| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,107 | A→C | 52.7% | E14A (GAA→GCA) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,107 | 0 | A | C | 52.7% | 120.3 / 146.8 | 131 | E14A (GAA→GCA) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (30/32); new base C (38/31); total (68/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
Only 100 of 148 total aligned reads displayed.
AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGAA > W3110S.gb/236038‑236177 | aaaCGACAAGTCTGAGATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:2375268/70‑1 (MQ=255)aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:167560/70‑1 (MQ=255)aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa < 1:2309652/70‑1 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:4216609‑M2/6‑71 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:1112107‑M2/6‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCTCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:1847908/1‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:1063403/1‑71 (MQ=255) aCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:520847/1‑71 (MQ=255) cGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAacc < 1:3709447/71‑1 (MQ=255) tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:1408072‑M2/2‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:3784812/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2554213/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2365073/1‑71 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:2341911/1‑70 (MQ=255) tCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCgg < 1:1408901/69‑1 (MQ=255) tCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTa < 1:3809013/71‑1 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:1585861/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:1799430/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGa < 1:2142388/70‑1 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:360545/1‑71 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGt < 1:2524266/62‑1 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:1793985/1‑67 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt < 1:2144416/71‑1 (MQ=255) tGACCGCTATGAGCTCTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:3005111/71‑1 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg < 1:1212345/62‑1 (MQ=255) gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCg > 1:1632635/1‑56 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTgg < 1:1896371/71‑1 (MQ=255) ccGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:3017172/69‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTg < 1:664919/71‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:4105316/1‑45 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:710701/1‑45 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:2468981/1‑48 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:3260674/1‑57 (MQ=255) aTGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaa < 1:2095802/42‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacc < 1:678658/44‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:3982920/43‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:1249825/71‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCTCTACCGCAACCACCg < 1:2288696/46‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg < 1:1458234/46‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa > 1:741316/1‑40 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCgg > 1:1507265/1‑47 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:2390509/1‑71 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:3194607/1‑71 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt > 1:4134932/1‑58 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2036713/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3267961/71‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:2883076/1‑70 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:3586562/71‑1 (MQ=255) cTGCAATTACACGCCAGATCTTTCTCGCTACCGCAACCACCGCTATGAACCAGATTGGTGCGCACTATg > 1:1222860/1‑69 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:3778021/48‑1 (MQ=39) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:2398632/67‑1 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1474286/68‑1 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1175565/68‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:2574201/1‑35 (MQ=38) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:1277665/68‑1 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:85816/1‑47 (MQ=39) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:650545/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGAGCACTATGAAGGc > 1:2661233/1‑71 (MQ=255) aaTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg < 1:3484295/54‑1 (MQ=255) aaTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAggg > 1:4020228/1‑69 (MQ=255) aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:876277/1‑71 (MQ=255) ttACACGCCATATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa < 1:394020/58‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:2126705/71‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:4016643/71‑1 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:3644913/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3304016/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:4235805/1‑57 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:1015378/1‑65 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2478864/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACAGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:1321769/1‑68 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:1905659/1‑68 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:1859774/68‑1 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:4081675/68‑1 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:1627731/71‑1 (MQ=255) acGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:3580955/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:476588/44‑1 (MQ=38) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACaa > 1:72655/1‑68 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:2181314/43‑1 (MQ=38) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg > 1:1757322/1‑66 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2332990/1‑70 (MQ=255) gATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc < 1:4033696/58‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:1744828/71‑1 (MQ=255) tCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:2044665/1‑52 (MQ=255) tCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:3593744/70‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa > 1:2123008/1‑45 (MQ=38) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:4200531/1‑67 (MQ=255) gTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt < 1:3527093/71‑1 (MQ=255) ttCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:1330540/47‑1 (MQ=255) tctcGATACCGAAACCACCGGTATGAACCAGATTg < 1:2092692/35‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:940019/1‑71 (MQ=255) cGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTg > 1:2162181/1‑71 (MQ=255) gctACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:4138517/54‑1 (MQ=255) gATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg < 1:4033759/68‑1 (MQ=255) aTACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3886351/42‑1 (MQ=255) tACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCg > 1:1159178/1‑71 (MQ=255) aCCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1962992/1‑57 (MQ=255) aCCGAAACCACCGGTATGAACCAGATTGGAGCGCAc > 1:957259/1‑36 (MQ=255) cGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg < 1:1649590/71‑1 (MQ=255) aaaCCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa > 1:3908424/1‑51 (MQ=255) aaaCCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg > 1:520441/1‑69 (MQ=255) | AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGAA > W3110S.gb/236038‑236177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |