| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,161,757 | G→A | 8.5% | T127T (ACC→ACT) | plsC ← | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,161,757 | 0 | G | A | 8.5% | 142.4 / 3.8 | 47 | T127T (ACC→ACT) | plsC | 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base A (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
TGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACT > W3110S.gb/3161687‑3161821 | cgCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATg > 1:1732235/2‑71 (MQ=255) gTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGcc > 1:1198887/1‑71 (MQ=255) ccttccGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGt > 1:3317849/1‑70 (MQ=255) cttccGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTg < 1:523627/70‑1 (MQ=255) ttccGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAg > 1:1737930/1‑71 (MQ=255) tccGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGc > 1:1608171/1‑71 (MQ=255) gggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCttt < 1:184408/71‑1 (MQ=255) gggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCtt > 1:249508/1‑70 (MQ=255) ggAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTa < 1:2946945/71‑1 (MQ=255) gAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTa < 1:2850196/70‑1 (MQ=255) gAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATAGTGCCGTGAGCTTTAg > 1:2989790/1‑71 (MQ=255) tCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGt < 1:886607/57‑1 (MQ=255) tCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTACCGCAATGGTGCCGTGAGCTTTAGTGCGa > 1:3688720/1‑71 (MQ=255) gATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGt < 1:1744033/71‑1 (MQ=255) gATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGt > 1:717948/1‑71 (MQ=255) gATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGt < 1:3649242/71‑1 (MQ=255) tACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCg < 1:4172644/71‑1 (MQ=255) ggCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGa > 1:3351819/1‑69 (MQ=255) gCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATc > 1:4065067/1‑70 (MQ=255) cGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCaa < 1:1483999/71‑1 (MQ=255) ttttttGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTc < 1:870381/63‑1 (MQ=255) ttttttGAAGTGATTCACTACTTCAGCAATGGTGCCGTGAGCtt < 1:837740/44‑1 (MQ=255) tttttGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAAt > 1:2158071/1‑69 (MQ=255) ttttGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAg > 1:1118981/1‑71 (MQ=255) ttttGAAGTGATTCACTACTTCCGCAATAGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAg > 1:4229211/1‑71 (MQ=255) tttGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAg < 1:3862379/44‑1 (MQ=255) aaGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGc < 1:1908811/70‑1 (MQ=255) gTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCgg < 1:1034027/71‑1 (MQ=255) gTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCgg > 1:1906842/1‑71 (MQ=255) tGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGAt < 1:1294050/56‑1 (MQ=255) tGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCgg < 1:4035691/70‑1 (MQ=255) gATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGa > 1:3155147/1‑41 (MQ=255) gATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCgg > 1:2253569/1‑69 (MQ=255) tCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc > 1:2369790/1‑71 (MQ=255) cACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAAc > 1:1029370/1‑70 (MQ=255) actactTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATaa > 1:3914047/1‑56 (MQ=255) ctTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGttt > 1:760287/1‑38 (MQ=255) ctTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGttt > 1:1332935/1‑38 (MQ=255) ctTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGttt > 1:651856/1‑38 (MQ=255) ctTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTAc < 1:3858859/71‑1 (MQ=255) ttCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATa < 1:75559/50‑1 (MQ=255) ccGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACaa < 1:100428/70‑1 (MQ=255) cGCAATAGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACt < 1:1803263/71‑1 (MQ=255) cGCAATAGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACt < 1:1808176/71‑1 (MQ=255) cAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAAt < 1:3234698/44‑1 (MQ=255) tGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGtt < 1:3370026/53‑1 (MQ=255) tGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGtt < 1:1423288/53‑1 (MQ=255) tGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCa < 1:2783724/58‑1 (MQ=255) | TGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTGCCGGTTAACCAGTACAACT > W3110S.gb/3161687‑3161821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |