| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 155,463 | A→T | 100% | *247K (TAA→AAA) | ecpD ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 155,463 | 0 | A | T | 100.0% | 275.6 / NA | 93 | *247K (TAA→AAA) | ecpD | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (41/52); total (41/52) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAG > W3110S.gb/155396‑155528 | gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:1926018/1‑71 (MQ=255) atgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc > 1:2691026/1‑71 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:3498766/71‑1 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc < 1:3942974/71‑1 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc < 1:1339529/70‑1 (MQ=255) atAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt < 1:500813/70‑1 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg > 1:3938113/1‑71 (MQ=255) aaTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg > 1:2343506/1‑70 (MQ=255) aaTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg > 1:2552369/1‑69 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt < 1:1998896/67‑1 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:3606994/70‑1 (MQ=255) tttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt < 1:3279533/71‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:2789861/70‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:3339487/70‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:4204251/70‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:1933117/70‑1 (MQ=255) aGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt > 1:1491982/1‑61 (MQ=255) gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCtt < 1:585745/71‑1 (MQ=255) gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt < 1:3658011/70‑1 (MQ=255) gTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCt < 1:495260/70‑1 (MQ=255) tataTTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc < 1:974482/71‑1 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa > 1:2008346/1‑53 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:2959542/1‑71 (MQ=255) atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAcc < 1:720695/65‑1 (MQ=255) atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAcc < 1:4235578/65‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:1230917/71‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:554502/71‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:3207486/71‑1 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:1593883/1‑58 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:4150586/1‑58 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:515488/1‑58 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg > 1:3602366/1‑71 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:304383/59‑1 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg < 1:419268/70‑1 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg < 1:624065/70‑1 (MQ=255) taGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc > 1:445007/1‑70 (MQ=255) taGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc < 1:1484913/68‑1 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca > 1:3269153/1‑70 (MQ=255) cGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:671357/69‑1 (MQ=255) cGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca > 1:22050/1‑69 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg < 1:2595580/71‑1 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg < 1:3252481/71‑1 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg < 1:3247473/71‑1 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg > 1:2512103/1‑71 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCACCCTGGCAt > 1:2433942/1‑48 (MQ=38) cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:2076433/67‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc < 1:2373569/52‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:2430755/1‑71 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:3197199/1‑71 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:1692516/1‑71 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:3080722/1‑42 (MQ=255) gCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAACTTCAATTGCGCCACCAAAGTCAt > 1:3473748/1‑70 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc > 1:87051/1‑35 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGcca > 1:176932/1‑59 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:1527209/70‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:316538/70‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTa < 1:966103/71‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTAAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTa < 1:1166189/71‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt < 1:1309137/70‑1 (MQ=255) cTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg < 1:2032957/70‑1 (MQ=255) gCGGAATT‑TATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg > 1:2565075/1‑70 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg > 1:3756945/1‑70 (MQ=255) gAATTATATCCTTGCCTGATTTCAGCCTGGGATTACCTTCAATTGCGCCACCAAAGTCATTa < 1:2601861/62‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:2149242/71‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaa < 1:3944760/71‑1 (MQ=255) aTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa > 1:1675499/1‑70 (MQ=255) ttATATCCTTTCCTGATTTCAGCCTGGCATTACCTTc < 1:1404393/37‑1 (MQ=38) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacc < 1:3664381/49‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:2570109/39‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt > 1:2640621/1‑59 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCGACCAAAGTCATTAATGGCGTAAAAATg < 1:3494942/70‑1 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg > 1:198436/1‑70 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:1584536/35‑1 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:2034999/35‑1 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:995937/1‑50 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACt > 1:3072296/1‑71 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACt > 1:2003358/1‑71 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACt > 1:3067456/1‑71 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt < 1:4204971/71‑1 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt < 1:3119816/71‑1 (MQ=255) ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:3569052/50‑1 (MQ=255) ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:1355925/50‑1 (MQ=255) tGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa > 1:1817744/1‑51 (MQ=255) tGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa > 1:2716292/1‑51 (MQ=255) tGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa > 1:3563484/1‑51 (MQ=255) tGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt > 1:2156488/1‑69 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATgg > 1:2396614/1‑53 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:1162593/1‑71 (MQ=255) tGATTTCAGCCTGGCATTACCTTCAATTGCGccacc < 1:4017127/36‑1 (MQ=255) tGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt < 1:4175720/41‑1 (MQ=255) tGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt < 1:1590620/41‑1 (MQ=255) tGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTa > 1:3685414/1‑54 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg > 1:260000/1‑70 (MQ=255) | GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAG > W3110S.gb/155396‑155528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |