| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,294,438 | T→C | 11.9% | L395S (TTG→TCG) | yraM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,294,438 | 0 | T | C | 11.9% | 146.5 / 12.5 | 59 | L395S (TTG→TCG) | yraM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (28/24); new base C (5/2); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
AAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGT > W3110S.gb/3294370‑3294504 | aaGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGc > 1:3746401/1‑71 (MQ=255) aGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGtt < 1:3640576/68‑1 (MQ=255) aGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGtt < 1:196578/68‑1 (MQ=255) cAGGTTCAGCAGGATGGCGCGAGTTTTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa < 1:2993395/71‑1 (MQ=255) cAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGGTGaa < 1:5264/71‑1 (MQ=255) cAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa < 1:96759/71‑1 (MQ=255) cAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGaa < 1:2049727/71‑1 (MQ=255) aGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAag < 1:1428363/71‑1 (MQ=255) ggTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa > 1:1667632/1‑69 (MQ=255) gTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAagag > 1:2435886/1‑71 (MQ=255) ttCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGc < 1:1824555/71‑1 (MQ=255) ttCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc < 1:1882524/71‑1 (MQ=255) cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGc > 1:3239278/1‑58 (MQ=255) agGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACt > 1:1346874/1‑71 (MQ=255) agGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACt > 1:1826305/1‑71 (MQ=255) ggATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTc < 1:3500066/71‑1 (MQ=255) tGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGc > 1:527204/1‑71 (MQ=255) tGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGaa > 1:2972201/1‑57 (MQ=255) tGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:4231753/1‑63 (MQ=255) tGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGGTGAAGAGTTGCTGAAGAGCAACACTCCGc > 1:3763275/1‑71 (MQ=255) gAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACg < 1:3883283/71‑1 (MQ=255) gTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCaa > 1:165409/1‑55 (MQ=255) gTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTa > 1:207743/1‑71 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa > 1:2127455/1‑46 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa > 1:11537/1‑46 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGc < 1:1543844/60‑1 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTg > 1:2650557/1‑71 (MQ=255) tgtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTg < 1:2856101/70‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTg < 1:4190508/39‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa > 1:2005857/1‑44 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAag > 1:1055475/1‑45 (MQ=255) ggtcggtcCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCAc > 1:1049212/1‑71 (MQ=255) tcggtcCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTg < 1:2732871/71‑1 (MQ=255) tcCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAAc > 1:2111931/1‑70 (MQ=255) ccGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAaca > 1:936759/1‑43 (MQ=255) cGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAg < 1:1356811/71‑1 (MQ=255) cGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAg > 1:582793/1‑71 (MQ=255) ttGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGcc > 1:849564/1‑71 (MQ=255) ttGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGACACTGAACCAGc > 1:2468068/1‑70 (MQ=255) gCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACt > 1:410627/1‑60 (MQ=255) gCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCg > 1:183856/1‑70 (MQ=255) gCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCg > 1:2608355/1‑70 (MQ=255) gAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGc < 1:1236069/54‑1 (MQ=255) aaaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgag < 1:2780933/69‑1 (MQ=255) aaaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgag < 1:549712/69‑1 (MQ=255) aaaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgag < 1:1781379/69‑1 (MQ=255) aaaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGaa > 1:3514662/1‑71 (MQ=255) aaaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGaa > 1:372755/1‑71 (MQ=255) aaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAat < 1:4259024/71‑1 (MQ=255) aaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAata < 1:2936480/71‑1 (MQ=255) aaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAata > 1:3486914/1‑71 (MQ=255) aTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGaa > 1:3534939/1‑55 (MQ=255) tAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCg < 1:638072/71‑1 (MQ=255) aCGTTGAAGAGTTGCTTAAGAGCAACACTCCGCTGAACGTACTGGc < 1:4023714/46‑1 (MQ=255) aCGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGc < 1:4026084/46‑1 (MQ=255) aCGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGc < 1:1029210/46‑1 (MQ=255) gTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGaaaa < 1:3573895/71‑1 (MQ=255) gAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGaaa < 1:108393/67‑1 (MQ=255) gAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATcg > 1:4142767/1‑71 (MQ=255) gagTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGt > 1:540566/1‑71 (MQ=255) agTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACt > 1:239702/1‑40 (MQ=255) | AAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAATCGCGT > W3110S.gb/3294370‑3294504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |