| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 38.7% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 38.7% | 50.8 / 68.2 | 62 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (14/24); new base T (14/10); total (28/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGC > W3110S.gb/70513‑70649 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACTAGAATTTGTCTg > 1:3771073/1‑71 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:3408157/1‑71 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:3918607/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:2655595/1‑71 (MQ=255) ttCTCAATCTAACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGc > 1:693908/1‑62 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTg < 1:96995/70‑1 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt < 1:373286/57‑1 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa < 1:3739917/68‑1 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGAc > 1:2915726/1‑57 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGa < 1:3659309/64‑1 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt > 1:3394922/1‑70 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt < 1:3005996/68‑1 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTg > 1:1672689/1‑71 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTg > 1:3579759/1‑71 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGatat < 1:3283695/62‑1 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:3746366/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCg < 1:1368701/47‑1 (MQ=255) cgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc < 1:892127/48‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:2813294/71‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata < 1:3070857/53‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1175542/71‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:2991571/71‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:2996755/71‑1 (MQ=255) ggtggtGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga < 1:3095416/71‑1 (MQ=255) ttgtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGtt < 1:3994298/53‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt < 1:1021563/50‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:2533913/71‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:4149045/1‑71 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:1358484/70‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2172456/58‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:3282660/71‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:3499029/1‑70 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:2058195/1‑71 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:1848369/1‑71 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt < 1:2266550/45‑1 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCAGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:3948198/1‑70 (MQ=255) aaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGt > 1:1482684/1‑47 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt > 1:1406017/1‑46 (MQ=255) aaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTc < 1:24834/48‑1 (MQ=255) aaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa < 1:3293641/71‑1 (MQ=255) aTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:3535723/1‑64 (MQ=255) cAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:109911/1‑60 (MQ=255) cAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg > 1:458387/1‑71 (MQ=255) aGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgt > 1:3674534/1‑71 (MQ=255) ggACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcg < 1:3423075/71‑1 (MQ=255) gACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt < 1:3207511/71‑1 (MQ=255) aCGAGAATTTGTCTGCCGACCGGGTGATATTTTGCt > 1:679348/1‑36 (MQ=255) aCGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGt < 1:413548/38‑1 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:434064/71‑1 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGtt < 1:794710/37‑1 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:1407273/1‑71 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:3584448/1‑71 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:1163863/41‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc > 1:1779275/1‑71 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc > 1:3530876/1‑71 (MQ=255) gaATTTGTTTGCCGACCGGGGGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATcc < 1:4201628/71‑1 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:2516765/52‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt < 1:3357284/48‑1 (MQ=255) aTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCgg < 1:2015732/71‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCa > 1:2485516/1‑39 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:4180063/1‑69 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa > 1:2218928/1‑37 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:2124478/1‑38 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc > 1:1079862/1‑65 (MQ=255) tGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa > 1:1722826/1‑37 (MQ=255) tCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGc < 1:4282430/70‑1 (MQ=255) | AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGC > W3110S.gb/70513‑70649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |