| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,008,134 | C→T | 15.1% | intergenic (‑112/‑132) | ycbX ← / → ycbY | predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,008,134 | 0 | C | T | 15.1% | 139.9 / 15.3 | 53 | intergenic (‑112/‑132) | ycbX/ycbY | predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (20/25); new base T (4/4); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
ACGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCGCT > W3110S.gb/1008067‑1008198 | acGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGt < 1:2536556/71‑1 (MQ=255) gcgcTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGttt < 1:2853959/71‑1 (MQ=255) taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTgg > 1:4074484/1‑71 (MQ=255) taGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTgg > 1:4079071/1‑71 (MQ=255) aGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTggg > 1:2412703/1‑71 (MQ=255) cTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAg < 1:3137448/57‑1 (MQ=255) tataATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTa > 1:1663930/1‑71 (MQ=255) taATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTGTTTGTCAGTTTGGCCCTGGGTTATg < 1:910476/71‑1 (MQ=255) taATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGt < 1:206495/67‑1 (MQ=255) taATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATg < 1:1845269/71‑1 (MQ=255) taATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATg > 1:589363/1‑71 (MQ=255) aTGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCa > 1:1786977/1‑51 (MQ=255) caacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaaa < 1:169518/71‑1 (MQ=255) caacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaaa < 1:4238916/71‑1 (MQ=255) aacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGaaa < 1:2016461/70‑1 (MQ=255) aacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGa < 1:4243730/68‑1 (MQ=255) caaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAAt > 1:881297/1‑69 (MQ=255) caaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATcc < 1:3373586/71‑1 (MQ=255) aaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAg < 1:3228364/43‑1 (MQ=255) aaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCa > 1:3901951/1‑42 (MQ=255) aaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTAt > 1:2377698/1‑59 (MQ=255) ttttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTc < 1:3768390/70‑1 (MQ=255) ttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGtt > 1:998332/1‑41 (MQ=255) ttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATTCTCGAAATCCTCACg > 1:3142308/1‑71 (MQ=255) ttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATTCTCGAAATCCTCACg > 1:3147411/1‑71 (MQ=255) ttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCAc < 1:211070/70‑1 (MQ=255) ttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACg > 1:1998247/1‑71 (MQ=255) tCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACg < 1:614608/70‑1 (MQ=255) cTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt < 1:3365347/70‑1 (MQ=255) cTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCT‑‑GTTATGCTCGAAATCCTCACGTAc < 1:1869678/70‑1 (MQ=37) aGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTa > 1:1348556/1‑49 (MQ=255) aGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTa < 1:1141926/68‑1 (MQ=255) ccTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTgtgt < 1:606611/71‑1 (MQ=255) cTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCg < 1:563097/50‑1 (MQ=255) cTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTa < 1:1546414/71‑1 (MQ=255) tGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTg > 1:1403306/1‑38 (MQ=255) tCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTc > 1:3787545/1‑55 (MQ=255) aTTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTtgtg < 1:4047925/62‑1 (MQ=255) tAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGt > 1:4000682/1‑71 (MQ=255) aGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGtt > 1:3779632/1‑71 (MQ=255) ggCTATTTTATTTGTCAGTTTGGCCCTGGGTTATg > 1:3749783/1‑35 (MQ=255) gCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTGCg < 1:4003674/66‑1 (MQ=255) gCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTtc < 1:3389704/71‑1 (MQ=255) cTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGaa < 1:3680124/39‑1 (MQ=255) tattttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGt > 1:2650613/1‑48 (MQ=255) tattttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTa > 1:253207/1‑49 (MQ=255) tattttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTtctc > 1:1441192/1‑71 (MQ=255) tttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGtt < 1:501711/64‑1 (MQ=255) ttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGt < 1:986036/62‑1 (MQ=255) ttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgcg > 1:1589989/1‑71 (MQ=255) tatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGttt > 1:4033242/1‑63 (MQ=255) tttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTc < 1:2491438/37‑1 (MQ=255) tttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCGCt < 1:2545939/70‑1 (MQ=255) cAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTa > 1:4035113/1‑45 (MQ=255) | ACGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCGCT > W3110S.gb/1008067‑1008198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |