| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,683,336 | T→A | 9.2% | L216Q (CTG→CAG) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,683,336 | 0 | T | A | 9.2% | 176.9 / 8.1 | 65 | L216Q (CTG→CAG) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base T (24/35); new base A (3/3); total (27/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCT > W3110S.gb/1683266‑1683405 | gAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCt > 1:1071571/1‑71 (MQ=255)gAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCt > 1:210307/1‑71 (MQ=255) tCGACAACAGCCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCg < 1:2241962/71‑1 (MQ=255) cGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa > 1:342961/1‑71 (MQ=255) gACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGa < 1:2568627/70‑1 (MQ=255) acaGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTAATCGACCAGGTCGAtcact < 1:1770696/71‑1 (MQ=38) aGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttactta < 1:1806978/71‑1 (MQ=255) aGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttactta > 1:5597229/1‑71 (MQ=255) cgcTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttact > 1:5227999/1‑70 (MQ=255) cTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttacttac < 1:5126771/71‑1 (MQ=255) aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTAcc > 1:4047369/1‑69 (MQ=255) aaTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAg > 1:4793214/1‑71 (MQ=255) aTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTAcc < 1:6808258/68‑1 (MQ=255) aTAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGt > 1:734542/1‑71 (MQ=255) tAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAt < 1:130760/55‑1 (MQ=255) tCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttact < 1:7017093/59‑1 (MQ=255) tCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttact < 1:4836137/59‑1 (MQ=255) tCACTGATGAAAACCGCGCCTGGAGAGAGAGAAGTGATCGACCTGGTCGAttacttact < 1:2691018/59‑1 (MQ=255) tgatgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttact < 1:5671488/55‑1 (MQ=255) atgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTg > 1:2846874/1‑62 (MQ=255) atgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCAc > 1:3012278/1‑71 (MQ=255) tgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac < 1:7778372/51‑1 (MQ=255) tgaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac < 1:7915812/51‑1 (MQ=255) gaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttactta < 1:3991789/53‑1 (MQ=255) gaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCg > 1:5828251/1‑71 (MQ=255) gaAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCg > 1:4518804/1‑71 (MQ=255) aaaaCCGCGCCTGGCGAGAAAGAAGTGATCGACCt > 1:2450936/1‑35 (MQ=255) aaaaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg > 1:5910059/1‑37 (MQ=255) aaaaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttact < 1:4872397/46‑1 (MQ=255) aaaaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCg < 1:375413/70‑1 (MQ=255) aaaCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCt > 1:6273206/1‑60 (MQ=255) aCCGCTCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACg < 1:4135084/71‑1 (MQ=255) ccGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTAcc < 1:5681772/50‑1 (MQ=255) cgcgCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCa < 1:5829692/71‑1 (MQ=255) gcgcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAg > 1:4163008/1‑71 (MQ=255) cgcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGa > 1:1023030/1‑65 (MQ=255) gcCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttactta < 1:4415420/44‑1 (MQ=255) gcCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttactta < 1:6629190/44‑1 (MQ=255) gcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGtt < 1:7699561/71‑1 (MQ=255) gcCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGtt < 1:5771522/71‑1 (MQ=255) tGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAg < 1:2828774/66‑1 (MQ=255) gCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCa < 1:7916406/71‑1 (MQ=255) gCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCa < 1:3492613/71‑1 (MQ=255) cGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTACCa > 1:1145420/1‑70 (MQ=255) cGAGAAAGAAGTGATCGACATGGTTGATTACTTACTTACCAGTTGCTTTGTCAcc < 1:700524/55‑1 (MQ=255) gagaAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGc < 1:7132107/45‑1 (MQ=255) gaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTc > 1:5083430/1‑49 (MQ=255) gaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACtt > 1:7175647/1‑70 (MQ=255) gaaagaaGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACtt > 1:717490/1‑70 (MQ=255) agaagTGATCGACCTGGTCGATTACTTACTTACCAGt > 1:2702889/1‑37 (MQ=255) agTAATCGACCAGGTCGATCACTTACTTACCAGTTGCt < 1:7218221/38‑1 (MQ=14) tGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgt < 1:2725350/71‑1 (MQ=255) gATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAg > 1:5792018/1‑51 (MQ=255) gATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGTCGgtc > 1:6229160/1‑71 (MQ=255) aaTCGACCAGGTCGATCACTTACTGACCAGTTGCTTTGTCACCGGACGCa > 1:7475028/2‑50 (MQ=25) aTCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATgg < 1:7720455/65‑1 (MQ=255) aTCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATgg < 1:1145172/65‑1 (MQ=255) aTCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATgg < 1:4105053/65‑1 (MQ=255) tCGACCAGGTCGATCACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACt > 1:5647434/1‑58 (MQ=39) cGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgt > 1:4686814/1‑70 (MQ=255) cGACCAGGTCGATCACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgt > 1:3209235/1‑70 (MQ=255) gACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgtc < 1:7021083/70‑1 (MQ=255) gACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGgtcgtc < 1:734081/70‑1 (MQ=255) gACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCa > 1:6389292/1‑71 (MQ=255) aCCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCAt < 1:6570227/71‑1 (MQ=255) ccTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATc < 1:5096888/71‑1 (MQ=255) cTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATg > 1:3443898/1‑58 (MQ=255) cTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCt < 1:327235/71‑1 (MQ=255) tGGTCGATTACTTACTTTCCAGTTGCTTTGTCACCGGa > 1:4436466/1‑38 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGc > 1:1923306/1‑41 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACt < 1:197967/52‑1 (MQ=255) tGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCg < 1:7389270/60‑1 (MQ=255) | GAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCT > W3110S.gb/1683266‑1683405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |