| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,989,353 | T→A | 6.3% | S98T (TCT→ACT) | yecI → | predicted ferritin‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,989,353 | 0 | T | A | 6.3% | 171.4 / 4.0 | 63 | S98T (TCT→ACT) | yecI | predicted ferritin‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (32/27); new base A (2/2); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
GTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCT > W3110S.gb/1989284‑1989420 | gTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTc > 1:2152622/1‑71 (MQ=255) ttCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTc < 1:1676218/70‑1 (MQ=255) cGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTa > 1:4070895/1‑71 (MQ=255) gTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACg > 1:3106574/1‑71 (MQ=255) gTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACg > 1:3101792/1‑71 (MQ=255) aaaaCTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTgg > 1:1207154/1‑71 (MQ=255) aCTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTgg > 1:1005486/1‑68 (MQ=255) aCTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGcac < 1:4037170/71‑1 (MQ=255) cTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGca > 1:1724183/1‑69 (MQ=255) ctctGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGcc > 1:1411295/1‑71 (MQ=255) tGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGAt > 1:1484627/1‑71 (MQ=255) ggAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGcac < 1:2299111/59‑1 (MQ=255) gaagaaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATg < 1:2397629/70‑1 (MQ=255) aagaaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATg > 1:3668621/1‑69 (MQ=255) aCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGa < 1:975676/71‑1 (MQ=255) cTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGaa < 1:3583339/71‑1 (MQ=255) tCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGaa > 1:2712975/1‑71 (MQ=255) ccAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACg > 1:3045227/1‑40 (MQ=255) ccAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGcaca > 1:2591661/1‑48 (MQ=255) ccAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGaa < 1:481235/70‑1 (MQ=255) ccAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAAc > 1:1975969/1‑71 (MQ=255) aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGa < 1:1924712/58‑1 (MQ=255) aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACt > 1:2311721/1‑70 (MQ=255) aaaaaCGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGcac < 1:602557/44‑1 (MQ=255) aaaCGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAat < 1:287349/71‑1 (MQ=255) aaCGATGGAAGAATACGAGCAACGTTCTAGTAGGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAat < 1:564947/70‑1 (MQ=255) aaCGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatg < 1:2378276/71‑1 (MQ=255) cGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatgat < 1:788572/71‑1 (MQ=255) gATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatgatg < 1:3424932/71‑1 (MQ=255) gATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatgatg < 1:1526557/71‑1 (MQ=255) tGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACt > 1:67219/1‑61 (MQ=255) tGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACt > 1:2580830/1‑61 (MQ=255) tGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACt > 1:2575722/1‑61 (MQ=255) aagaaTACGATCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGaa > 1:2597295/1‑56 (MQ=255) agaaTACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatgatg > 1:1640498/1‑65 (MQ=255) aaTACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAAcc < 1:2824126/71‑1 (MQ=255) aTACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAAcc < 1:841721/70‑1 (MQ=255) aTACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCg < 1:1975789/71‑1 (MQ=255) tACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAg > 1:2863778/1‑43 (MQ=255) tACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGt > 1:638149/1‑71 (MQ=255) aCGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCg > 1:2374921/1‑36 (MQ=255) aCGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGt > 1:1190295/1‑70 (MQ=255) cGAGCAACGTTCTAGTACGTTGTCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTc > 1:582570/1‑70 (MQ=255) cGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAg > 1:3425003/1‑47 (MQ=255) cGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAg > 1:1128228/1‑47 (MQ=255) cGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAAc > 1:3879954/1‑66 (MQ=255) cGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTc > 1:2209999/1‑70 (MQ=255) cGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCa < 1:1887058/71‑1 (MQ=255) gCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAtt < 1:341793/71‑1 (MQ=255) ccAACGTACTAGAACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGt < 1:1604204/65‑1 (MQ=39) ccAACGTACTAGAACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGt < 1:3826346/65‑1 (MQ=39) cAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAtgatga > 1:100485/1‑56 (MQ=255) aaCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGa < 1:1008349/39‑1 (MQ=255) aaCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTg < 1:3066032/47‑1 (MQ=255) aaCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAttt < 1:4075592/70‑1 (MQ=255) aaCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTc < 1:806378/71‑1 (MQ=255) aaCGTACTAGAACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCa > 1:3293269/1‑66 (MQ=255) aaCGTACTAGAACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCa > 1:1578734/1‑66 (MQ=255) aCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACt > 1:3815623/1‑45 (MQ=255) aCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatgatg < 1:2504854/53‑1 (MQ=255) aCGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTc < 1:451451/70‑1 (MQ=255) cGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCt > 1:3366901/1‑71 (MQ=255) gTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTc < 1:1996702/62‑1 (MQ=255) ttCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCaa > 1:317425/1‑63 (MQ=255) | GTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCT > W3110S.gb/1989284‑1989420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |