| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 258,840 | A→G | 6.7% | V162A (GTT→GCT) | phoE ← | outer membrane phosphoporin protein E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 258,840 | 0 | A | G | 6.7% | 162.5 / 3.5 | 60 | V162A (GTT→GCT) | phoE | outer membrane phosphoporin protein E |
| Reads supporting (aligned to +/- strand): ref base A (20/36); new base G (2/2); total (22/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
TTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCT > W3110S.gb/258773‑258909 | ttGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACg > 1:230387/1‑70 (MQ=255)ttGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGc > 1:376662/1‑71 (MQ=255) tGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATaa > 1:4271730/1‑67 (MQ=255) cTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGcc < 1:587124/69‑1 (MQ=255) cTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCg < 1:123505/70‑1 (MQ=255) gTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCgg < 1:2634819/71‑1 (MQ=255) cgcgGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAGCGCCGaagaag > 1:1000412/1‑65 (MQ=255) cgcgGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGgtg > 1:1568231/1‑71 (MQ=255) gcgGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGgtgt > 1:3254357/1‑71 (MQ=255) cgGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCgaa < 1:2119046/59‑1 (MQ=255) gTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACg < 1:2588565/52‑1 (MQ=255) gTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACg < 1:2534614/52‑1 (MQ=255) gTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTcc > 1:3715385/1‑71 (MQ=255) ttttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAGCGCCga < 1:2690051/55‑1 (MQ=255) ttttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCgaagaa > 1:2599531/1‑59 (MQ=255) ttttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCg < 1:3131749/71‑1 (MQ=255) ttttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCAATCGATAACGCCGAAGAAGTCGGTGTTCCg < 1:1677286/71‑1 (MQ=255) tttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGa > 1:426187/1‑71 (MQ=255) ttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGa < 1:2323921/70‑1 (MQ=255) ttCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGa > 1:2618232/1‑70 (MQ=255) cGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATaa > 1:3577325/1‑45 (MQ=255) gTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACg > 1:1098605/1‑71 (MQ=255) tttttCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGt < 1:1601246/71‑1 (MQ=255) tttCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTc > 1:2278167/1‑70 (MQ=255) tttCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCg > 1:4065144/1‑71 (MQ=255) ttCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCgaag < 1:2075106/48‑1 (MQ=255) tCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGc < 1:348167/70‑1 (MQ=255) tCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGc > 1:3340796/1‑70 (MQ=255) ccTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCa < 1:3270080/70‑1 (MQ=255) cTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGa < 1:987498/71‑1 (MQ=255) ttGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGAc > 1:2660947/1‑71 (MQ=255) tGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGAcc > 1:1848636/1‑71 (MQ=255) gATATTGCAGGGTTAAGTTCAGTCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCg > 1:867549/1‑71 (MQ=255) atatTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCg < 1:1741215/70‑1 (MQ=255) tatTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCt < 1:2775899/71‑1 (MQ=255) tatTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCt < 1:3105611/71‑1 (MQ=255) ttGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTcc < 1:1547462/50‑1 (MQ=255) ttGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGcc < 1:1399461/61‑1 (MQ=255) cAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCAGATACGTCGCCAGACCGCTGgcg < 1:752136/70‑1 (MQ=255) gggTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGt > 1:1363006/1‑71 (MQ=255) ttAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATa < 1:2053550/46‑1 (MQ=255) ttAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATa < 1:1941705/46‑1 (MQ=255) aaGTTCAGGCCATCGATAGCGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTg > 1:736714/1‑69 (MQ=255) aaGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTgg > 1:3904167/1‑70 (MQ=255) gTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGAt < 1:3120031/41‑1 (MQ=255) ttCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTTGTCAt < 1:2186719/71‑1 (MQ=255) cAGGCCATCGATAGCGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATa < 1:4169959/70‑1 (MQ=255) cAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCAtt > 1:3142443/1‑69 (MQ=255) cAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATa < 1:42082/70‑1 (MQ=255) aGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATaa < 1:599733/70‑1 (MQ=255) aGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATaa < 1:2638379/70‑1 (MQ=255) aGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATaa < 1:3096537/70‑1 (MQ=255) ggCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATaaa < 1:2975654/70‑1 (MQ=255) gCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGt < 1:2338479/71‑1 (MQ=255) cATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGtt < 1:2560045/70‑1 (MQ=255) aTCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGt < 1:4281570/71‑1 (MQ=255) aTCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGt < 1:302432/71‑1 (MQ=255) aTCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTAGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGt < 1:2231305/71‑1 (MQ=255) gATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCgg > 1:94732/1‑71 (MQ=255) gATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCg < 1:1082537/70‑1 (MQ=255) aTAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAg < 1:830819/63‑1 (MQ=255) aTAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCgg > 1:167783/1‑70 (MQ=255) aCGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCt < 1:2711709/70‑1 (MQ=255) | TTGCTTTTTAACGTCGCGGTTTTCGTTTTTCCCTTGATATTGCAGGGTTAAGTTCAGGCCATCGATAACGCCGAAGAAGTCGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCT > W3110S.gb/258773‑258909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |