| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,819,760 | G→T | 10.8% | A152E (GCG→GAG) | chbF ← | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,819,760 | 0 | G | T | 10.8% | 141.7 / 3.3 | 46 | A152E (GCG→GAG) | chbF | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (18/23); new base T (3/2); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
ATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAA > W3110S.gb/1819694‑1819828 | atatTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCccgccg < 1:1708854/70‑1 (MQ=255) ttACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCTCCGGGt > 1:1580718/1‑70 (MQ=255) acacGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAg < 1:3074812/69‑1 (MQ=255) cacGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGt < 1:3560858/65‑1 (MQ=255) acGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTg < 1:3081208/69‑1 (MQ=255) cGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAg < 1:404898/71‑1 (MQ=255) gCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGaa > 1:2184185/1‑69 (MQ=255) cGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGtt < 1:1270712/70‑1 (MQ=255) cGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTa < 1:1527132/71‑1 (MQ=255) aTAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGtt < 1:853448/68‑1 (MQ=255) aaaGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCa > 1:542433/1‑71 (MQ=255) aaaGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCa > 1:4179110/1‑71 (MQ=255) aaaGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCa > 1:430401/1‑71 (MQ=255) aaaGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCa > 1:2046443/1‑71 (MQ=255) aaaGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCa > 1:1930856/1‑71 (MQ=255) aGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGaa > 1:2560412/1‑61 (MQ=255) aGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCAcc < 1:1840144/71‑1 (MQ=255) tttAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTaa < 1:3851877/62‑1 (MQ=255) tttAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATg < 1:2058416/71‑1 (MQ=255) tAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTaa > 1:3794481/1‑60 (MQ=255) aaTCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCAt < 1:3339381/70‑1 (MQ=255) cGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGa > 1:2610043/1‑71 (MQ=255) ggTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTGGt < 1:1154692/45‑1 (MQ=255) tGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGt < 1:3361706/37‑1 (MQ=255) gACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGa < 1:143810/42‑1 (MQ=255) aCGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGtt < 1:3179348/71‑1 (MQ=255) aTAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTc > 1:1594313/1‑58 (MQ=255) tAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCg < 1:106714/58‑1 (MQ=255) tAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGttcttc < 1:15972/71‑1 (MQ=255) aaaCGGCTTCAGTGACCATTCCCGCCGGGTTAGTGa > 1:930384/1‑36 (MQ=255) aaaCGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATg > 1:588991/1‑51 (MQ=255) aaCGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACa > 1:2106124/1‑60 (MQ=255) aCGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCATTCgg < 1:3326927/56‑1 (MQ=255) aCGGCTTCAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCATTCgg < 1:821137/56‑1 (MQ=255) ggCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACAt < 1:32236/71‑1 (MQ=255) ggCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACAt < 1:3608436/71‑1 (MQ=255) cAGTGACCATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCttt > 1:3285882/1‑70 (MQ=255) cAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCttt > 1:1239854/1‑70 (MQ=255) cAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCttt > 1:1557950/1‑70 (MQ=255) cAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCttt > 1:1660835/1‑70 (MQ=255) cAGTGAACATTCCCTCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCtt > 1:856016/1‑69 (MQ=255) gTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTAc < 1:2474106/71‑1 (MQ=255) gTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTAc < 1:253821/71‑1 (MQ=255) tGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGttc < 1:2483527/55‑1 (MQ=255) cATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATg < 1:2499469/70‑1 (MQ=255) cccGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCtttt > 1:895307/1‑60 (MQ=255) cccGCCGGGTTAGTGAAGTTAATCACCCATGCATTAGGACAAAGTTCTTCGACATCTTTTa > 1:850885/1‑61 (MQ=255) cacgccgGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGttct > 1:2610141/3‑48 (MQ=255) ccgccgGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCaaaa < 1:3022205/71‑1 (MQ=255) | ATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAA > W3110S.gb/1819694‑1819828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |