| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 429,850 | T→C | 12.8% | Y8H (TAT→CAT) | yajD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 429,850 | 0 | T | C | 12.8% | 116.6 / 10.6 | 47 | Y8H (TAT→CAT) | yajD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/21); new base C (3/3); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGT > W3110S.gb/429781‑429918 | aTCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTa < 1:825594/71‑1 (MQ=255) ttGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAGAAAACCATgc > 1:3606780/1‑71 (MQ=255) ttGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgc > 1:3591787/1‑71 (MQ=255) aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTa < 1:2226495/71‑1 (MQ=255) tGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACt > 1:3154111/1‑58 (MQ=255) gcgcGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGa > 1:971546/1‑70 (MQ=255) cgcgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaaa < 1:3673827/71‑1 (MQ=255) cgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGc < 1:1222542/71‑1 (MQ=255) tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt > 1:2012115/1‑48 (MQ=255) tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTCTgc < 1:3755596/46‑1 (MQ=255) tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa < 1:3464396/71‑1 (MQ=255) tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGa < 1:3406462/70‑1 (MQ=255) ttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaa > 1:2183843/1‑70 (MQ=255) tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgcga > 1:4232701/1‑45 (MQ=255) tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaa > 1:1816327/1‑70 (MQ=255) cATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGaa < 1:672156/53‑1 (MQ=255) cAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaa > 1:1969374/1‑49 (MQ=255) cAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCAt > 1:2698364/1‑71 (MQ=255) ggTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa < 1:77216/71‑1 (MQ=255) aCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:733302/70‑1 (MQ=255) cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAg < 1:1363402/40‑1 (MQ=255) ttttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAg < 1:174498/39‑1 (MQ=255) tttCATGGCTATCATCCCAAAAAACCATGCGCGGTTa > 1:2197346/1‑37 (MQ=255) tttCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATc > 1:2459062/1‑70 (MQ=255) ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTa > 1:12301/1‑71 (MQ=255) ggCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa > 1:2190100/1‑61 (MQ=255) gCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAAt > 1:354154/1‑62 (MQ=255) gCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt < 1:697617/70‑1 (MQ=255) cTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTgg < 1:2700958/71‑1 (MQ=255) tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa < 1:3815336/58‑1 (MQ=255) tcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt < 1:3835020/66‑1 (MQ=255) tcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGt > 1:54412/1‑70 (MQ=255) tcatcCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAg < 1:2290532/48‑1 (MQ=255) catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGaaaa > 1:3637423/1‑45 (MQ=255) atcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaa > 1:2815250/1‑52 (MQ=255) atcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAAt > 1:334933/1‑56 (MQ=255) ccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCgg > 1:1270309/1‑71 (MQ=255) aaaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATc < 1:2230464/52‑1 (MQ=255) aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAg < 1:3421931/40‑1 (MQ=255) aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCg < 1:3969514/71‑1 (MQ=255) aaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGc > 1:3414034/1‑71 (MQ=255) aaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTaaaa > 1:1527694/1‑46 (MQ=255) aaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTg < 1:1010578/71‑1 (MQ=255) aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGt < 1:3588822/71‑1 (MQ=255) aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGt < 1:1276157/71‑1 (MQ=255) aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGt < 1:216365/71‑1 (MQ=255) aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGt > 1:1787017/1‑71 (MQ=255) cTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCt > 1:2937988/1‑59 (MQ=255) | ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGT > W3110S.gb/429781‑429918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |