New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	4627241 =	77 (1.660)	3 (0.070)	3/96	NT	4.3%	coding (791/1332 nt)	yjjJ	predicted DNA‑binding transcriptional regulator
	?	W3110S.gb	4627263 =	61 (1.400)					coding (813/1332 nt)	yjjJ	predicted DNA‑binding transcriptional regulator
Rejected: Frequency below/above cutoff threshold.

AGCGACCATCGTTACCTTTGCAGTCAAAGCGTTCCGCTTCGAGGAATACCTGCCTGTTACTTGTTACAAGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4627312‑4627241 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATGGTCGCTTGCCTATTGTGTCGCTGGAG  >  W3110S.gb/4627263‑4627332                                                                                                                                                AGCGACCATCGTTACCTTTGCAGTCAAAGCGTTCCGCTTCGAGGAATACCTGCCTGTTACTTGTTACAAGC                                                                         <  1:4264175/71‑1                            AGCGTTCCGCTTCGAGGAATACCTGCCTGTTACTTGTTACAAGCA                                                                        <  1:1596775/45‑1                                                    GCCTGTTACTTGTTACAAGCAGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATG                            >  1:2478721/1‑65                                                      CTGTTACTTGTTACAAGCAGGTATTCCTCGAAGCGGAACGCTTT                                               <  1:1700286/44‑1                                                      CTGTTACTTGTTACAAGCAGGTATTCCTCGAAGAGGAACGC                                                  >  1:856391/1‑41                                                                      GCAGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATGGT                          >  1:1714180/1‑49                                                                       CAGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATGGTCGCTTGCCTATTGTGTCGCTG     >  1:683895/1‑69                                                                        AGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATGGTCGCTTGCCTATTGTGTCGCTGGAG  >  1:2493993/1‑71                                                                        AGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATGGTCGCTTGCCTATTGT            <  1:1756493/61‑1                                                                                                                                                AGCGACCATCGTTACCTTTGCAGTCAAAGCGTTCCGCTTCGAGGAATACCTGCCTGTTACTTGTTACAAGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/4627312‑4627241 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaGGTATTCCTCGAAGCGGAACGCTTTGACTGCAAAGGTAACGATGGTCGCTTGCCTATTGTGTCGCTGGAG  >  W3110S.gb/4627263‑4627332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.