Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,746,008 T→A 7.3% F280I (TTT→ATT)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,746,0080TA 7.3% 310.5 / 13.6 110F280I (TTT→ATT) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (51/51);  new base A (6/2);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 124 total aligned reads displayed.

TGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGT  >  W3110S.gb/1745938‑1746076
                                                                      |                                                                    
tGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACt                                                                      >  1:4395132/1‑71 (MQ=255)
tGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACt                                                                      >  1:233837/1‑71 (MQ=255)
 gCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACt                                                                      <  1:6416277/70‑1 (MQ=255)
  ccGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTTAACttt                                                                    <  1:4670655/71‑1 (MQ=255)
  ccGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACttt                                                                    <  1:3377569/71‑1 (MQ=255)
  ccGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACtt                                                                     <  1:6551379/70‑1 (MQ=255)
   cgtcgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACtt                                                                     >  1:6018282/1‑69 (MQ=255)
    gtcgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATAGCCCTGAACta                                                                     >  1:4813091/1‑67 (MQ=255)
      cgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGtt                                                                >  1:7035941/1‑71 (MQ=255)
       gtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGtt                                                                <  1:1255580/70‑1 (MQ=255)
       gtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGtt                                                                <  1:5250608/70‑1 (MQ=255)
          gCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACt                                                            <  1:5171525/71‑1 (MQ=255)
          gCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTAAAGTTCag                                                             <  1:4259525/70‑2 (MQ=255)
              tGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATg                                                        >  1:2020581/1‑71 (MQ=255)
                ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATg                                                        <  1:2590057/69‑1 (MQ=255)
                ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATg                                                        <  1:2088173/69‑1 (MQ=255)
                  aGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCg                                                    >  1:7040404/1‑71 (MQ=255)
                   gtgtCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGt                                                   <  1:7876384/71‑1 (MQ=255)
                    tgtCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTc                                                     <  1:4931693/68‑1 (MQ=255)
                       ctcCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCa                                                              >  1:7660724/1‑56 (MQ=255)
                       ctcCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCtt                                               >  1:1874364/1‑71 (MQ=255)
                       ctcCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTAAAGTTCagg                                                            >  1:477960/1‑56 (MQ=255)
                       ctcCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTAAAGTTCagg                                                            >  1:3153850/1‑56 (MQ=255)
                          cGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGtt                                                                >  1:5649802/1‑51 (MQ=255)
                          cGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAAt                                                         >  1:252493/1‑58 (MQ=255)
                          cGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAAt                                                         >  1:6353498/1‑58 (MQ=255)
                          cGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTc                                              <  1:3046366/69‑1 (MQ=255)
                           gCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCATGAACTTTAGTTCACTAATGTTCGTGCTTCCaa                                           <  1:6962731/71‑1 (MQ=255)
                            cTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAAt                                          <  1:1175890/71‑1 (MQ=255)
                            cTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAAt                                          <  1:7813226/71‑1 (MQ=255)
                             tCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATg                                         >  1:7027320/1‑71 (MQ=255)
                              cGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGt                                        >  1:2829649/1‑71 (MQ=255)
                               ggTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTc                                       <  1:6974278/71‑1 (MQ=255)
                                gTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTc                                       <  1:7342369/70‑1 (MQ=255)
                                 tATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACttt                                                                    <  1:4431283/40‑1 (MQ=255)
                                 tATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACttt                                                                    <  1:4386613/40‑1 (MQ=255)
                                 tATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGt                                                   >  1:4981553/1‑57 (MQ=255)
                                   ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCa                                                              <  1:1459110/44‑1 (MQ=255)
                                   ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCt                                    <  1:4123700/70‑1 (MQ=255)
                                   ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTg                                   >  1:3629285/1‑71 (MQ=255)
                                   ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTg                                   >  1:7503507/1‑71 (MQ=255)
                                   ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTg                                   >  1:6372782/1‑71 (MQ=255)
                                     gttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGtt                                                                <  1:3941109/40‑1 (MQ=255)
                                     gttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGtt                                                                <  1:1193945/40‑1 (MQ=255)
                                     gttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGt                                                       >  1:5613654/1‑49 (MQ=255)
                                     gttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGt                                                       >  1:1698320/1‑49 (MQ=255)
                                     gttgATGTCGCAGGACACCAGATTACCCTGAACTTTAGTTCACTAATGt                                                       >  1:2702811/1‑49 (MQ=255)
                                      ttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAg                                                                  >  1:5117356/1‑37 (MQ=255)
                                      ttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAg                                                                  >  1:6967524/1‑37 (MQ=255)
                                          tGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTggcggc                              <  1:4581825/69‑1 (MQ=255)
                                           gTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGc                           >  1:7692650/1‑71 (MQ=255)
                                             cGCAGGACACCAGATTGCCCTGAACATTAGTGAACTaaag                                                        <  1:2163741/40‑3 (MQ=18)
                                             cGCAGGACACCAGATTGCCCTGAACATTAGTGAACTaaag                                                        <  1:6610041/40‑3 (MQ=18)
                                              gCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGc                           >  1:1486992/1‑68 (MQ=255)
                                              gCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGc                           >  1:4095656/1‑68 (MQ=255)
                                              gCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCgg                         >  1:7863857/1‑70 (MQ=255)
                                              gCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGt                        <  1:3302284/71‑1 (MQ=255)
                                               cAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGtt                                                      >  1:1011271/1‑40 (MQ=17)
                                               cAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGtt                                                      >  1:2306258/1‑40 (MQ=17)
                                               cAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGtt                                                      >  1:5502172/1‑40 (MQ=17)
                                               cAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGt                                                       >  1:5948181/1‑39 (MQ=17)
                                               cAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGt                                                       >  1:3628221/1‑39 (MQ=17)
                                                  gACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                    <  1:3757491/71‑1 (MQ=255)
                                                     acCAGATTGCCCTGAACTTTAGTTGACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATc                 <  1:876131/71‑1 (MQ=255)
                                                     acCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAg                            <  1:6862821/60‑1 (MQ=255)
                                                     acCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATc                 <  1:7713019/71‑1 (MQ=255)
                                                     acCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATc                 <  1:124230/71‑1 (MQ=255)
                                                     acCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATc                 <  1:5613051/71‑1 (MQ=255)
                                                      ccAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCg                          <  1:6258067/61‑1 (MQ=255)
                                                      ccAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATc                 <  1:4087423/70‑1 (MQ=255)
                                                       cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGc                                                 >  1:7571647/1‑37 (MQ=255)
                                                       cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGc                                                 >  1:6126037/1‑37 (MQ=255)
                                                       cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAg                            >  1:7494249/1‑58 (MQ=255)
                                                       cAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCcg               >  1:4321650/1‑71 (MQ=255)
                                                         gATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCcgcg             >  1:4221528/1‑71 (MQ=255)
                                                          aTTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTg                                   <  1:1645725/48‑1 (MQ=255)
                                                          aTTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTg                                   <  1:3383458/48‑1 (MQ=255)
                                                          aTTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGt            <  1:2715100/71‑1 (MQ=255)
                                                             gCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGAGGCAGCGGTAACTATCCGCGTAg          <  1:2845032/70‑1 (MQ=255)
                                                               ccTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTggcggc                              <  1:4183616/48‑1 (MQ=255)
                                                               ccTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTa           <  1:2349045/67‑1 (MQ=255)
                                                                cTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATg                                         >  1:3366092/1‑36 (MQ=255)
                                                                cTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTggcggc                              <  1:4690615/47‑1 (MQ=255)
                                                                cTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGTCGGCAGCGGTAACTATCCGCGTAGGTTa      <  1:3643394/71‑1 (MQ=255)
                                                                cTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGt                        <  1:1813759/53‑1 (MQ=255)
                                                                cTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTa      <  1:5924873/71‑1 (MQ=255)
                                                                cTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTa      <  1:1491624/71‑1 (MQ=255)
                                                                  gAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCa                             >  1:4255535/1‑46 (MQ=255)
                                                                  gAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc    >  1:6142352/1‑71 (MQ=255)
                                                                  gAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc    >  1:5148377/1‑71 (MQ=255)
                                                                  gAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc    >  1:3092577/1‑71 (MQ=255)
                                                                  gAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc    >  1:4756688/1‑71 (MQ=255)
                                                                   aaCTTTAGTTCACTAATGTTCGTGCTTCCAATGTGGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc    <  1:2714952/70‑1 (MQ=255)
                                                                   aaCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTa                       >  1:773948/1‑51 (MQ=255)
                                                                   aaCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc    <  1:591674/70‑1 (MQ=255)
                                                                   aaCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCg   >  1:2331468/1‑71 (MQ=255)
                                                                    aCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCg   >  1:6793359/1‑70 (MQ=255)
                                                                    aCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGt  <  1:7189405/71‑1 (MQ=255)
                                                                    aCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGt  <  1:3147848/71‑1 (MQ=255)
                                                                     cTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                    <  1:4811079/52‑1 (MQ=255)
                                                                      |                                                                    
TGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGT  >  W3110S.gb/1745938‑1746076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: