| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,001,511 | T→C | 5.5% | I81T (ATA→ACA) | xdhB → | xanthine dehydrogenase, FAD‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,001,511 | 0 | T | C | 5.5% | 204.9 / 3.7 | 73 | I81T (ATA→ACA) | xdhB | xanthine dehydrogenase, FAD‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (37/32); new base C (2/2); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCG > W3110S.gb/3001444‑3001578 | cGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:2503097/71‑1 (MQ=255)cGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:3302695/71‑1 (MQ=255)cGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:2301693/71‑1 (MQ=255)cGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATag > 1:854994/1‑70 (MQ=255) aaTTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATc > 1:1674974/1‑71 (MQ=255) aTTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATc < 1:1593663/70‑1 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:2675833/1‑70 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:31602/1‑70 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:1861427/1‑70 (MQ=255) cGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCtat < 1:814379/70‑1 (MQ=255) tGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAAc > 1:2741611/1‑70 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTc > 1:2492754/1‑70 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTc > 1:3190553/1‑70 (MQ=255) cGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACagaaga < 1:161425/58‑1 (MQ=255) ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAAc > 1:4208531/1‑71 (MQ=255) aGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTc < 1:3647392/71‑1 (MQ=255) gATGGCTCGCTACGTATCGGCTCTGTAACGACATTTACCCAGCTAATAGAAGATc < 1:3277597/55‑1 (MQ=255) gATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCtat < 1:1440684/59‑1 (MQ=255) cTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:3242237/1‑46 (MQ=255) cGCTACGTATCGTCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:1068567/1‑71 (MQ=255) cGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:632923/1‑71 (MQ=255) cGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCg > 1:2081397/1‑71 (MQ=255) gCTACGAATCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:549996/1‑43 (MQ=255) tACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCgg < 1:3280217/69‑1 (MQ=255) aCGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCa > 1:3724681/1‑54 (MQ=255) aCGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt > 1:3617718/1‑71 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaag > 1:4000248/1‑40 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCaa > 1:3249792/1‑54 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCg < 1:1645635/69‑1 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt < 1:2465326/70‑1 (MQ=255) gTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt < 1:383846/69‑1 (MQ=255) gTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa < 1:1879052/71‑1 (MQ=255) gTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa > 1:1374361/1‑71 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:4273824/1‑37 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATc < 1:613870/41‑1 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGc < 1:1594828/66‑1 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAt > 1:4162341/1‑71 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAt > 1:2158667/1‑71 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCt < 1:570799/41‑1 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTcc < 1:2657526/60‑1 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTcc < 1:1821482/60‑1 (MQ=255) gCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt < 1:1703445/64‑1 (MQ=255) gCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgct < 1:364475/71‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTc < 1:3507932/41‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtg < 1:2444765/63‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgctgc < 1:654388/69‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg > 1:2797989/1‑71 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg < 1:2974203/69‑1 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:138538/1‑70 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:2562344/1‑70 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:406193/1‑70 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:1558267/1‑70 (MQ=255) aaCGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAtct < 1:3727756/48‑1 (MQ=255) aCGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCAc < 1:1927038/71‑1 (MQ=255) cGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACg > 1:2393378/1‑71 (MQ=255) cGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACg > 1:255753/1‑71 (MQ=255) aCATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc > 1:865166/1‑71 (MQ=255) aCATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc > 1:2870504/1‑71 (MQ=255) aCATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc > 1:2539163/1‑71 (MQ=255) cATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtgtg > 1:903835/1‑57 (MQ=255) aTTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtgt < 1:3868940/55‑1 (MQ=255) ttACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtg < 1:931782/52‑1 (MQ=255) aCCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCAc > 1:3638100/1‑62 (MQ=255) cccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCt < 1:2462583/71‑1 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCt > 1:3468017/1‑70 (MQ=255) cAGCTAATAGAAGATCCTATTACTCAACGTCATCTCCCgg < 1:946898/40‑1 (MQ=255) aGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGa > 1:1566451/1‑71 (MQ=255) gCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtg < 1:2331900/45‑1 (MQ=255) cTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGt < 1:979024/40‑1 (MQ=255) tAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg > 1:1813819/1‑71 (MQ=255) tAATAGAAGATCATATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg > 1:3431141/1‑71 (MQ=255) aTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg < 1:3074462/69‑1 (MQ=255) aTAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg > 1:247937/1‑69 (MQ=255) tAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgct < 1:609270/44‑1 (MQ=255) tAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGt < 1:3283554/54‑1 (MQ=255) | CGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCG > W3110S.gb/3001444‑3001578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |