| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,139,938 | C→T | 6.4% | R2H (CGT→CAT) | hybD ← | predicted maturation element for hydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,139,938 | 0 | C | T | 6.4% | 253.4 / 5.4 | 78 | R2H (CGT→CAT) | hybD | predicted maturation element for hydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base C (39/34); new base T (3/2); total (42/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGC > W3110S.gb/3139868‑3140004 | cGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATAc < 1:2059020/71‑1 (MQ=255) ttCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa > 1:2433586/1‑71 (MQ=255) cGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt > 1:1375701/1‑70 (MQ=255) cGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt < 1:3211049/70‑1 (MQ=255) cGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt > 1:1519188/1‑70 (MQ=255) aCAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa > 1:2465303/1‑70 (MQ=255) aCAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa > 1:2852940/1‑70 (MQ=255) cAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa > 1:643452/1‑69 (MQ=255) cAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa < 1:2861315/71‑1 (MQ=255) ccGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAc > 1:2212506/1‑71 (MQ=255) ccGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAc > 1:4237391/1‑71 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc < 1:1796118/71‑1 (MQ=255) gCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt < 1:3253079/71‑1 (MQ=255) gCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCt > 1:3599810/1‑71 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt > 1:3486984/1‑71 (MQ=255) acacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt < 1:1745165/70‑1 (MQ=255) acacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt > 1:3458468/1‑70 (MQ=255) acacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc < 1:380189/71‑1 (MQ=255) gATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt > 1:1834535/1‑69 (MQ=255) aTGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGa > 1:334305/1‑70 (MQ=255) gCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAc > 1:346873/1‑70 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAcc > 1:2473586/1‑68 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt > 1:893563/1‑71 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt > 1:1346510/1‑71 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt > 1:4240217/1‑71 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACtt > 1:234819/1‑71 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACtt > 1:678589/1‑71 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACtt > 1:4065556/1‑71 (MQ=255) aTCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt < 1:4143233/69‑1 (MQ=255) aTCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc < 1:3860026/71‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg < 1:3263704/60‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGa < 1:2222414/61‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc < 1:3123489/70‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg < 1:3971269/71‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg < 1:3050577/71‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg < 1:382943/71‑1 (MQ=255) cGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGAATTACAGAACCTTCACTGAAACCACTTCGt > 1:2229295/1‑71 (MQ=255) ggTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGtt < 1:3194515/71‑1 (MQ=255) cAGCAAAATATTGCCGACGCCTAAGACTTAAATACGCTTTACAGAACCTTCACt < 1:1948799/54‑1 (MQ=255) gCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt < 1:2198066/71‑1 (MQ=255) gCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt > 1:2911837/1‑71 (MQ=255) gCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt < 1:1656013/71‑1 (MQ=255) gCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt < 1:2052435/71‑1 (MQ=255) aaaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg > 1:4004382/1‑51 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAg < 1:2666577/71‑1 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACAGaa > 1:1536155/1‑52 (MQ=255) aaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt > 1:860468/1‑48 (MQ=255) atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa > 1:1733665/1‑71 (MQ=255) atatTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg > 1:2853659/1‑48 (MQ=255) atatTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa > 1:3394445/1‑71 (MQ=255) tatTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCAt > 1:2571429/1‑71 (MQ=255) ttGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:2384215/70‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc > 1:710780/1‑70 (MQ=255) ttGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc > 1:599265/1‑70 (MQ=255) ccGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATcc < 1:3947834/68‑1 (MQ=255) ccGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACt < 1:2484737/71‑1 (MQ=255) aCCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACg > 1:1489158/1‑71 (MQ=255) aCCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACg > 1:252603/1‑71 (MQ=255) ccccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgt > 1:759366/1‑71 (MQ=255) ccccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgt < 1:1556375/71‑1 (MQ=255) ccccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgt < 1:3040201/71‑1 (MQ=255) cccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtg < 1:3096759/71‑1 (MQ=255) ccTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGt < 1:3093450/54‑1 (MQ=255) cTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGc < 1:2605800/57‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCa < 1:681808/55‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCa < 1:2853003/58‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCa > 1:2214797/1‑62 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATcc < 1:650315/60‑1 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTTaa > 1:2287083/1‑35 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAcc > 1:1738895/1‑71 (MQ=255) aaGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAcc > 1:2215757/1‑71 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCg < 1:1942545/71‑1 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCg < 1:2318879/71‑1 (MQ=255) aGACTAAAATACGCATTACAGAACCATCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAccc < 1:3327086/71‑2 (MQ=255) aCTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGtt > 1:2523409/1‑43 (MQ=255) tAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCa < 1:3624154/48‑1 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAgg > 1:3859863/1‑71 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTa > 1:3157648/1‑55 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGc < 1:607039/71‑1 (MQ=255) | CGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGC > W3110S.gb/3139868‑3140004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |