| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,036,407 | A→G | 10.2% | E72E (GAA→GAG) | hyaD → | protein involved in processing of HyaA and HyaB proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,036,407 | 0 | A | G | 10.2% | 94.8 / 4.8 | 39 | E72E (GAA→GAG) | hyaD | protein involved in processing of HyaA and HyaB proteins |
| Reads supporting (aligned to +/- strand): ref base A (21/14); new base G (2/2); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01 | |||||||||||
AACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTG > W3110S.gb/1036339‑1036476 | aaCTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggaacc > 1:434328/1‑71 (MQ=255) cTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggaacctggaa < 1:1685055/70‑1 (MQ=255) gggTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggaacc > 1:1261979/1‑62 (MQ=255) gggTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACg < 1:3233774/69‑1 (MQ=255) tGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGaa > 1:2634630/1‑71 (MQ=255) tCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggaacctgga > 1:1194811/1‑59 (MQ=255) tCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTg > 1:3422021/1‑65 (MQ=255) tCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc < 1:1012444/71‑1 (MQ=255) cGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc > 1:3605299/1‑70 (MQ=255) gAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTa > 1:982732/1‑71 (MQ=255) aaaGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTAt > 1:3592295/1‑71 (MQ=255) aaaGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTAt > 1:1655346/1‑71 (MQ=255) aGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCt > 1:4167758/1‑59 (MQ=255) aGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGc > 1:280878/1‑71 (MQ=255) aGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGc > 1:374201/1‑71 (MQ=255) ccagccaTCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAc < 1:1974383/61‑1 (MQ=255) ccagccaTCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg < 1:4006113/70‑1 (MQ=255) cagccaTCTGTTGATTCTCGATTCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg < 1:953967/69‑1 (MQ=255) cagccaTCTGTTGATTCTCGATGCCATTGACTACGGGctggaacctggaa > 1:2680078/1‑50 (MQ=255) ccaTCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGGCGGAGaa < 1:1535797/70‑1 (MQ=255) caTCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACg < 1:2181393/71‑1 (MQ=255) tCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGc > 1:2188904/1‑47 (MQ=255) tCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGgaga < 1:2531899/66‑1 (MQ=255) tGTTGATTCTCGATGCCATTGACTACGGGCAGGAGCCTGGAACGCTGCGAACCTATGCCGGAGAACGCAtt > 1:138675/1‑71 (MQ=255) ttGATTCTCGATGCCATTGACTACGGGctggaacctg < 1:3300689/37‑1 (MQ=255) ttGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc < 1:2460463/70‑1 (MQ=255) tGATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGa < 1:2560776/47‑1 (MQ=255) gATTCTCGATGCCATTGACTACGGGCTGGAGCCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCgg > 1:3588389/1‑71 (MQ=255) gATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGc > 1:3643230/1‑44 (MQ=255) gATTCTAGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCgg > 1:551198/1‑71 (MQ=255) aTTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCgg < 1:64369/70‑1 (MQ=255) tcGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAt > 1:2897923/1‑71 (MQ=255) cGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc > 1:3769536/1‑71 (MQ=255) gATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGaa > 1:626867/1‑54 (MQ=255) ccATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGga < 1:4127065/47‑1 (MQ=255) ttGACTACGGGCTGGAACCTGGAACGCTGCGAAcc < 1:3802217/35‑1 (MQ=255) ttGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGc > 1:1571634/1‑40 (MQ=255) ttGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGc > 1:1010579/1‑40 (MQ=255) ttGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCg > 1:3594000/1‑56 (MQ=255) gACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTc > 1:611700/1‑52 (MQ=255) aCTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGcgcg > 1:3952530/1‑68 (MQ=255) cTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCt > 1:3729583/1‑55 (MQ=255) cGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGaaa < 1:2573922/70‑1 (MQ=255) gggCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAAt < 1:297807/71‑1 (MQ=255) gCTGGAGCCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGa < 1:1498940/71‑1 (MQ=255) aacctggaacGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTg < 1:2353348/71‑1 (MQ=255) | AACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTG > W3110S.gb/1036339‑1036476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |