Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 654214–655411 655412 2–1199 49 [0] [0] 49 [dcuC]–[dcuC] [dcuC],insH,[dcuC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 6542130 (0.000)34 (0.820) 24/92 NT 100% coding (70/477 nt) dcuC anaerobic C4‑dicarboxylate transport
?W3110S.gb 655413 = 0 (0.000)coding (978/1092 nt) dcuC anaerobic C4‑dicarboxylate transport

ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654140‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  W3110S.gb/655413‑655480
                                                                                                                                              
ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         <  1:3161683/71‑1
   AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                      >  1:3406194/1‑71
    GGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCGGACTTTGAATAAAGCCGATGGTGCTAAGC                                                                     <  1:2061006/71‑1
         ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                 >  1:3115982/1‑70
            aTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                             >  1:74890/2‑71
            CTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                             <  1:143743/71‑1
            CTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         >  1:1751683/1‑59
             TCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                             >  1:4235286/1‑70
             TCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCC                                                                    <  1:2100271/63‑1
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                          >  1:2470984/1‑71
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            <  1:2961373/69‑1
               GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAG                                                              <  1:1789796/67‑1
                  GAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATAC                                                       <  1:560006/71‑1
                    ACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTG                                                                <  1:781601/60‑1
                     CCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAAT                                                         >  1:1999608/1‑66
                     CCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                          >  1:1663288/1‑65
                      CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCATGA                                                               <  1:1908814/59‑1
                       AAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGC                                                                     <  1:3274769/52‑1
                         ACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCG                                                >  1:1102943/1‑71
                         ACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCC                                                                  >  1:2872543/1‑53
                          CGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCAGCGGC                                              <  1:2848376/71‑1
                             GGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTG                                                                <  1:3367369/51‑1
                             GGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGC                                                                     <  1:3320523/46‑1
                                 GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCA                                         <  1:1053386/70‑1
                                  CGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGC                                       <  1:3666102/71‑1
                                  CGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGC                                       <  1:305988/71‑1
                                  CGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAG                                                              <  1:635817/48‑1
                                  CGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAG                                                              <  1:794728/48‑1
                                    ATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGA                                                               <  1:3700532/45‑1
                                    ATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGA                                                               <  1:3708027/45‑1
                                     TAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGC                                    <  1:1252140/71‑1
                                      AGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGC                                              <  1:4205304/60‑1
                                        AAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCAT                                  >  1:2795524/1‑70
                                          ATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGC                                                 >  1:510030/1‑53
                                           TCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCC                                                     >  1:4198862/1‑48
                                               ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          <  1:1473840/71‑1
                                                 TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          >  1:341546/1‑69
                                                 TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          >  1:4069213/1‑69
                                                     AATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGAAGCATCACCACGTTAGCA                     <  1:666901/70‑1
                                                         AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGC                <  1:1048408/71‑1
                                                          AGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGG                                               >  1:4249758/1‑39
                                                               ATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGC          <  1:196661/71‑1
                                                               ATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAA                                            >  1:2422901/1‑37
                                                                TGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCC         <  1:2112705/71‑1
                                                                 GGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA        >  1:1088887/1‑71
                                                                  GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA        <  1:3042385/70‑1
                                                                      TAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                     <  1:4005808/53‑1
                                                                       AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCG   >  1:3050271/1‑70
                                                                       AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCT            >  1:2444067/1‑61
                                                                        AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  1:3989253/1‑70
                                                                         GCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA        <  1:2937203/63‑1
                                                                                                                                              
ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654140‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  W3110S.gb/655413‑655480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.