| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,883,072 | T→A | 7.3% | D151V (GAC→GTC) | yeaT ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,883,072 | 0 | T | A | 7.3% | 265.5 / 11.2 | 96 | D151V (GAC→GTC) | yeaT | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (45/44); new base A (4/3); total (49/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
AATTCCGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAA > W3110S.gb/1883003‑1883141 | aaTTCCGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGt > 1:731699/1‑70 (MQ=255) aTTCCGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCa < 1:3097073/71‑1 (MQ=255) aTTCCGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCa < 1:2451942/71‑1 (MQ=255) ttCCGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCAt > 1:6381524/1‑71 (MQ=255) cGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGc < 1:664519/71‑1 (MQ=255) gCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGcc > 1:7477751/1‑70 (MQ=255) gCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCa < 1:4355382/71‑1 (MQ=255) gACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAAt > 1:5556569/1‑71 (MQ=255) aCGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATg < 1:4371092/71‑1 (MQ=255) gcAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGaa > 1:1782082/1‑71 (MQ=255) gcAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGa > 1:4899829/1‑70 (MQ=255) cAGAGAATAAGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAat < 1:5592141/71‑1 (MQ=255) agagAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAata < 1:7427439/71‑1 (MQ=255) agagAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAata < 1:7431768/71‑1 (MQ=255) agagAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAat > 1:4779303/1‑70 (MQ=255) agaATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATc > 1:5052831/1‑71 (MQ=255) agaATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATc < 1:4315240/71‑1 (MQ=255) gaATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCg < 1:4275492/71‑1 (MQ=255) aTACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgag < 1:7754743/71‑1 (MQ=255) aTACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgag < 1:4301882/71‑1 (MQ=255) aTACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgag < 1:2921035/71‑1 (MQ=255) tACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgaga < 1:6026886/71‑1 (MQ=255) tACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgaga < 1:412985/71‑1 (MQ=255) tACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgaga < 1:4324115/71‑1 (MQ=255) tACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgaga < 1:2774559/71‑1 (MQ=255) aCGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATc > 1:7805785/1‑66 (MQ=255) aCGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATc > 1:5803636/1‑66 (MQ=255) cGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGAt < 1:169450/70‑1 (MQ=255) ggTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCg < 1:4593600/71‑1 (MQ=255) gTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGa > 1:2409008/1‑71 (MQ=255) tAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATg < 1:2814966/54‑1 (MQ=255) aTTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGacac > 1:7024860/1‑71 (MQ=255) tGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCt > 1:1694202/1‑71 (MQ=255) tGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCt > 1:7462452/1‑71 (MQ=255) tGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCt > 1:3287428/1‑71 (MQ=255) ggTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCAt > 1:2194006/1‑41 (MQ=255) ggTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCg > 1:5515439/1‑51 (MQ=255) gTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCgaga < 1:31070/60‑1 (MQ=255) gTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCtt < 1:7274087/70‑1 (MQ=255) gTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCtt < 1:6858204/70‑1 (MQ=255) gTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCtt < 1:7589340/70‑1 (MQ=255) tCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCga > 1:5106202/1‑57 (MQ=255) tCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCa < 1:7025303/71‑1 (MQ=255) ccAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt > 1:745224/1‑70 (MQ=255) cAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt < 1:4392277/69‑1 (MQ=255) ttGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGTTCGACACCTTCATTGACCa > 1:6909059/1‑70 (MQ=255) cGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCaa < 1:3070391/68‑1 (MQ=255) gggCGATTAAATTAGGGGCGATGTCATCGCCAATGCGaa > 1:6389505/1‑39 (MQ=255) cggCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCg < 1:4797197/70‑1 (MQ=255) gCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGa > 1:4961673/1‑61 (MQ=255) gCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGAc > 1:6297422/1‑71 (MQ=255) aTTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCg < 1:6833226/39‑1 (MQ=255) aTTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCg < 1:3352020/39‑1 (MQ=255) ttAAATTATGGGCGATGTCATCGCCAATGCGAATATCGAGAt > 1:7452897/1‑42 (MQ=255) ttAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGAt > 1:7194984/1‑42 (MQ=255) ttAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCaa < 1:5709643/61‑1 (MQ=255) ttAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCaa < 1:6550218/61‑1 (MQ=255) ttAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGt < 1:7325288/71‑1 (MQ=255) tAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTc > 1:5670241/1‑71 (MQ=255) aaaTTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCg > 1:3666112/1‑71 (MQ=255) aaTTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCgg > 1:747462/1‑71 (MQ=255) aTTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCtt < 1:847449/47‑1 (MQ=255) aTTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCtt < 1:608882/47‑1 (MQ=255) tAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGAc > 1:4603689/1‑61 (MQ=255) tAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGAc > 1:2122029/1‑61 (MQ=255) aGGGGCGATGTCATCGACAATGCGAATATCGAGATCGa > 1:6177206/1‑38 (MQ=255) ggggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCTGTCGGTCTTc < 1:6334417/71‑1 (MQ=255) ggggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCtt < 1:2274459/70‑1 (MQ=255) tggCGATGACATCGCCAATGCGAATATCGAGATCGACACCt < 1:1621296/40‑1 (MQ=255) ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTcc < 1:2956100/70‑1 (MQ=255) ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCca > 1:1558841/1‑71 (MQ=255) gCGATGACATCGCCAATGCGAATATCGAGATCGACACCtt < 1:7390603/40‑1 (MQ=255) gCGATGACATCGCCAATGCGAATATCGAGATCGACACCtt < 1:1051007/40‑1 (MQ=255) gATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt < 1:1169897/42‑1 (MQ=255) gATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt < 1:5866725/42‑1 (MQ=255) gATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt < 1:5774066/42‑1 (MQ=255) gATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt < 1:5576024/42‑1 (MQ=255) gATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCAtt < 1:179458/42‑1 (MQ=255) gATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACAt > 1:5602408/1‑71 (MQ=255) gATGACATCGCCAATGCGAATATCGAGATCGACACCTTCa > 1:1623318/1‑40 (MQ=255) gATGACATCGCCAATGCGAATATCGAGATCGACACCTTCa > 1:7841247/1‑40 (MQ=255) gATGACATCGCCAATGCGAATATCGAGATCGACACCTTCa > 1:7295784/1‑40 (MQ=255) gATGACATCGCCAATGCGAATATCGAGATCGACACCTTCa > 1:1846662/1‑40 (MQ=255) tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGa > 1:5406343/1‑42 (MQ=255) tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGAc > 1:2221352/1‑43 (MQ=255) tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACAt > 1:3717470/1‑69 (MQ=255) tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACAt > 1:6230001/1‑69 (MQ=255) tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATc > 1:4018397/1‑70 (MQ=255) tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCa < 1:3406428/71‑1 (MQ=255) gTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGa > 1:503542/1‑41 (MQ=255) gTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGa > 1:5295867/1‑41 (MQ=255) gTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGa > 1:5674781/1‑41 (MQ=255) gTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCa > 1:3705936/1‑70 (MQ=255) gTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCa > 1:7645201/1‑70 (MQ=255) gTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCa > 1:7913679/1‑70 (MQ=255) tCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCa > 1:2308237/1‑52 (MQ=255) tCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCa > 1:7702737/1‑52 (MQ=255) tCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCa > 1:5946217/1‑52 (MQ=255) tCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCaa < 1:2742452/70‑1 (MQ=255) tCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCaa < 1:4260257/70‑1 (MQ=255) | AATTCCGGCGACGCGCAGAGAATACGGTAATTGGTCGCCAGTTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAA > W3110S.gb/1883003‑1883141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |