| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 349,140 | C→T | 11.1% | intergenic (+344/‑96) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 349,140 | 0 | C | T | 11.1% | 125.6 / 6.7 | 45 | intergenic (+344/‑96) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base C (28/12); new base T (2/3); total (30/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01 | |||||||||||
CGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTAC > W3110S.gb/349081‑349210 | cGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa < 1:2631785/71‑1 (MQ=32)cGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa < 1:1740045/71‑1 (MQ=32) aTAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaactg < 1:3900272/71‑1 (MQ=34) aGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa > 1:2682017/1‑66 (MQ=33) gCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGaactgaactg > 1:122120/1‑68 (MQ=35) gCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAg > 1:3741411/1‑71 (MQ=34) cATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGTGtgaa < 1:3183506/59‑1 (MQ=16) tCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTc < 1:500653/71‑1 (MQ=34) ccGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGtgaa > 1:1905004/1‑56 (MQ=33) cAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGAt > 1:1988228/1‑70 (MQ=35) aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGAt > 1:4006576/1‑69 (MQ=35) aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATaa < 1:2963751/71‑1 (MQ=34) aaCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATaa < 1:2490333/71‑1 (MQ=34) aCCGATGCCTGATGCGCCTCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAg < 1:1599134/71‑1 (MQ=255) cGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGAc > 1:3823205/1‑71 (MQ=34) aTGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATa > 1:3242136/1‑65 (MQ=25) aTGCCTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGc < 1:1911281/71‑1 (MQ=21) gCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCa > 1:4081717/1‑70 (MQ=35) ccTGATGCGCCGCTGACGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCAt < 1:3818646/70‑1 (MQ=25) ccTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATa < 1:4099595/71‑1 (MQ=34) gATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAg > 1:2176453/1‑61 (MQ=35) aTGCGCCGCTGACGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGc < 1:1942846/69‑1 (MQ=25) aTGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGt > 1:4202901/1‑71 (MQ=34) cgccgcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAg > 1:756619/1‑47 (MQ=38) cgccgcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCg > 1:741681/1‑70 (MQ=35) gacgcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc > 1:272640/3‑70 (MQ=25) gcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc > 1:3002997/1‑71 (MQ=34) gcTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc > 1:2510620/1‑71 (MQ=34) cTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc > 1:1623212/1‑70 (MQ=35) gACGCGACTTATCAGGCCTACGGGGTgaactgaact < 1:3703002/36‑1 (MQ=40) gACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa > 1:3629806/1‑70 (MQ=35) aCGCGACTTATCAGGCTTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGac > 1:4199158/1‑70 (MQ=25) aCGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTc < 1:1895830/42‑1 (MQ=38) aCGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGac < 1:1179856/70‑1 (MQ=35) gcgACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGacaa > 1:2699203/1‑70 (MQ=35) cgACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGa > 1:1316328/1‑47 (MQ=38) gACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAcaacaa > 1:4242261/1‑71 (MQ=38) ttATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtct > 1:1696580/1‑71 (MQ=255) aTCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc > 1:401922/1‑70 (MQ=255) aTCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAAtctc > 1:796012/1‑70 (MQ=255) tCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTc > 1:1630122/1‑51 (MQ=35) cAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGa > 1:4029330/1‑70 (MQ=255) aGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAcc > 1:25972/1‑71 (MQ=255) aGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAc > 1:1307964/1‑70 (MQ=255) aGGCCTACGAGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGAcc > 1:1109138/1‑71 (MQ=255) cTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTAc < 1:300604/71‑1 (MQ=255) | CGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAATCTCGACCCTAC > W3110S.gb/349081‑349210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |