| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,101 | A→C | 53.5% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,101 | 0 | A | C | 53.5% | 144.9 / 157.7 | 146 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (33/35); new base C (45/33); total (78/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
CTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTG > W3110S.gb/236033‑236169 | cgcgtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTa < 1:243449‑M2/60‑1 (MQ=255) tGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAc > 1:239280/1‑71 (MQ=255) gcgtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACAGGCCAGATCGTTCTCGCTAc > 1:283862‑M2/11‑71 (MQ=255) tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAcc > 1:3035584/1‑70 (MQ=255) tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAcc < 1:4155398/70‑1 (MQ=255) aaaCGACAAGTCTGAGATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:2375268/70‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaa < 1:1705855/71‑1 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:167560/70‑1 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTa > 1:3269768‑M2/6‑65 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAccgca > 1:84422‑M2/6‑68 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAccgca < 1:3851497‑M2/65‑3 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1366635‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:209695‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1112107‑M2/6‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCTCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:1847908/1‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:1063403/1‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:520847/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2365073/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2554213/1‑71 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:2341911/1‑70 (MQ=255) tCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCgg < 1:1408901/69‑1 (MQ=255) tCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTa < 1:3809013/71‑1 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa > 1:2239092/1‑52 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGa < 1:2142388/70‑1 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:360545/1‑71 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1226518/1‑70 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAcc < 1:4053820/71‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:1973888/71‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1286816/1‑68 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:1793985/1‑67 (MQ=255) aagACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTAt < 1:1900233/60‑1 (MQ=255) tGACCGCTATGAGCTCTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:3005111/71‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg < 1:1896371/71‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:3017172/69‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTg < 1:664919/71‑1 (MQ=255) gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgc > 1:1825235/1‑71 (MQ=255) gCTATGAGCACTGCAATAACCCGCCAGATCGTTCTCGATACCGa < 1:2442933/44‑1 (MQ=38) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:710701/1‑45 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:3260674/1‑57 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacc < 1:678658/44‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:3982920/43‑1 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAaccac < 1:1644388/45‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCTCTACCGCAACCACCg < 1:2288696/46‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg < 1:1458234/46‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:1620798/1‑71 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa > 1:741316/1‑40 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa > 1:4231578/1‑40 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCgg > 1:1507265/1‑47 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:2390509/1‑71 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt > 1:4134932/1‑58 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2036713/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3263064/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3746791/70‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:2883076/1‑70 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:3586562/71‑1 (MQ=255) cTGCAATTACACGCCAGATCTTTCTCGCTACCGCAACCACCGCTATGAACCAGATTGGTGCGCACTATg > 1:1222860/1‑69 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:3778021/48‑1 (MQ=39) tGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1175565/68‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:2574201/1‑35 (MQ=38) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGGGCACTATGa < 1:1792154/68‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:1277665/68‑1 (MQ=255) cAATTACACTCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:3632208/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:85816/1‑47 (MQ=39) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:2517281/49‑1 (MQ=39) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:650545/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGAGCACTATGAAGGc > 1:2661233/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGAGCACTATGAAGGc > 1:4130371/1‑71 (MQ=255) aaTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg < 1:3484295/54‑1 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATaaa > 1:1191461/1‑66 (MQ=255) ttACACGCCATATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa < 1:394020/58‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:4016643/71‑1 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:3644913/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3304016/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:4235805/1‑57 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:3167327/1‑57 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:1015378/1‑65 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2135480/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2478864/1‑69 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:4081675/68‑1 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:1627731/71‑1 (MQ=255) acGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:3580955/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:476588/44‑1 (MQ=38) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACaa > 1:72655/1‑68 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:2181314/43‑1 (MQ=38) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2332990/1‑70 (MQ=255) gATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc < 1:4033696/58‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:880054/71‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:1744828/71‑1 (MQ=255) tCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:3593744/70‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:2123008/1‑45 (MQ=38) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:4200531/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga > 1:1492889/1‑69 (MQ=255) gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:3527093/71‑1 (MQ=255) ttCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:1330540/47‑1 (MQ=255) tctcGATACCGAAACCACCGGTATGAACCAGATTg < 1:2092692/35‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACaa > 1:389933/1‑56 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:940019/1‑71 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg > 1:4025790/1‑70 (MQ=255) cGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTg > 1:2162181/1‑71 (MQ=255) gATACCGAAACCACCGGTATCAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:2053177/56‑1 (MQ=255) | CTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTG > W3110S.gb/236033‑236169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |