| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,516,711 | C→T | 17.2% | A79V (GCG→GTG) | ydcV → | predicted spermidine/putrescine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,516,711 | 0 | C | T | 17.2% | 69.3 / 7.8 | 29 | A79V (GCG→GTG) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (2/3); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTC > W3110S.gb/1516641‑1516780 | gcaCAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTgc < 1:1906252/71‑1 (MQ=255) acaGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTgtgc > 1:419479/1‑71 (MQ=255) caGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCt < 1:1071891/71‑1 (MQ=255) gTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGtt < 1:430438/70‑1 (MQ=255) gTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGtt < 1:543415/70‑1 (MQ=255) gTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTa < 1:1629080/71‑1 (MQ=255) atatTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAgg < 1:1453457/70‑1 (MQ=255) atatTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAgg < 1:820439/70‑1 (MQ=255) cTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCt > 1:1811435/1‑71 (MQ=255) ttGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAGGGACGCTg > 1:699098/1‑71 (MQ=255) ttGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGa > 1:2582190/1‑66 (MQ=255) tGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAg < 1:3009589/71‑1 (MQ=255) gTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGTGCTGGTGTTAg > 1:205200/1‑55 (MQ=255) acacTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCcgc > 1:2203147/1‑71 (MQ=255) gTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTgg < 1:3643867/41‑1 (MQ=255) tCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCtgtg > 1:4247292/1‑71 (MQ=255) tCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCtgt < 1:3050661/70‑1 (MQ=255) tCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCtg < 1:1405450/69‑1 (MQ=255) cACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGtt > 1:2951884/1‑43 (MQ=255) cACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGtt > 1:3904905/1‑43 (MQ=255) cTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAgg < 1:634340/44‑1 (MQ=255) ttAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcga > 1:2216273/1‑71 (MQ=255) aaaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGcgac > 1:2291734/1‑70 (MQ=255) cggcgCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACttt < 1:2062134/71‑1 (MQ=255) gcgcTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACttttt < 1:911651/71‑1 (MQ=255) tGGCGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt < 1:1846531/49‑1 (MQ=255) gCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCa > 1:345967/1‑70 (MQ=255) cGACATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaaa < 1:3957644/71‑1 (MQ=255) gACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGCCaaaa < 1:3587695/71‑1 (MQ=255) cATTAATTGTGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCt < 1:785822/43‑1 (MQ=255) aaTTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCaaaaa > 1:3878335/1‑66 (MQ=255) gcgcTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCAttt < 1:3186863/70‑1 (MQ=255) gcgcTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTc > 1:3978408/1‑71 (MQ=255) | GCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTC > W3110S.gb/1516641‑1516780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |