| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 45.5% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 45.5% | 24.4 / 113.4 | 66 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (17/19); new base G (16/14); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01 | |||||||||||
GGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGG > W3110S.gb/70670‑70809 | gggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt > 1:119225/1‑71 (MQ=255)gggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt > 1:3226390/1‑71 (MQ=255)gggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt > 1:1745323/1‑71 (MQ=255) ttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:2788082/1‑71 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctt < 1:3482731/70‑1 (MQ=255) cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt > 1:1779433/1‑70 (MQ=255) tttCGTCTGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttcttt < 1:593781/69‑1 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCg > 1:2236308/1‑71 (MQ=255) tttCGTCCGCGCGCCTAATGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCg > 1:1072241/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:3012275/1‑71 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGccc > 1:3530622/1‑71 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttcttt > 1:4204304/1‑62 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGAt > 1:1901308/1‑70 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg < 1:2059949/71‑1 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGa < 1:3988825/71‑1 (MQ=255) cgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg > 1:390848/1‑70 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgc > 1:346048/1‑71 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgc > 1:2496058/1‑71 (MQ=255) aCTGGCTTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:701890/47‑1 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc > 1:1467249/1‑71 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCatg > 1:3928901/1‑69 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:187879/1‑70 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCa < 1:2392629/69‑1 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAg < 1:3757459/70‑1 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCa < 1:2103010/69‑1 (MQ=255) aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGAt < 1:3651052/56‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgtt < 1:502721/42‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg > 1:2473955/1‑56 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt < 1:2401602/71‑1 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGccc > 1:3039305/1‑49 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa < 1:535841/70‑1 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:1418438/1‑51 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc > 1:3759149/1‑65 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:225661/71‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCgg > 1:2052967/1‑47 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc > 1:463140/1‑70 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc < 1:783034/70‑1 (MQ=255) cTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:821742/37‑1 (MQ=255) tAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:961693/1‑70 (MQ=255) tAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:1447367/1‑70 (MQ=255) tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc > 1:614291/1‑70 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc < 1:1480086/69‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc < 1:3479205/69‑1 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAcc < 1:3739515/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCTCATTTCAGCGACCTGt < 1:2313958/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg < 1:3190033/70‑1 (MQ=255) gCCGTCAATATATGCCAATACGGGTTTCTTTCGCCCg < 1:1645832/37‑1 (MQ=255) ccGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:897988/71‑1 (MQ=255) ccGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:3630661/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:4262216/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:1655854/71‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg < 1:2835223/71‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg > 1:4181616/1‑71 (MQ=255) aaTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:3569686/1‑70 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:2671152/64‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:572956/71‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa > 1:3809367/1‑70 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcgc > 1:1393402/1‑39 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:3456767/71‑1 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa > 1:2130817/1‑70 (MQ=255) atTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:1954066/70‑1 (MQ=255) atTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCa < 1:2255207/42‑1 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt > 1:2142183/1‑62 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:3911935/71‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc > 1:4120508/1‑57 (MQ=255) tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGa > 1:1790794/1‑54 (MQ=255) gCCAATACGGGGATCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa < 1:1282617/70‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:1719143/71‑1 (MQ=255) cAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt > 1:3229019/1‑70 (MQ=255) cAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt > 1:3195731/1‑70 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt < 1:1580987/44‑1 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt > 1:1174668/1‑70 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:1135074/43‑1 (MQ=255) tACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:3933767/54‑1 (MQ=255) aCGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc > 1:2186276/1‑48 (MQ=255) aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg < 1:3195126/51‑1 (MQ=255) gTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCggg > 1:3535062/1‑71 (MQ=255) | GGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGG > W3110S.gb/70670‑70809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |