| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,170,757 | T→C | 8.0% | intergenic (‑314/‑92) | yegR ← / → yegS | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,170,757 | 0 | T | C | 8.0% | 203.5 / 9.3 | 75 | intergenic (‑314/‑92) | yegR/yegS | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (38/31); new base C (3/3); total (41/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
CCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTA > W3110S.gb/2170687‑2170816 | ccGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACt > 1:814704/1‑71 (MQ=255)ccGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACt > 1:3151827/1‑71 (MQ=255)ccGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACt > 1:2944239/1‑71 (MQ=255) gTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACttt < 1:3264672/71‑1 (MQ=255) ttAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACtttt > 1:242424/1‑71 (MQ=255) ttAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACtttt > 1:3179435/1‑71 (MQ=255) aGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTa > 1:724767/1‑71 (MQ=255) ttCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcct > 1:1146053/1‑71 (MQ=255) atCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcc < 1:2574879/69‑1 (MQ=255) cGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcctcc > 1:421135/1‑71 (MQ=255) cGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACCTTTTAtcctcc > 1:767639/1‑71 (MQ=255) gCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACttttt < 1:3016075/63‑1 (MQ=255) gCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcctcc < 1:3578603/70‑1 (MQ=255) gCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCg < 1:341805/71‑1 (MQ=255) cccATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTc < 1:1049307/71‑1 (MQ=255) aTTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATc > 1:2919354/1‑71 (MQ=255) tGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACttt > 1:2671792/1‑50 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcctcc > 1:1948895/1‑58 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAtcctcc > 1:1632186/1‑58 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCAt > 1:2327362/1‑67 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg > 1:1900429/1‑71 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg > 1:1585198/1‑71 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg > 1:962738/1‑71 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg > 1:2668921/1‑71 (MQ=255) gCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTCTTTATCCTCCGACTTTCATCCt < 1:1608015/70‑1 (MQ=255) cAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg < 1:185039/70‑1 (MQ=255) cAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg < 1:4194162/70‑1 (MQ=255) aaGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGtt > 1:2356108/1‑71 (MQ=255) aaGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGtt < 1:1096924/71‑1 (MQ=255) gCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:3201957/71‑1 (MQ=255) gCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:1627188/71‑1 (MQ=255) gCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCt < 1:14026/71‑1 (MQ=255) cTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTg < 1:1087226/71‑1 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTAt > 1:1888296/1‑45 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGt < 1:346773/64‑1 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGAc < 1:2218938/70‑1 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACt > 1:3905069/1‑71 (MQ=255) cTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACt > 1:599336/1‑71 (MQ=255) ggtggtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:598452/1‑67 (MQ=255) gtggtAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCa < 1:652637/71‑1 (MQ=255) gtATCCACTGAATCCTCCATACTTGAACTGACttt < 1:1937238/35‑1 (MQ=255) gtAACCACTGAATCCTCCATACTTTAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCt < 1:3146465/61‑1 (MQ=255) gtAACCACTGAATCCTCCATACTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCt < 1:2282345/61‑1 (MQ=255) tAACCACTGAATCCTCCATACTTGAACTGACtttt < 1:4134010/35‑1 (MQ=255) tAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCtt < 1:4200383/71‑1 (MQ=255) aCCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCtttt < 1:1329703/71‑1 (MQ=255) ccACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:724005/57‑1 (MQ=255) ccACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:2274804/57‑1 (MQ=255) aCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:748225/71‑1 (MQ=255) aCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:940683/71‑1 (MQ=255) aCTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:2856534/71‑1 (MQ=255) gAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTCTTCTGACTcc > 1:907300/1‑58 (MQ=255) aaTCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCAt > 1:733115/1‑43 (MQ=255) aaTCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTc < 1:3416069/70‑1 (MQ=255) aaTCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTc < 1:2874250/70‑1 (MQ=255) tcctccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCt > 1:3144921/1‑69 (MQ=255) cctccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:3306368/48‑1 (MQ=255) ctccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtttt > 1:2799192/1‑65 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTg < 1:2236310/41‑1 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGt < 1:3998470/42‑1 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCAc > 1:528058/1‑53 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCAc > 1:972529/1‑53 (MQ=255) ccATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtttt > 1:306857/1‑63 (MQ=255) cATACTTGAACTGAGTTTTTATCATCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa < 1:1530692/70‑1 (MQ=255) cATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa > 1:4037954/1‑70 (MQ=255) cATACTTGAACAGACTTTTTATCCTCCGACTTTCATc > 1:3998893/1‑37 (MQ=255) aTACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCGAACTTTTGTTTTCTGCTCTaca > 1:4192853/1‑71 (MQ=255) aTACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTaca > 1:2727416/1‑71 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:2292203/42‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCt < 1:3631547/42‑1 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCt > 1:2336951/1‑62 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTacac > 1:2907042/1‑71 (MQ=255) tACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTaca > 1:2911883/1‑70 (MQ=255) aCTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGtt < 1:2751411/57‑1 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:3824510/1‑42 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGa > 1:3988920/1‑42 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTaca > 1:4223149/1‑68 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTaca > 1:1262619/1‑68 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTa > 1:541528/1‑66 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTa > 1:2021554/1‑71 (MQ=255) cTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTa > 1:1781186/1‑71 (MQ=255) cTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCttt > 1:3826611/1‑52 (MQ=255) cTTGAACTGACCTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCttt > 1:3941974/1‑52 (MQ=255) tGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGctc < 1:1326429/57‑1 (MQ=255) aCTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTg > 1:4095342/1‑45 (MQ=255) tttttATCCTCCGACTTTCATCCTGTTCTGACTCCa > 1:1590907/1‑36 (MQ=255) | CCGTTAGTATTCGCTGTCCCCATTGCAAGCTCCTGGTGGTAACCACTGAATCCTCCATACTTGAACTGACTTTTTATCCTCCGACTTTCATCCTGTTCTGACTCCACCTTTTGTTTTCTGCTCTACACTA > W3110S.gb/2170687‑2170816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |