Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178180 2178181 2–1199 84 [0] [0] 81 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)64 (1.540) 43/92 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTC  >  W3110S.gb/2178182‑2178248
                                                                                                                                              
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                         <  1:4038976/71‑1
  TCGTTATCATCATCCGCTTCCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTT                                                                       <  1:3864582/71‑1
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                     >  1:1555675/1‑71
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                     <  1:471546/71‑1
       ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                  >  1:2291420/1‑71
       ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                  >  1:1779608/1‑71
          ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                <  1:3732381/70‑1
             ATCCGCTTGCTGAAAATGAACTATATTTGTTGGCCATAACAGATAAATGGCTGACGACTTAGCATGAATC                                                             <  1:1571861/70‑1
                CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCC                                                          >  1:2667296/1‑70
                 GCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                         >  1:4141971/1‑70
                 GCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                         >  1:2693660/1‑70
                   TTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCA                                                       >  1:257432/1‑70
                    TGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                              >  1:306326/1‑62
                    TGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                        <  1:3255075/52‑1
                     GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                    >  1:2196589/1‑71
                       TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                    >  1:3056437/1‑69
                       TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                     <  1:4231797/52‑1
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                >  1:3341357/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                >  1:3339262/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                >  1:4156999/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                >  1:3430319/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                <  1:909737/71‑1
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                >  1:587718/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                <  1:779643/71‑1
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                    >  1:1266357/1‑67
                          AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                               >  1:4270374/1‑71
                           AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                >  1:3084915/1‑69
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                            >  1:1932935/1‑71
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                             <  1:2198563/70‑1
                               AACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTG                                          >  1:1843156/1‑71
                                 CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                        <  1:3549166/71‑1
                                 CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGT                                              <  1:3585175/65‑1
                                 CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                <  1:580885/47‑1
                                  TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                       >  1:2155072/1‑71
                                  TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                               <  1:2214480/63‑1
                                  TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                <  1:1605661/46‑1
                                   TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                       >  1:3014024/1‑70
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                       >  1:794934/1‑69
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                       >  1:49804/1‑69
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                        >  1:3576395/1‑36
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                         >  1:4159064/1‑35
                                      TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGC                                   >  1:39843/1‑71
                                      TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                    <  1:319323/70‑1
                                        TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                   >  1:2080229/1‑37
                                         GTTTGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                    <  1:2100200/51‑1
                                           TGGCCTTAACAGATAAATGGCTGATGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                <  1:2823462/69‑1
                                           TGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCT                              >  1:3349984/1‑71
                                               CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                          <  1:3610260/71‑1
                                                  cACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                        <  1:2922438/69‑1
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       >  1:3853435/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       >  1:1166923/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       >  1:1733017/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCA                         >  1:1912333/1‑69
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                    >  1:2013357/1‑42
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       >  1:3157452/1‑70
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       <  1:345234/70‑1
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       <  1:1450963/70‑1
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                  >  1:1269527/1‑59
                                                    CAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCA                         <  1:3934229/67‑1
                                                     AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                    <  1:2938017/71‑1
                                                     AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                    <  1:795462/71‑1
                                                     AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                    <  1:2007429/71‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                    >  1:338791/1‑70
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                  <  1:62794/56‑1
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGC                      <  1:2800203/67‑1
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       <  1:1220192/66‑1
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                    >  1:713950/1‑37
                                                         AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                             >  1:396313/1‑42
                                                         AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                             >  1:1757601/1‑42
                                                          AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                        <  1:1849085/62‑1
                                                          AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                    >  1:3621446/1‑50
                                                           ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC              <  1:854243/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT           <  1:296724/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT           <  1:1974771/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG            >  1:1831718/1‑70
                                                               CTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG            >  1:2123066/1‑69
                                                               CTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                       <  1:2811481/58‑1
                                                                TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                   <  1:1972446/61‑1
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG        >  1:1563233/1‑71
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCA                     >  1:1585344/1‑57
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGC                      >  1:3755418/1‑56
                                                                    GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA     <  1:3957030/71‑1
                                                                    GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA     <  1:1889190/71‑1
                                                                    GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA     <  1:36935/71‑1
                                                                    GACTTAGCATGAATCCCCTCACAATGCGGTATTGC                                         <  1:932441/35‑1
                                                                      CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATT   >  1:828223/1‑71
                                                                      CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATT   >  1:3483832/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATT   >  1:2242261/1‑68
                                                                         AGCATGAATCGCCTCACAATGAGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTC  <  1:160549/69‑1
                                                                                                                                              
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTC  >  W3110S.gb/2178182‑2178248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.