| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 478,343 | C→T | 8.3% | A45T (GCT→ACT) | ylaC ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 478,343 | 0 | C | T | 8.3% | 187.7 / 6.1 | 60 | A45T (GCT→ACT) | ylaC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (34/21); new base T (2/3); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTC > W3110S.gb/478274‑478412 | cAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCa < 1:1346048/71‑1 (MQ=255)cAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCa < 1:2703620/71‑1 (MQ=255) ccAGTAGCCAGACAGAGCCTGACAGCGTTTCGGAGTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACg < 1:3196038/71‑1 (MQ=255) cAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACg > 1:217229/1‑70 (MQ=255) aGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTa < 1:2987740/71‑1 (MQ=255) gTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTa > 1:2736271/1‑70 (MQ=255) gCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACAt < 1:1247481/70‑1 (MQ=255) gCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACAt < 1:4103873/70‑1 (MQ=255) gCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATa > 1:3319629/1‑71 (MQ=255) gCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATa > 1:3622494/1‑71 (MQ=255) agacagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATAcc > 1:1488887/1‑70 (MQ=255) acagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa < 1:206402/64‑1 (MQ=255) acagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGAt < 1:1843037/71‑1 (MQ=255) agagCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGTCAAAAAAAGCAACGTACa > 1:2494128/1‑62 (MQ=255) agagCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATAcc < 1:934022/66‑1 (MQ=255) ccTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAg < 1:323340/71‑1 (MQ=255) cTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGc < 1:961990/71‑1 (MQ=255) tGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc < 1:1587988/48‑1 (MQ=255) tGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGc < 1:3836263/48‑1 (MQ=255) cAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCg > 1:1991244/1‑71 (MQ=255) cAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCg < 1:2652/71‑1 (MQ=255) cAGCGTTTCGGACTGCAAAATCACCGCCTGGGTGGCAAAAAAAGCAACGTACATACCGATAAACTGCCCCg < 1:555620/71‑1 (MQ=255) aGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCg > 1:931900/1‑70 (MQ=255) gCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGa > 1:617688/1‑71 (MQ=255) cGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGa > 1:2859978/1‑70 (MQ=255) gTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATg > 1:3756053/1‑71 (MQ=255) ttCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGtt > 1:2880017/1‑71 (MQ=255) tCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACa > 1:4163599/1‑46 (MQ=255) tCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGcc > 1:3128933/1‑61 (MQ=255) tCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGtt < 1:4217610/70‑1 (MQ=255) cGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTa > 1:1498332/1‑71 (MQ=255) ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTAc < 1:691353/71‑1 (MQ=255) gACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTAc > 1:813604/1‑42 (MQ=255) cTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa > 1:2993474/1‑71 (MQ=255) tGCAACATCACCGCCAGGGTTGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGa > 1:681754/1‑70 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATa > 1:2651141/1‑70 (MQ=255) aaCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACATCCGGGGCTGTTTatc > 1:3435621/1‑63 (MQ=37) aaCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaa > 1:2227296/1‑70 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaa > 1:3327130/1‑46 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa < 1:464730/71‑1 (MQ=255) cATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAAc > 1:3543271/1‑71 (MQ=255) aTCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTa > 1:1151704/1‑60 (MQ=255) cACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaa > 1:3680553/1‑42 (MQ=255) cACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGa > 1:1988976/1‑71 (MQ=255) gCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATAc > 1:2713918/1‑70 (MQ=255) cAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTa > 1:840914/1‑70 (MQ=255) aGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGt > 1:1727033/1‑48 (MQ=255) cgCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGTATaaaa < 1:4058001/54‑1 (MQ=255) gCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCgg < 1:753756/69‑1 (MQ=255) cAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaa < 1:1865755/51‑1 (MQ=255) aaaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAg > 1:2938505/1‑64 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGtt < 1:1731209/70‑1 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTa > 1:2381990/1‑41 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa > 1:3811102/1‑50 (MQ=255) aaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttg > 1:1835901/1‑71 (MQ=255) aaaaGCAACGTACATACCGATAAACAGCCCCGGATGtt > 1:2452989/1‑38 (MQ=255) aaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATa < 1:255442/46‑1 (MQ=255) aaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACg > 1:670705/1‑41 (MQ=255) aaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACg > 1:1439207/1‑41 (MQ=255) aaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAg < 1:2389742/59‑1 (MQ=255) aaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTttgttg < 1:2946884/71‑1 (MQ=255) gCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTc < 1:4045325/71‑1 (MQ=255) | CAACCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGTTTGTTGTC > W3110S.gb/478274‑478412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |