| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,084,947 | A→G | 16.9% | K384E (AAA→GAA) | ycdB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,084,947 | 0 | A | G | 16.9% | 139.6 / 21.7 | 59 | K384E (AAA→GAA) | ycdB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/29); new base G (6/4); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCC > W3110S.gb/1084879‑1085017 | aaCTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaa < 1:2429300/71‑1 (MQ=255) ggATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGc > 1:708025/1‑71 (MQ=255) gATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGc < 1:969523/70‑1 (MQ=255) atatGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc < 1:1180173/71‑1 (MQ=255) ggTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGc < 1:498264/71‑1 (MQ=255) gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa < 1:2505808/66‑1 (MQ=255) gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCaa < 1:2646418/66‑1 (MQ=255) gTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCg < 1:1819309/71‑1 (MQ=255) tgatGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATg > 1:4035577/4‑66 (MQ=255) tGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCg > 1:2827408/1‑69 (MQ=255) tGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCg > 1:2090131/1‑69 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:1774455/1‑56 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:1779562/1‑56 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:2886831/1‑56 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGaa < 1:762084/70‑1 (MQ=255) gCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCT‑AATGGc > 1:1210350/1‑66 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCt > 1:1789121/1‑71 (MQ=255) tttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCt > 1:2897463/1‑71 (MQ=255) ttGTCTGCTACCGACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGaa > 1:1486470/1‑65 (MQ=255) ttGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGaa > 1:1803510/1‑65 (MQ=255) tGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTg < 1:1555741/70‑1 (MQ=255) tGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGc < 1:1971133/57‑1 (MQ=255) cTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAgc > 1:3457980/1‑60 (MQ=255) cTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTttagga < 1:4207611/69‑1 (MQ=255) cTACCAACACGATCTGGAAAAAGGCGTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAAt < 1:1541518/71‑1 (MQ=255) tACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCt < 1:1042637/47‑1 (MQ=255) aCCAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaa > 1:446545/1‑40 (MQ=255) aCCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGaa > 1:4165415/1‑68 (MQ=255) ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:640258/1‑41 (MQ=255) ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACg > 1:1335644/1‑71 (MQ=255) ccAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACg > 1:202982/1‑71 (MQ=255) cAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGt > 1:190739/1‑71 (MQ=255) cAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTc < 1:2273124/45‑1 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa < 1:294994/37‑1 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAGGAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTa > 1:4231811/1‑70 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGt < 1:3674586/68‑1 (MQ=255) cacGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaa < 1:2724572/71‑1 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACaaaaaa > 1:1105783/1‑36 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa < 1:2011136/71‑1 (MQ=255) acGATCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTaaa < 1:1190643/71‑1 (MQ=255) cGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAc > 1:3407872/1‑71 (MQ=255) gATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAcc > 1:3332862/1‑71 (MQ=255) aTCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGa < 1:1804393/46‑1 (MQ=255) tCTGGAAAAAGGCTTCCTGACAGTACAAGAAAGGCTCAATGGCGAAg > 1:2325717/1‑47 (MQ=255) cTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGaa > 1:156993/1‑45 (MQ=255) cTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTAt > 1:2230243/1‑71 (MQ=255) tGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCt < 1:934509/49‑1 (MQ=255) tGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTgg < 1:824035/51‑1 (MQ=255) tGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAAt < 1:3317073/57‑1 (MQ=255) ggAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATa > 1:1388329/1‑57 (MQ=255) gAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAAcc < 1:1060417/65‑1 (MQ=255) gAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCt < 1:1169359/66‑1 (MQ=255) aaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTggagga > 1:1449481/1‑51 (MQ=255) aaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTggagg > 1:3159351/1‑50 (MQ=255) aaaaGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATa > 1:861465/1‑54 (MQ=255) gCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCt < 1:3275570/59‑1 (MQ=255) ccTGACAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAtt > 1:313517/1‑71 (MQ=255) ccTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAATCTATCGGCGGCGGtt < 1:1822954/68‑1 (MQ=255) ccTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAt < 1:2860992/70‑1 (MQ=255) gACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGt < 1:1681563/45‑1 (MQ=255) aCAGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt < 1:235429/70‑1 (MQ=255) aCAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt < 1:1223905/70‑1 (MQ=255) aGTACAAGAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTa > 1:761174/1‑63 (MQ=255) gTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTg < 1:3247018/70‑1 (MQ=255) tACAAAAAAGGCTCAATGGCGAAGCGCTTGAGGAATACGTTAAACCTATcg < 1:1974200/51‑1 (MQ=255) aCAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTAttttt < 1:3350851/65‑1 (MQ=255) cAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATa < 1:3951432/35‑1 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTa > 1:345151/1‑43 (MQ=255) aaaaGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGcc > 1:2432841/1‑71 (MQ=255) | AACTGGATATGGGGTTGCTGTTTGTCTGCTACCAACACGATCTGGAAAAAGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCC > W3110S.gb/1084879‑1085017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |