| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 267,502 | G→T | 7.7% | P228H (CCT→CAT) | ykfA ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 267,502 | 0 | G | T | 7.7% | 205.2 / 2.4 | 64 | P228H (CCT→CAT) | ykfA | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (29/30); new base T (3/2); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCC > W3110S.gb/267432‑267567 | gAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCC‑GACTACCGTCGTGCGAaaggaag < 1:1605796/70‑1 (MQ=255) cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg < 1:2909200/70‑1 (MQ=255) cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg > 1:2683482/1‑70 (MQ=255) ggCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATgg < 1:2280587/70‑1 (MQ=255) cccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGtt < 1:2443442/71‑1 (MQ=255) cccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGt > 1:1017009/1‑70 (MQ=255) cGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGc < 1:1065632/71‑1 (MQ=255) gACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCg < 1:2914986/71‑1 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGt > 1:1814010/1‑66 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:698285/1‑71 (MQ=255) aCGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:646287/1‑71 (MQ=255) ggTTTCACAAAAATCGCTACGAGCCTGTTCCCGGACTACAGTCGTGCGAAAGGAAGGATGGAGTTGCg > 1:182826/1‑68 (MQ=255) gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGacac < 1:3632019/71‑1 (MQ=255) ttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcacca > 1:4053378/1‑71 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg < 1:515549/56‑1 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:2690516/1‑65 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGa > 1:405412/1‑65 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcaccac > 1:3527152/1‑71 (MQ=255) tCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcaccac > 1:942932/1‑71 (MQ=255) cACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcaccac > 1:3635463/1‑70 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGa < 1:1346177/56‑1 (MQ=255) ccAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCg > 1:1494102/1‑70 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAg < 1:1495723/55‑1 (MQ=255) cAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCggg > 1:2768509/1‑71 (MQ=255) aaaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAaacgaagg > 1:3104077/1‑48 (MQ=255) aaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAg > 1:2361905/1‑53 (MQ=255) aaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCt > 1:1485415/1‑71 (MQ=255) aaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCt > 1:2624306/1‑71 (MQ=255) aaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCt < 1:3241527/71‑1 (MQ=255) aaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCt > 1:3408327/1‑71 (MQ=255) aaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:3968995/71‑1 (MQ=255) aTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCggg > 1:2028930/1‑67 (MQ=255) aTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtgg < 1:3220723/71‑1 (MQ=255) cTACGAGCCTGTTCCCGGACTACCGTCGTGCGAaaggaagg < 1:3867329/41‑1 (MQ=255) cTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg < 1:2725677/44‑1 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCg > 1:506166/1‑49 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt < 1:417358/70‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc < 1:3433648/71‑1 (MQ=255) tGTTCCCGGACTACCGTCGTGCGAAAGGAAGTATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTgg > 1:758466/1‑71 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc > 1:2874163/1‑66 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggtg < 1:989677/59‑1 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggtg < 1:2338295/59‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggca < 1:3486187/71‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc < 1:3351901/70‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc < 1:2000545/70‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc < 1:2879343/70‑1 (MQ=255) tCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt > 1:690878/1‑61 (MQ=255) ccGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcag < 1:471849/69‑1 (MQ=255) cGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcaggca > 1:2739415/1‑71 (MQ=255) ggACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggcaggca > 1:126650/1‑70 (MQ=255) ggACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCAc > 1:4205238/1‑71 (MQ=255) gACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc < 1:2119007/64‑1 (MQ=255) gACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACt < 1:295788/71‑1 (MQ=255) aCTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt < 1:300689/71‑1 (MQ=255) cTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa < 1:1749605/71‑1 (MQ=255) tACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACg > 1:3739390/1‑57 (MQ=255) tACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACtt < 1:2627816/69‑1 (MQ=255) ccGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCt < 1:1874479/51‑1 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCAc > 1:2470061/1‑65 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTaa < 1:1202146/69‑1 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAat < 1:2829296/70‑1 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAat < 1:3657460/70‑1 (MQ=255) tcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtggt > 1:77676/1‑43 (MQ=255) gtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg > 1:4017324/1‑39 (MQ=255) cGAAAGGAAGGATGGAGTTGCGACACCACCGGGCtg < 1:963877/36‑1 (MQ=255) gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCg > 1:3280822/1‑71 (MQ=255) aaaGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggc > 1:2527744/1‑49 (MQ=255) aaggaatgATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGc > 1:2401154/1‑71 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGcc < 1:2763402/71‑1 (MQ=255) aggaaggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGcc < 1:3900597/71‑1 (MQ=255) | GAGTACGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCC > W3110S.gb/267432‑267567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |