| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 345,099 | C→T | 9.6% | A155T (GCC→ACC) | yahN ← | neutral amino‑acid efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 345,099 | 0 | C | T | 9.6% | 156.9 / 6.4 | 52 | A155T (GCC→ACC) | yahN | neutral amino‑acid efflux system |
| Reads supporting (aligned to +/- strand): ref base C (25/22); new base T (2/3); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
AGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTA > W3110S.gb/345036‑345167 | aGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAAt > 1:2880111/1‑71 (MQ=255) gATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAt > 1:3231888/1‑65 (MQ=255) gATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATg > 1:2392713/1‑71 (MQ=255) aTAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGt < 1:4113673/71‑1 (MQ=255) tAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGtt < 1:3039580/71‑1 (MQ=255) aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:4262694/1‑71 (MQ=255) aaTTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:2294148/1‑71 (MQ=255) gATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACt > 1:804152/1‑69 (MQ=255) gCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATg > 1:1374524/1‑61 (MQ=255) cGAGCACAATCCCCGGCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaa > 1:2481156/1‑70 (MQ=255) cGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCAt > 1:2041692/1‑54 (MQ=255) gAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGaaaa < 1:2129334/71‑1 (MQ=255) aGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa < 1:3895902/70‑1 (MQ=255) gCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATa > 1:3056279/1‑71 (MQ=255) acaATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACt < 1:621890/71‑1 (MQ=255) aTCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGtt < 1:4062115/54‑1 (MQ=255) cGCCAAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATaaaaa < 1:1101450/71‑1 (MQ=255) gCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATATAAAGTTACTGAGAAAATAc > 1:262517/1‑61 (MQ=255) cccAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATa > 1:3103001/1‑59 (MQ=255) cccAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAAt < 1:3057225/71‑1 (MQ=255) cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTa < 1:1431125/40‑1 (MQ=255) cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaa < 1:2240190/71‑1 (MQ=255) cAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaa < 1:1659557/71‑1 (MQ=255) ccATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATaaaaaa > 1:607402/1‑64 (MQ=255) ccATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATaaaaaa > 1:813179/1‑64 (MQ=255) cATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACg > 1:939924/1‑70 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTgag < 1:856777/44‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa < 1:1325513/48‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaa < 1:2500948/46‑1 (MQ=255) ttAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGtt < 1:4073627/71‑1 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGTATTTAATGTTACTGAGAAAATACTGa > 1:418995/1‑52 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:1152013/1‑37 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:499203/1‑37 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTa > 1:197159/1‑37 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaa > 1:3845383/1‑45 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATa > 1:1703471/1‑48 (MQ=255) aaaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTg > 1:905027/1‑71 (MQ=255) aaCGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGc > 1:2690491/1‑71 (MQ=255) ccATGTTGGTGTTTCGGCATTTAATGTTACTGAGaaaa < 1:450591/38‑1 (MQ=255) ccATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTTCGGGTTa < 1:1715997/70‑1 (MQ=255) aTGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAAt < 1:2949946/50‑1 (MQ=255) aTGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAAt < 1:2932539/50‑1 (MQ=255) aTGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTagag < 1:408347/71‑1 (MQ=255) tGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaaaa > 1:3978929/1‑53 (MQ=255) tGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATaaaa > 1:1377087/1‑53 (MQ=255) tGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCg < 1:3627559/62‑1 (MQ=255) tGGTGTTTCGGCATTTAATGTTACTGAGAAAATAc > 1:1105675/1‑35 (MQ=255) ggTGTTTCGGTATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGAt < 1:322381/70‑1 (MQ=255) tttCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTaga < 1:4004250/61‑1 (MQ=255) ttCGGCATTTAATGTTACTGAGAAAATACTGATaaaa < 1:4252383/37‑1 (MQ=255) tCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTa > 1:286790/1‑57 (MQ=255) ggCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGtaatta < 1:2365247/71‑1 (MQ=255) cATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGt < 1:384467/64‑1 (MQ=255) | AGATAATTGATGCGAGCACAATCCCCGCCCAGGCCATTAAACGTGCCCATGTTGGTGTTTCGGCATTTAATGTTACTGAGAAAATACTGATAAAAAATAAAACGGTTTGCGGGTTAGAGAGATCGGTAATTA > W3110S.gb/345036‑345167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |