Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 47.8% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT47.8% 10.5 / 96.4 67N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (16/19);  new base T (12/20);  total (28/39)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGAC  >  W3110S.gb/70367‑70501
                                                                   |                                                                   
aTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGcc                                                                  <  1:1984713/71‑1 (MQ=255)
 tggtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGcc                                                                  >  1:3746461/1‑70 (MQ=255)
   gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                               <  1:3900823/71‑1 (MQ=255)
   gtggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                               <  1:3896121/71‑1 (MQ=255)
    tggGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATc                                                              >  1:963097/1‑71 (MQ=255)
        aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          >  1:696838/1‑71 (MQ=255)
         gTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggtg                                                         >  1:1090787/1‑71 (MQ=255)
           aTGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGc                                                       >  1:3164807/1‑71 (MQ=255)
            tGATAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGccc                                                                 <  1:1937137/60‑1 (MQ=255)
            tGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATc                                                              <  1:558457/63‑1 (MQ=255)
              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAAc                                                                     >  1:3992567/1‑54 (MQ=255)
              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGc                                                       >  1:2975815/1‑68 (MQ=255)
              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCgg                                                     <  1:327420/70‑1 (MQ=255)
              aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCAGCCGGGATACTCGTTTAACGCCCATCtggtgg                                                        >  1:3497292/1‑67 (MQ=255)
               aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGc                                                                   <  1:3508032/55‑1 (MQ=255)
               aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                   <  1:3605953/71‑1 (MQ=255)
                 aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGtt                                                  >  1:2608020/1‑70 (MQ=255)
                  gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTa                                                <  1:3497266/71‑1 (MQ=255)
                   tATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                               <  1:4062804/71‑1 (MQ=255)
                   tATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                               >  1:774133/1‑71 (MQ=255)
                    aTGGCTGAAGCGCAAAATGATGCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAAc                                              >  1:11754/1‑71 (MQ=255)
                    aTGGCTGAAGCGCAAAATGATACCCTGCTGCCGGGATACTCGTTTACCGCCCATCTGGTGGCGGGTTTAAc                                              >  1:2951839/1‑71 (MQ=255)
                     tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGc                                                                   >  1:4031056/1‑49 (MQ=255)
                     tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAAc                                              <  1:1914860/70‑1 (MQ=255)
                       gCTGAAGCGCATAATGATCCCGTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:3412399/71‑1 (MQ=255)
                       gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:2698087/71‑1 (MQ=255)
                         tGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtg                                                            <  1:2298305/52‑1 (MQ=255)
                         tGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGTTTTAACGCCGa                                         >  1:1774761/1‑71 (MQ=255)
                         tGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                 <  1:218341/63‑1 (MQ=255)
                          gAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAt                                        >  1:3026933/1‑71 (MQ=255)
                           aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                       >  1:2143276/1‑71 (MQ=255)
                           aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGa                                         <  1:2755914/69‑1 (MQ=255)
                            aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                      <  1:1919016/71‑1 (MQ=255)
                            aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                      <  1:224171/71‑1 (MQ=255)
                                cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAAc                                              >  1:3894008/1‑59 (MQ=255)
                                cAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCt                                                             <  1:3454886/44‑1 (MQ=255)
                                  aaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGc                                                       >  1:1587913/1‑48 (MQ=255)
                                  aaaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCt                                                             >  1:1910828/1‑42 (MQ=255)
                                  aaaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAg                                    >  1:2489281/1‑67 (MQ=255)
                                       aTCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                      <  1:515692/60‑1 (MQ=255)
                                        tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                          <  1:2440542/71‑1 (MQ=255)
                                          cccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAg                                    <  1:2783826/59‑1 (MQ=255)
                                          cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                        <  1:3258240/71‑1 (MQ=255)
                                             tgctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                 <  1:791937/43‑1 (MQ=255)
                                             tgctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                      >  1:3863/1‑70 (MQ=255)
                                              gctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                               >  1:461009/1‑44 (MQ=255)
                                               ctgcCGGGATACTCGTTTAATGGCCATCTGGTGGc                                                       <  1:2778574/35‑1 (MQ=37)
                                               ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                   >  1:1195976/1‑71 (MQ=255)
                                               ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                   >  1:1388188/1‑71 (MQ=255)
                                                  ccGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                               >  1:2599361/1‑40 (MQ=255)
                                                     ggATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt             <  1:3253541/71‑1 (MQ=255)
                                                     ggATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt             <  1:2181751/71‑1 (MQ=255)
                                                      gATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:1336513/71‑1 (MQ=255)
                                                      gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:4125807/71‑1 (MQ=255)
                                                      gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:459414/71‑1 (MQ=255)
                                                      gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:1820680/71‑1 (MQ=255)
                                                       aTACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGACAACGGTTATCTCGATTTTTTTa           <  1:2110675/71‑1 (MQ=255)
                                                        tACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:1532101/69‑1 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa           <  1:32827/68‑1 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt          >  1:1674573/1‑69 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         <  1:1685471/70‑1 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         <  1:3289532/70‑1 (MQ=255)
                                                          cTCGTTTAATGCCCATCTGGGGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttt                <  1:3580164/63‑1 (MQ=255)
                                                           tCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt             <  1:1489106/65‑1 (MQ=255)
                                                           tCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAATGAGGCCAACGGTTATCTCGATTTTTTTATcga       >  1:1443013/1‑71 (MQ=255)
                                                               ttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAt                  <  1:308119/56‑1 (MQ=255)
                                                                tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:1360916/1‑71 (MQ=255)
                                                                 aaCGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtc                       >  1:3536461/1‑49 (MQ=255)
                                                                   |                                                                   
ATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGAC  >  W3110S.gb/70367‑70501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: