| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,115,786 | T→C | 8.1% | intergenic (‑28/+55) | msyB ← / ← mdtG | hypothetical protein/predicted drug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,115,786 | 0 | T | C | 8.1% | 193.5 / 9.5 | 74 | intergenic (‑28/+55) | msyB/mdtG | hypothetical protein/predicted drug efflux system |
| Reads supporting (aligned to +/- strand): ref base T (35/33); new base C (4/2); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01 | |||||||||||
AATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGG > W3110S.gb/1115726‑1115856 | aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCa > 1:1166849/1‑65 (MQ=255)aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAg > 1:3388935/1‑66 (MQ=255)aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaa > 1:1329271/1‑70 (MQ=255)aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTATCCCTTTGAGTTGATGAGGTTTCAg > 1:3707012/1‑66 (MQ=255) aTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaa > 1:2451634/1‑69 (MQ=255) tGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGttt < 1:3382977/61‑1 (MQ=255) tGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaa > 1:4178932/1‑68 (MQ=255) gCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAgg < 1:3693838/70‑1 (MQ=255) gCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAgg < 1:1062715/70‑1 (MQ=255) cttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGc > 1:350543/1‑71 (MQ=255) cttcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGc > 1:3812036/1‑71 (MQ=255) tcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCt > 1:1080150/1‑70 (MQ=255) tcAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCt < 1:2794045/70‑1 (MQ=255) cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAg < 1:1084126/67‑1 (MQ=255) cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCt < 1:1457313/69‑1 (MQ=255) cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGa > 1:112108/1‑71 (MQ=255) cAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGa > 1:2450150/1‑71 (MQ=255) aaGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGa < 1:3590015/70‑1 (MQ=255) aaGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGAt > 1:4136709/1‑71 (MQ=255) aGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGAtt < 1:4029254/71‑1 (MQ=255) aGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGAtt < 1:2246081/71‑1 (MQ=255) gCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATtc > 1:1001670/1‑71 (MQ=255) cGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaaa > 1:1988680/1‑57 (MQ=255) ttGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTcc > 1:423136/1‑71 (MQ=255) tGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGAt > 1:2138081/1‑58 (MQ=255) tGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGAt > 1:370800/1‑58 (MQ=255) gTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCtt < 1:1299549/71‑1 (MQ=255) tACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTc < 1:3798029/42‑1 (MQ=255) cATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCtttt > 1:2306408/1‑70 (MQ=255) aTGGTCTTCAAGATTGTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGc < 1:2224884/71‑1 (MQ=255) aTGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGa > 1:3087248/1‑57 (MQ=255) tGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCa > 1:927325/1‑39 (MQ=255) tGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaaa > 1:1984384/1‑46 (MQ=255) tGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGc < 1:2364064/70‑1 (MQ=255) catcaCGATTCTCCCTTTGAGCTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAg < 1:2600102/69‑1 (MQ=255) tcaCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGa < 1:936219/50‑1 (MQ=255) tcaCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTAt < 1:1973568/70‑1 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaa > 1:4058177/1‑37 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGaaa > 1:70852/1‑37 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGAt > 1:2089063/1‑42 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:482943/70‑1 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:26166/70‑1 (MQ=255) aCGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGaaa > 1:1426242/1‑37 (MQ=255) cGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGaaa < 1:770906/71‑1 (MQ=255) cGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa > 1:1858795/1‑69 (MQ=255) cGATTCTCCCTTTGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATtctc > 1:2602307/1‑52 (MQ=255) aTTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGaaa < 1:2210689/69‑1 (MQ=255) aTTCTCCCTTTGAGTTGATGAGGCTTCAGGGaaaat < 1:2790263/36‑2 (MQ=39) aTTCTCCCTTTGAGTTGATGAGGCTTCAGGGaaaat < 1:1646426/36‑2 (MQ=39) ctcCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCg > 1:3866733/1‑70 (MQ=255) ccTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGaaaa < 1:3882803/71‑1 (MQ=255) ccTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGaaaa < 1:532614/71‑1 (MQ=255) cTTTGAGTTGATGAGGTTTCAGTGAAAAGGATAGCTGATTCTCCGCtttt < 1:100536/50‑1 (MQ=255) cTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGaaaaa > 1:125107/1‑71 (MQ=255) ttGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCtttt > 1:2864166/1‑48 (MQ=255) ttGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGaa > 1:2481366/1‑59 (MQ=255) ttGAGTTGATGAGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAAt > 1:3663797/1‑70 (MQ=255) tGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCtt > 1:934611/1‑45 (MQ=255) tGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGaaaa < 1:2529402/67‑1 (MQ=255) tGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAAt > 1:217468/1‑69 (MQ=255) tGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATc < 1:331458/70‑1 (MQ=255) tGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATc < 1:1486322/70‑1 (MQ=255) gTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGa < 1:2789319/54‑1 (MQ=255) gatgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGaaaaa > 1:4157614/1‑62 (MQ=255) gatgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCg < 1:891014/70‑1 (MQ=255) gatgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGa > 1:1452430/1‑71 (MQ=255) gatgaGGCTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGa > 1:2137312/1‑71 (MQ=255) atgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGAt < 1:1304201/71‑1 (MQ=255) tgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGAt < 1:358209/70‑1 (MQ=255) tgaGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATa > 1:2578768/1‑71 (MQ=255) aGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGaaa > 1:3599950/1‑49 (MQ=255) gTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGc < 1:2633436/37‑1 (MQ=255) gTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGc < 1:3531031/37‑1 (MQ=255) gTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTg < 1:3241166/71‑1 (MQ=255) gTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTg < 1:3256532/71‑1 (MQ=255) tttCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTgg < 1:3687297/71‑1 (MQ=255) | AATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGG > W3110S.gb/1115726‑1115856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |