| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 647,863 | A→G | 9.1% | M311T (ATG→ACG) | citF ← | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 647,863 | 0 | A | G | 9.1% | 116.0 / 4.7 | 44 | M311T (ATG→ACG) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base A (17/23); new base G (2/2); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGA > W3110S.gb/647794‑647932 | gcagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGTTCAACCAt < 1:3784602/71‑1 (MQ=255)gcagcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAt > 1:2285424/1‑71 (MQ=255) agcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcg > 1:3305938/1‑71 (MQ=255) agcaTGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcg > 1:1666418/1‑71 (MQ=255) gCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg < 1:604675/71‑1 (MQ=255) cTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt < 1:2164206/71‑1 (MQ=255) tGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGt > 1:2039768/1‑70 (MQ=255) tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa > 1:720967/1‑70 (MQ=255) tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa > 1:909119/1‑70 (MQ=255) tCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTaa < 1:1287654/70‑1 (MQ=255) aaaGCTCTGCAGATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTa > 1:1665193/1‑67 (MQ=255) ctctGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGTAATACCGcc < 1:608713/71‑1 (MQ=255) tGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAg > 1:2992163/1‑71 (MQ=255) gCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGcc < 1:2868206/67‑1 (MQ=255) cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa < 1:652245/68‑1 (MQ=255) cacaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCaa < 1:734307/68‑1 (MQ=255) acaTCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGc < 1:3648255/71‑1 (MQ=255) tCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATAcc < 1:1146306/59‑1 (MQ=255) ccAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtc > 1:2599201/1‑44 (MQ=255) agcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAggg > 1:4079433/1‑64 (MQ=255) gcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgt < 1:117714/43‑1 (MQ=255) gcagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCg > 1:1204566/1‑71 (MQ=255) cagTTTGCGGATCAGACTTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCgg < 1:52426/71‑1 (MQ=255) cagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCAtcgt < 1:934285/42‑1 (MQ=255) cagTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCgg > 1:2099627/1‑47 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:3185145/1‑70 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg > 1:311483/1‑70 (MQ=255) tGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGcgcg < 1:391719/70‑1 (MQ=255) gATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGaa < 1:2084509/57‑1 (MQ=255) aTCAGACCTTTTTCGTGCAGGTCAACCGTCGTCGCGGt < 1:1230511/38‑1 (MQ=255) aTCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATc < 1:3694905/71‑1 (MQ=255) tCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATcg < 1:2823518/71‑1 (MQ=255) cAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAAt < 1:3226880/39‑1 (MQ=255) cTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTa < 1:1323471/71‑1 (MQ=255) ttttCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGaa > 1:1599675/1‑58 (MQ=255) tttCGTGCAGGTCAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACg > 1:3318870/1‑70 (MQ=255) gTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCAtt < 1:642915/70‑1 (MQ=255) cAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTg < 1:1961871/70‑1 (MQ=255) tCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc < 1:379573/71‑1 (MQ=255) cAACCGTCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:1449713/1‑70 (MQ=255) aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGTCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:3626529/1‑69 (MQ=255) aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTcc < 1:1550680/70‑1 (MQ=255) aaCCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTc > 1:272064/1‑69 (MQ=255) cATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGa < 1:3889959/71‑1 (MQ=255) | GCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAGACCTTTTTCGTGCAGGTCAACCATCGTCGCGGTAATACCGCCAAGGGCGAAGTCGGCGCGAATATCGCGGCTACGCATTTTGTCTTCCAGGA > W3110S.gb/647794‑647932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |