCg > 1:2762958/1‑71 (MQ=255) tAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCgg > 1:5752596/1‑71 (MQ=255) tAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCgg > 1:6438097/1‑71 (MQ=255) tAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCgg > 1:6447306/1‑71 (MQ=255) tAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCgg > 1:3602005/1‑71 (MQ=255) tAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCgg > 1:2946915/1‑71 (MQ=255) gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCg < 1:2303064/68‑1 (MQ=38) gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATc < 1:297128/66‑1 (MQ=255) gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATc < 1:5692329/66‑1 (MQ=255) gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCg < 1:3496815/68‑1 (MQ=255) ccAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACa < 1:7320326/61‑1 (MQ=255) cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCg > 1:5551894/1‑66 (MQ=255) aGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAtt > 1:1312980/1‑71 (MQ=255) aGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAtt > 1:7750757/1‑71 (MQ=255) ggATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATc > 1:6250750/1‑62 (MQ=255) ggATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAttt > 1:6264807/1‑71 (MQ=255) gcgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat < 1:663712/49‑1 (MQ=255) gcgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc < 1:5361663/70‑1 (MQ=255) gcgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACa > 1:6994427/1‑71 (MQ=255) cgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACa > 1:2898138/1‑70 (MQ=255) gcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTg < 1:2857769/65‑1 (MQ=255) cgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATac < 1:367781/71‑1 (MQ=255) cgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATac < 1:6228825/71‑1 (MQ=255) gcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATac > 1:3141447/1‑70 (MQ=255) tGCCGATCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATacaac < 1:4611776/70‑1 (MQ=255) tGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATg < 1:7764482/43‑1 (MQ=255) tGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATg < 1:2730468/43‑1 (MQ=255) tGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATcc < 1:3853946/51‑1 (MQ=255) tGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATcc < 1:2242572/51‑1 (MQ=255) tGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATacaaca > 1:2764904/1‑71 (MQ=255) gCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAt > 1:5674882/1‑56 (MQ=255) ccGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATacaaca < 1:4965419/69‑1 (MQ=255) ccGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATacaaca < 1:2885520/69‑1 (MQ=255) gAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCg > 1:7017804/1‑48 (MQ=25) cAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGa > 1:2981791/1‑51 (MQ=255) gTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCtt < 1:5381865/71‑1 (MQ=255) gTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCtt < 1:7088610/71‑1 (MQ=255) gTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCt > 1:2740863/1‑70 (MQ=255) tAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAttt < 1:748844/46‑1 (MQ=255) acacATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt < 1:4439356/71‑1 (MQ=255) acacATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt < 1:3862338/71‑1 (MQ=255) acacATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttat < 1:4177389/70‑1 (MQ=255) acacATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttat < 1:7410301/70‑1 (MQ=255) acacATTATCGACTGAACGCCGGATAAGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttat > 1:3523984/1‑70 (MQ=255) caTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACAt < 1:4263754/54‑1 (MQ=255) caTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt < 1:7610873/68‑1 (MQ=255) ttATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATc > 1:4969510/1‑56 (MQ=255) ttATCGACTGAACGCCGGATATGAAAAATCCGGCGATTTGAACATACAACATAATCCCACCttatta > 1:2404060/1‑67 (MQ=255) aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt < 1:7021548/70‑1 (MQ=255) tCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATa < 1:5709111/70‑1 (MQ=255) tCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATAc > 1:3895719/1‑71 (MQ=255) cTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCtt > 1:4468933/1‑71 (MQ=255) aaCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATaa > 1:5493212/1‑44 (MQ=255) aaCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTc < 1:5800203/69‑1 (MQ=255) aaCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTc < 1:4140405/69‑1 (MQ=255) aCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTa < 1:4793747/70‑1 (MQ=255) aCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAt < 1:2855376/71‑1 (MQ=255) cGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTATTCATACCCTTCTAtt > 1:7247639/1‑71 (MQ=255) cGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAtt < 1:4912921/71‑1 (MQ=255) ccGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACt < 1:5161990/55‑1 (MQ=37) ccGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGa < 1:3412591/71‑1 (MQ=255) cGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt < 1:5670731/51‑1 (MQ=255) cGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt < 1:600856/51‑1 (MQ=255) cGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGat < 1:5232603/71‑1 (MQ=255) cGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGat < 1:1922881/71‑1 (MQ=255) ggATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGata > 1:7396876/1‑71 (MQ=255) ggATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGata > 1:6745855/1‑71 (MQ=255) ggATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTAatc < 1:4418867/71‑2 (MQ=255) gATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt > 1:6160229/1‑55 (MQ=255) atatGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATg > 1:4827057/1‑71 (MQ=255) tatGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTa > 1:4234647/1‑62 (MQ=255) tatGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATg > 1:1667073/1‑70 (MQ=255) | CCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATG > W3110S.gb/1659541‑1659677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |