| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,224,919 | G→A | 9.4% | intergenic (‑423/‑123) | ymgG ← / → ymgH | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,224,919 | 0 | G | A | 9.4% | 196.7 / 8.3 | 64 | intergenic (‑423/‑123) | ymgG/ymgH | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (26/32); new base A (2/4); total (28/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGATATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAT > W3110S.gb/1224849‑1224988 | aagaTATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCg < 1:4193253/71‑1 (MQ=255) ttCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGt < 1:1195126/66‑1 (MQ=255) atcaGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAg < 1:2715590/70‑1 (MQ=255) caGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtat > 1:3326857/1‑71 (MQ=255) cAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtattat < 1:2889325/71‑1 (MQ=255) aaCGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGa > 1:636498/1‑61 (MQ=255) cGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtattat > 1:1867182/1‑68 (MQ=255) gATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAg > 1:1950491/1‑70 (MQ=255) gATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGa < 1:3875083/71‑1 (MQ=255) gATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGtatt < 1:2407246/65‑1 (MQ=255) aTTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:3020634/71‑1 (MQ=255) aTTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:2266099/71‑1 (MQ=255) aTTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:1514348/71‑1 (MQ=255) ttAAATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaa < 1:1389863/70‑1 (MQ=255) tAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGt > 1:2622092/1‑70 (MQ=255) gTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGa < 1:1819186/70‑1 (MQ=255) attttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttt > 1:228672/1‑71 (MQ=255) ttttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGaaaa < 1:3350491/60‑1 (MQ=255) ttttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttttt < 1:1361883/71‑1 (MQ=255) tttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGtatt > 1:3488671/1‑50 (MQ=255) tttattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGAttttt > 1:3939445/1‑70 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGa < 1:1910260/42‑1 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAg < 1:2610677/54‑1 (MQ=255) ttattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:3958890/1‑71 (MQ=255) tattttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTAt > 1:369820/1‑71 (MQ=255) tattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGa < 1:1389929/41‑1 (MQ=255) tattttGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTa > 1:1184805/1‑70 (MQ=255) tattttGCCGACGGCCTCATTGTCGAAAGATAAGAGTACGACAGTATTATCAGAAAAGAGTGATTTTTTa < 1:504135/70‑1 (MQ=255) ttttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTAt < 1:1970971/69‑1 (MQ=255) ttttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATcc < 1:351213/71‑1 (MQ=255) ttGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:2753859/1‑71 (MQ=255) tGCCGACGGCCTCATTGTCGAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa < 1:3065364/70‑1 (MQ=255) gCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCaa > 1:2747785/1‑69 (MQ=255) gCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAAc < 1:1494131/70‑1 (MQ=255) ccGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAttt > 1:858048/1‑59 (MQ=255) ccGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACt < 1:409483/70‑1 (MQ=255) ccGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACt < 1:1288317/70‑1 (MQ=255) gACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGa < 1:4019215/45‑1 (MQ=255) aCGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTacac > 1:1354689/1‑71 (MQ=255) cTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAg > 1:683197/1‑71 (MQ=255) tCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgc > 1:4122432/1‑71 (MQ=255) cATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttttt < 1:1433490/52‑1 (MQ=255) cATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgcg > 1:3872060/1‑71 (MQ=255) aTTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgc < 1:1304911/69‑1 (MQ=255) ttGTCGAAAGATAGGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCa < 1:1567011/71‑1 (MQ=255) ttGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAgcg < 1:4239281/69‑1 (MQ=255) ttGTAGAAAGATAAGCGTACGACAGTATTATCAGAAAaga > 1:3973402/1‑40 (MQ=255) tGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTAt > 1:3371047/1‑51 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGaaaa < 1:2735038/36‑1 (MQ=255) gTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTAt > 1:454554/1‑50 (MQ=255) gTCGAAAGATAAGCGTACGACAGTAATATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt > 1:3548524/1‑71 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttt > 1:447054/1‑45 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGAtttt > 1:442031/1‑45 (MQ=255) tCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACt < 1:2625372/70‑1 (MQ=255) gAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCg > 1:1549496/1‑71 (MQ=255) gAAAGATAAGCATACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGc < 1:3139405/70‑1 (MQ=255) aaaGATAAGCGTACGACAGTATTATCAGAAAagag < 1:873254/35‑1 (MQ=255) aaaGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTa > 1:928424/1‑53 (MQ=255) aaaGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGc < 1:1086395/69‑1 (MQ=255) aaaGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCgt > 1:42584/1‑71 (MQ=255) aGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCgtg > 1:1611207/1‑70 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaaa < 1:2637257/71‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:2322968/70‑1 (MQ=255) aGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaaaa < 1:2693601/70‑1 (MQ=255) gCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTaa < 1:2543319/67‑1 (MQ=255) gTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCa > 1:1512232/1‑55 (MQ=255) gTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCa > 1:1232463/1‑55 (MQ=255) gTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAt < 1:1186153/70‑1 (MQ=255) | AAGATATTCATCAGCAACGATTACATTAGTCATTTTATTTTGCCGACGGCCTCATTGTCGAAAGATAAGCGTACGACAGTATTATCAGAAAAGAGTGATTTTTTATCCAACTACACTTCAGCGCACTGCGTGTAAAAAAT > W3110S.gb/1224849‑1224988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |