| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,872,347 | A→G | 7.7% | K83K (AAA→AAG) | yeaI → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,872,347 | 0 | A | G | 7.7% | 134.9 / 4.4 | 52 | K83K (AAA→AAG) | yeaI | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (29/19); new base G (2/2); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
TCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCT > W3110S.gb/1872280‑1872415 | tCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAGCt < 1:3483190/70‑1 (MQ=255) cAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTc < 1:2227938/70‑1 (MQ=255) tATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCca > 1:2711647/1‑71 (MQ=255) aTTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTaaaa > 1:2650195/1‑61 (MQ=255) gCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACt > 1:2976029/1‑60 (MQ=255) cTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGa < 1:1225227/70‑1 (MQ=255) tGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAGCTCATCCCACAGAt > 1:4021087/1‑70 (MQ=255) gACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATg > 1:3376441/1‑70 (MQ=255) gACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATg > 1:211037/1‑70 (MQ=255) aCGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGaa > 1:1960336/1‑71 (MQ=255) aCGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGaa > 1:1840993/1‑71 (MQ=255) gTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAAtt < 1:2356288/71‑1 (MQ=255) gTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAAt > 1:1173044/1‑70 (MQ=255) aaTTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTaaaa > 1:3721355/1‑49 (MQ=255) ttattttGCATTACTACCTTATATAACGAAAACACAAATGTaaaa > 1:3182321/1‑45 (MQ=255) ttattttGCATTACTACCTTATATAACGAAAACACAAATGTaaaa > 1:1765983/1‑45 (MQ=255) tttGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCAt < 1:4037840/46‑1 (MQ=255) tGCATTACTACCTTATATAACGAAAACACAAATGTaaaa < 1:1075902/39‑1 (MQ=255) tGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGgttgt > 1:913767/1‑71 (MQ=255) tGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGgttgt > 1:1392705/1‑71 (MQ=255) gCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGgttgt < 1:1307904/70‑1 (MQ=255) aTTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTtgttgt < 1:3553710/71‑1 (MQ=255) ttACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATg < 1:4272609/50‑1 (MQ=255) ttACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGa < 1:1788818/51‑1 (MQ=255) actacCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAAt > 1:4176323/1‑51 (MQ=255) ctacCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTa > 1:365861/1‑52 (MQ=255) ctacCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATCAATTAcc > 1:2630371/1‑54 (MQ=255) acCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAAtt < 1:214167/49‑1 (MQ=255) ccTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCttt > 1:3834559/1‑70 (MQ=255) ccTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCtt < 1:1446915/69‑1 (MQ=255) cTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGt < 1:3463320/71‑1 (MQ=255) cTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGt > 1:1847393/1‑71 (MQ=255) ttATATAACGAAAACACAAATGTAAAACTCATCCcac < 1:605896/37‑1 (MQ=255) ttATATAACGAAAACACAAATGTAAAACTCATCCCACAGATg < 1:3557266/42‑1 (MQ=255) tatataACGAAAACACAAATGTAAAACTCATCCCACAGa > 1:215322/1‑39 (MQ=255) atataACGAAAACACAAATGTAAAACTCATCCcac > 1:1862949/1‑35 (MQ=255) atataACGAAAACACAAATGTAAAACTCATCCCACAg > 1:2631508/1‑37 (MQ=255) atataACGAAAACACAAATGTAAAACTCATCCCACAg > 1:2081449/1‑37 (MQ=255) atataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGtttt > 1:3151463/1‑71 (MQ=255) atataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGtttt > 1:3216790/1‑71 (MQ=255) tataACGAAAACACAAATGTAAAGCTCATCCCACAGa < 1:1362668/37‑1 (MQ=255) tataACGAAAACACAAATGTAAAACTCATCCCACAGATGAAt > 1:627316/1‑42 (MQ=255) tataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGttttt > 1:3444702/1‑71 (MQ=255) ataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:3127374/1‑71 (MQ=255) ataACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc > 1:2336305/1‑71 (MQ=255) taACGAAAACACAAATGTAAAACTCATCCCACAGATGa < 1:2680697/38‑1 (MQ=255) aaCGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCt > 1:1326364/1‑71 (MQ=255) gAAAACACAAATGTAAAGCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTaa > 1:531785/1‑71 (MQ=255) gAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTaa > 1:3046632/1‑71 (MQ=255) aaaaCACATATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTc < 1:171181/65‑1 (MQ=255) aaaaCACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGgt < 1:2063157/46‑1 (MQ=255) aaaCACAAATGTAAAACTCATCCCACAGATGAATTa > 1:1086164/1‑36 (MQ=255) aaCACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCt > 1:4238936/1‑54 (MQ=255) aaCACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGc < 1:3505670/71‑1 (MQ=255) aaCACAAATGTAAAACTCATCACACAGATGAATTACCTGATGgttgt < 1:2551265/47‑1 (MQ=255) acacAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTtgttgt > 1:2831845/1‑49 (MQ=255) tAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCg < 1:1377697/63‑1 (MQ=255) aaaCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATttttct < 1:1536871/71‑1 (MQ=255) aaaCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATttttct < 1:3922566/71‑1 (MQ=255) aaaCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATttttct < 1:1784619/71‑1 (MQ=255) aCTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGc > 1:1182157/1‑57 (MQ=255) | TCAGTTTATTGCTGACGTTAATTTTATTTTGCATTACTACCTTATATAACGAAAACACAAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCT > W3110S.gb/1872280‑1872415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |